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Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells

Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor‐specific CD8(+) T cells. Carbon nanotubes (CNTs) have attract...

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Autores principales: Dong, Zhipeng, Wang, Qiyan, Huo, Ming, Zhang, Nanxia, Li, Bingxia, Li, Hongmei, Xu, Yisong, Chen, Meng, Hong, Hao, Wang, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625155/
https://www.ncbi.nlm.nih.gov/pubmed/31338275
http://dx.doi.org/10.1002/open.201900126
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author Dong, Zhipeng
Wang, Qiyan
Huo, Ming
Zhang, Nanxia
Li, Bingxia
Li, Hongmei
Xu, Yisong
Chen, Meng
Hong, Hao
Wang, Yue
author_facet Dong, Zhipeng
Wang, Qiyan
Huo, Ming
Zhang, Nanxia
Li, Bingxia
Li, Hongmei
Xu, Yisong
Chen, Meng
Hong, Hao
Wang, Yue
author_sort Dong, Zhipeng
collection PubMed
description Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor‐specific CD8(+) T cells. Carbon nanotubes (CNTs) have attracted great attention in biomedicine because of their unique physicochemical properties. In order to effectively deliver tumor antigens to DCs and trigger a strong anti‐tumor immune response, herein, a specific DCs target delivery system was assembled by using multi‐walled carbon nanotubes modified with mannose which can specifically bind to the mannose receptor on DCs membrane. Ovalbumin (OVA) as a model antigen, could be adsorbed on the surface of mannose modified multi‐walled carbon nanotubes (Man‐MWCNTs) with a large drug loading content. This nanotube‐antigen complex showed low cytotoxicity to DCs and was efficiently engulfed by DCs to induce DCs maturation and cytokine release in vitro, indicating that it could be a potent antigen‐adjuvant nanovector of efficient antigen delivery for therapeutic purpose.
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spelling pubmed-66251552019-07-23 Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells Dong, Zhipeng Wang, Qiyan Huo, Ming Zhang, Nanxia Li, Bingxia Li, Hongmei Xu, Yisong Chen, Meng Hong, Hao Wang, Yue ChemistryOpen Full Papers Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor‐specific CD8(+) T cells. Carbon nanotubes (CNTs) have attracted great attention in biomedicine because of their unique physicochemical properties. In order to effectively deliver tumor antigens to DCs and trigger a strong anti‐tumor immune response, herein, a specific DCs target delivery system was assembled by using multi‐walled carbon nanotubes modified with mannose which can specifically bind to the mannose receptor on DCs membrane. Ovalbumin (OVA) as a model antigen, could be adsorbed on the surface of mannose modified multi‐walled carbon nanotubes (Man‐MWCNTs) with a large drug loading content. This nanotube‐antigen complex showed low cytotoxicity to DCs and was efficiently engulfed by DCs to induce DCs maturation and cytokine release in vitro, indicating that it could be a potent antigen‐adjuvant nanovector of efficient antigen delivery for therapeutic purpose. John Wiley and Sons Inc. 2019-07-12 /pmc/articles/PMC6625155/ /pubmed/31338275 http://dx.doi.org/10.1002/open.201900126 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Dong, Zhipeng
Wang, Qiyan
Huo, Ming
Zhang, Nanxia
Li, Bingxia
Li, Hongmei
Xu, Yisong
Chen, Meng
Hong, Hao
Wang, Yue
Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title_full Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title_fullStr Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title_full_unstemmed Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title_short Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells
title_sort mannose‐modified multi‐walled carbon nanotubes as a delivery nanovector optimizing the antigen presentation of dendritic cells
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625155/
https://www.ncbi.nlm.nih.gov/pubmed/31338275
http://dx.doi.org/10.1002/open.201900126
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