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Precise CCM1 gene correction and inactivation in patient‐derived endothelial cells: Modeling Knudson's two‐hit hypothesis in vitro

BACKGROUND: The CRISPR/Cas9 system has opened new perspectives to study the molecular basis of cerebral cavernous malformations (CCMs) in personalized disease models. However, precise genome editing in endothelial and other hard‐to‐transfect cells remains challenging. METHODS: In a proof‐of‐principl...

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Detalles Bibliográficos
Autores principales:	Spiegler, Stefanie, Rath, Matthias, Much, Christiane D., Sendtner, Barbara S., Felbor, Ute
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625102/ https://www.ncbi.nlm.nih.gov/pubmed/31124307 http://dx.doi.org/10.1002/mgg3.755

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