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Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells
The dental follicle develops into the periodontal ligament, cementum and alveolar bone. Human dental follicle cells (hDFCs) are the precursor cells of periodontal development. Long non-coding RNAs (lncRNAs) have been revealed to be crucial factors that regulate a variety of biological processes; how...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625187/ https://www.ncbi.nlm.nih.gov/pubmed/31173189 http://dx.doi.org/10.3892/mmr.2019.10308 |
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author | Wu, Liping Deng, Lidi Hong, Hong Peng, Caixia Zhang, Xueqin Chen, Zhengyuan Ling, Junqi |
author_facet | Wu, Liping Deng, Lidi Hong, Hong Peng, Caixia Zhang, Xueqin Chen, Zhengyuan Ling, Junqi |
author_sort | Wu, Liping |
collection | PubMed |
description | The dental follicle develops into the periodontal ligament, cementum and alveolar bone. Human dental follicle cells (hDFCs) are the precursor cells of periodontal development. Long non-coding RNAs (lncRNAs) have been revealed to be crucial factors that regulate a variety of biological processes; however, whether lncRNAs serve a role in human periodontal development remains unknown. Therefore, the present study used microarrays to detect the differentially expressed lncRNAs and mRNAs between hDFCs and human periodontal ligament cells (hPDLCs). A total of 845 lncRNAs and 1,012 mRNAs were identified to be differentially expressed in hDFCs and hPDLCs (fold change >2.0 or <-2.0; P<0.05). Microarray data were validated by reverse transcription-quantitative polymerase chain reaction. Bioinformatics analyses, including gene ontology, pathway analysis and coding-non-coding gene co-expression network analysis, were performed to determine the functions of the differentially expressed lncRNAs and mRNAs. Bioinformatics analysis identified that a number of pathways may be associated with periodontal development, including the p53 and calcium signaling pathways. This analysis also revealed a number of lncRNAs, including NR_033932, T152410, ENST00000512129, ENST00000540293, uc021sxs.1 and ENST00000609146, which may serve important roles in the biological process of hDFCs. In addition, the lncRNA termed maternally expressed 3 (MEG3) was identified to be differentially expressed in hDFCs by reverse transcription-quantitative polymerase chain reaction. The knockdown of MEG3 was associated with a reduction of pluripotency makers in hDFCs. In conclusion, for the first time, to the best of our knowledge, the current study determined the different expression profiles of lncRNAs and mRNAs between hDFCs and hPDLCs. The observations made may provide a solid foundation for further research into the molecular mechanisms of lncRNAs in human periodontal development. |
format | Online Article Text |
id | pubmed-6625187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66251872019-07-31 Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells Wu, Liping Deng, Lidi Hong, Hong Peng, Caixia Zhang, Xueqin Chen, Zhengyuan Ling, Junqi Mol Med Rep Articles The dental follicle develops into the periodontal ligament, cementum and alveolar bone. Human dental follicle cells (hDFCs) are the precursor cells of periodontal development. Long non-coding RNAs (lncRNAs) have been revealed to be crucial factors that regulate a variety of biological processes; however, whether lncRNAs serve a role in human periodontal development remains unknown. Therefore, the present study used microarrays to detect the differentially expressed lncRNAs and mRNAs between hDFCs and human periodontal ligament cells (hPDLCs). A total of 845 lncRNAs and 1,012 mRNAs were identified to be differentially expressed in hDFCs and hPDLCs (fold change >2.0 or <-2.0; P<0.05). Microarray data were validated by reverse transcription-quantitative polymerase chain reaction. Bioinformatics analyses, including gene ontology, pathway analysis and coding-non-coding gene co-expression network analysis, were performed to determine the functions of the differentially expressed lncRNAs and mRNAs. Bioinformatics analysis identified that a number of pathways may be associated with periodontal development, including the p53 and calcium signaling pathways. This analysis also revealed a number of lncRNAs, including NR_033932, T152410, ENST00000512129, ENST00000540293, uc021sxs.1 and ENST00000609146, which may serve important roles in the biological process of hDFCs. In addition, the lncRNA termed maternally expressed 3 (MEG3) was identified to be differentially expressed in hDFCs by reverse transcription-quantitative polymerase chain reaction. The knockdown of MEG3 was associated with a reduction of pluripotency makers in hDFCs. In conclusion, for the first time, to the best of our knowledge, the current study determined the different expression profiles of lncRNAs and mRNAs between hDFCs and hPDLCs. The observations made may provide a solid foundation for further research into the molecular mechanisms of lncRNAs in human periodontal development. D.A. Spandidos 2019-08 2019-05-29 /pmc/articles/PMC6625187/ /pubmed/31173189 http://dx.doi.org/10.3892/mmr.2019.10308 Text en Copyright: © Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wu, Liping Deng, Lidi Hong, Hong Peng, Caixia Zhang, Xueqin Chen, Zhengyuan Ling, Junqi Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title | Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title_full | Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title_fullStr | Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title_full_unstemmed | Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title_short | Comparison of long non-coding RNA expression profiles in human dental follicle cells and human periodontal ligament cells |
title_sort | comparison of long non-coding rna expression profiles in human dental follicle cells and human periodontal ligament cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625187/ https://www.ncbi.nlm.nih.gov/pubmed/31173189 http://dx.doi.org/10.3892/mmr.2019.10308 |
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