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Inhibitory effects of miR-26b-5p on thyroid cancer
In order to examine the inhibitory effects of microRNA (miR)-26b-5p on thyroid cancer (TC), the clinicopathological features and pathological tissues of 67 patients were collected. The expression levels of miR-26b-5p were detected in TC and paracarcinoma tissues by quantitative polymerase chain reac...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625412/ https://www.ncbi.nlm.nih.gov/pubmed/31173209 http://dx.doi.org/10.3892/mmr.2019.10315 |
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author | Zhou, Aiya Chen, Gengyu Cheng, Xiankui Zhang, Chi Xu, Hao Qi, Ming Chen, Xiao Wang, Tiantian Li, Leping |
author_facet | Zhou, Aiya Chen, Gengyu Cheng, Xiankui Zhang, Chi Xu, Hao Qi, Ming Chen, Xiao Wang, Tiantian Li, Leping |
author_sort | Zhou, Aiya |
collection | PubMed |
description | In order to examine the inhibitory effects of microRNA (miR)-26b-5p on thyroid cancer (TC), the clinicopathological features and pathological tissues of 67 patients were collected. The expression levels of miR-26b-5p were detected in TC and paracarcinoma tissues by quantitative polymerase chain reaction, and the association between miR-26b-5p expression and the clinicopathological features of the patients was analyzed using t-test or one-way analysis of variance. In addition, B-CPAP TC cells were infected with a lentivirus to induce miR-26b-5p overexpression and proliferation was detected by Cell Counting kit-8. Subsequently, migration and invasion were detected by Transwell and Matrigel assays, respectively, and the molecular mechanism of action was investigated by western blotting. The results demonstrated that the expression levels of miR-26b-5p were significantly lower in TC tissues compared with paracarcinoma tissues (P<0.01), and miR-26b-5p was associated with lymph node metastasis (P<0.05). In addition, overexpression of miR-26b-5p inhibited the proliferation, invasion and migration of B-CPAP cells. Western blot analysis demonstrated that the protein expression levels of phosphorylated glycogen synthase kinase-3β (pGsk-3β) were decreased, and the expression of β-catenin was decreased in B-CPAP cells overexpressing miR-26b-5p. These results demonstrated that miR-26b-5p may exert antitumor activity. In addition, at the molecular level, these effects may be associated with the Gsk-3β/β-catenin pathway. |
format | Online Article Text |
id | pubmed-6625412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66254122019-07-31 Inhibitory effects of miR-26b-5p on thyroid cancer Zhou, Aiya Chen, Gengyu Cheng, Xiankui Zhang, Chi Xu, Hao Qi, Ming Chen, Xiao Wang, Tiantian Li, Leping Mol Med Rep Articles In order to examine the inhibitory effects of microRNA (miR)-26b-5p on thyroid cancer (TC), the clinicopathological features and pathological tissues of 67 patients were collected. The expression levels of miR-26b-5p were detected in TC and paracarcinoma tissues by quantitative polymerase chain reaction, and the association between miR-26b-5p expression and the clinicopathological features of the patients was analyzed using t-test or one-way analysis of variance. In addition, B-CPAP TC cells were infected with a lentivirus to induce miR-26b-5p overexpression and proliferation was detected by Cell Counting kit-8. Subsequently, migration and invasion were detected by Transwell and Matrigel assays, respectively, and the molecular mechanism of action was investigated by western blotting. The results demonstrated that the expression levels of miR-26b-5p were significantly lower in TC tissues compared with paracarcinoma tissues (P<0.01), and miR-26b-5p was associated with lymph node metastasis (P<0.05). In addition, overexpression of miR-26b-5p inhibited the proliferation, invasion and migration of B-CPAP cells. Western blot analysis demonstrated that the protein expression levels of phosphorylated glycogen synthase kinase-3β (pGsk-3β) were decreased, and the expression of β-catenin was decreased in B-CPAP cells overexpressing miR-26b-5p. These results demonstrated that miR-26b-5p may exert antitumor activity. In addition, at the molecular level, these effects may be associated with the Gsk-3β/β-catenin pathway. D.A. Spandidos 2019-08 2019-05-31 /pmc/articles/PMC6625412/ /pubmed/31173209 http://dx.doi.org/10.3892/mmr.2019.10315 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhou, Aiya Chen, Gengyu Cheng, Xiankui Zhang, Chi Xu, Hao Qi, Ming Chen, Xiao Wang, Tiantian Li, Leping Inhibitory effects of miR-26b-5p on thyroid cancer |
title | Inhibitory effects of miR-26b-5p on thyroid cancer |
title_full | Inhibitory effects of miR-26b-5p on thyroid cancer |
title_fullStr | Inhibitory effects of miR-26b-5p on thyroid cancer |
title_full_unstemmed | Inhibitory effects of miR-26b-5p on thyroid cancer |
title_short | Inhibitory effects of miR-26b-5p on thyroid cancer |
title_sort | inhibitory effects of mir-26b-5p on thyroid cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625412/ https://www.ncbi.nlm.nih.gov/pubmed/31173209 http://dx.doi.org/10.3892/mmr.2019.10315 |
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