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Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling

Puerarin is the major bioactive ingredient isolated from the dry root of Pueraria lobata, a plant used in traditional Chinese medicine. Puerarin has been used to treat diabetes and cataracts in China; however, its underlying mechanism of action remains unclear. The aim of the present study was to in...

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Autores principales: Zhang, Duzhen, Li, Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625395/
https://www.ncbi.nlm.nih.gov/pubmed/31173182
http://dx.doi.org/10.3892/mmr.2019.10320
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author Zhang, Duzhen
Li, Man
author_facet Zhang, Duzhen
Li, Man
author_sort Zhang, Duzhen
collection PubMed
description Puerarin is the major bioactive ingredient isolated from the dry root of Pueraria lobata, a plant used in traditional Chinese medicine. Puerarin has been used to treat diabetes and cataracts in China; however, its underlying mechanism of action remains unclear. The aim of the present study was to investigate the effectiveness and mechanism of puerarin in preventing cataracts in diabetic rats. Diabetes was induced by streptozocin (STZ) administration and rats were intraperitoneally injected with puerarin (25, 50 and 100 mg/kg). Blood glucose levels and cataract development were examined in the different experimental groups. In addition, the expression levels of markers associated with oxidative stress, including nuclear factor erythroid 2 like 2 (Nrf2) and heme oxygenase-1 (HO-1), were analyzed. The present results suggested that treatment with puerarin at 25, 50 and 100 mg/kg significantly reduced blood glucose levels and the incidence of cataract in STZ-induced diabetic rats. Additionally, puerarin treatment reduced oxidative stress, restoring the levels of malondialdehyde and glutathione, and the activity of glutathione peroxidase. Furthermore, puerarin administration decreased the expression levels of retinal vascular endothelial growth factor and interleukin-1β and increased the mRNA expression levels of Nrf2 and HO-1, thus inhibiting oxidative stress. The present findings suggested that puerarin had hypoglycemic effects and that it prevented cataract development and progression in diabetic rats by reducing oxidative stress through the Nrf2/HO-1 signaling pathway.
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spelling pubmed-66253952019-07-31 Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling Zhang, Duzhen Li, Man Mol Med Rep Articles Puerarin is the major bioactive ingredient isolated from the dry root of Pueraria lobata, a plant used in traditional Chinese medicine. Puerarin has been used to treat diabetes and cataracts in China; however, its underlying mechanism of action remains unclear. The aim of the present study was to investigate the effectiveness and mechanism of puerarin in preventing cataracts in diabetic rats. Diabetes was induced by streptozocin (STZ) administration and rats were intraperitoneally injected with puerarin (25, 50 and 100 mg/kg). Blood glucose levels and cataract development were examined in the different experimental groups. In addition, the expression levels of markers associated with oxidative stress, including nuclear factor erythroid 2 like 2 (Nrf2) and heme oxygenase-1 (HO-1), were analyzed. The present results suggested that treatment with puerarin at 25, 50 and 100 mg/kg significantly reduced blood glucose levels and the incidence of cataract in STZ-induced diabetic rats. Additionally, puerarin treatment reduced oxidative stress, restoring the levels of malondialdehyde and glutathione, and the activity of glutathione peroxidase. Furthermore, puerarin administration decreased the expression levels of retinal vascular endothelial growth factor and interleukin-1β and increased the mRNA expression levels of Nrf2 and HO-1, thus inhibiting oxidative stress. The present findings suggested that puerarin had hypoglycemic effects and that it prevented cataract development and progression in diabetic rats by reducing oxidative stress through the Nrf2/HO-1 signaling pathway. D.A. Spandidos 2019-08 2019-06-03 /pmc/articles/PMC6625395/ /pubmed/31173182 http://dx.doi.org/10.3892/mmr.2019.10320 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Duzhen
Li, Man
Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title_full Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title_fullStr Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title_full_unstemmed Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title_short Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO-1 signaling
title_sort puerarin prevents cataract development and progression in diabetic rats through nrf2/ho-1 signaling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625395/
https://www.ncbi.nlm.nih.gov/pubmed/31173182
http://dx.doi.org/10.3892/mmr.2019.10320
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