Cargando…
Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling
A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Me...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625442/ https://www.ncbi.nlm.nih.gov/pubmed/31257470 http://dx.doi.org/10.3892/mmr.2019.10379 |
_version_ | 1783434417071456256 |
---|---|
author | Li, Jianhui Jia, Junjun He, Ning Jiang, Li Yu, Hao Li, Haoyu Peng, Yifan Xie, Haiyang Zhou, Lin Zheng, Shusen |
author_facet | Li, Jianhui Jia, Junjun He, Ning Jiang, Li Yu, Hao Li, Haoyu Peng, Yifan Xie, Haiyang Zhou, Lin Zheng, Shusen |
author_sort | Li, Jianhui |
collection | PubMed |
description | A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 12 rats were randomly assigned into two groups (n=6 for each group): The kidney-liver perfusion (KL) group and liver perfusion (LP) group. Perfusate samples were collected during the perfusion process for the analysis of pH, K+ and liver function. Liver tissues were obtained for the evaluation of adenosine triphosphate (ATP), terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling and immunohistochemistry of Ki67. Cell cycle inhibitors, apoptosis-associated genes and signal transducer and activator of transcription 3 (Stat3) were analyzed using quantitative polymerase chain reaction and western blot analysis. Results: Overall pH and K(+) values of the KL group were significantly different from the LP group and more stable; aspartate aminotransferase, alanine transaminase and lactate dehydrogenase levels increased progressively over time in the LP group and were significantly different at different time points compared with pre-perfusion levels and the KL group, which suggested the KL group was superior to the LP group. In addition, KL reduced portal vein resistance and was associated with lower ATP consumption compared with the LP group. Furthermore, liver proliferation was upregulated with the upregulation of the interleukin 6 (IL-6)/Stat3 signaling pathway in KL compared with LP. The present study revealed for the first time that KL and hypothermic machine perfusion demonstrated a more proactive repair capability by maintaining liver regeneration via the upregulation of the IL-6/Stat3 signaling pathway. |
format | Online Article Text |
id | pubmed-6625442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66254422019-07-31 Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling Li, Jianhui Jia, Junjun He, Ning Jiang, Li Yu, Hao Li, Haoyu Peng, Yifan Xie, Haiyang Zhou, Lin Zheng, Shusen Mol Med Rep Articles A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 12 rats were randomly assigned into two groups (n=6 for each group): The kidney-liver perfusion (KL) group and liver perfusion (LP) group. Perfusate samples were collected during the perfusion process for the analysis of pH, K+ and liver function. Liver tissues were obtained for the evaluation of adenosine triphosphate (ATP), terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling and immunohistochemistry of Ki67. Cell cycle inhibitors, apoptosis-associated genes and signal transducer and activator of transcription 3 (Stat3) were analyzed using quantitative polymerase chain reaction and western blot analysis. Results: Overall pH and K(+) values of the KL group were significantly different from the LP group and more stable; aspartate aminotransferase, alanine transaminase and lactate dehydrogenase levels increased progressively over time in the LP group and were significantly different at different time points compared with pre-perfusion levels and the KL group, which suggested the KL group was superior to the LP group. In addition, KL reduced portal vein resistance and was associated with lower ATP consumption compared with the LP group. Furthermore, liver proliferation was upregulated with the upregulation of the interleukin 6 (IL-6)/Stat3 signaling pathway in KL compared with LP. The present study revealed for the first time that KL and hypothermic machine perfusion demonstrated a more proactive repair capability by maintaining liver regeneration via the upregulation of the IL-6/Stat3 signaling pathway. D.A. Spandidos 2019-08 2019-06-12 /pmc/articles/PMC6625442/ /pubmed/31257470 http://dx.doi.org/10.3892/mmr.2019.10379 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Jianhui Jia, Junjun He, Ning Jiang, Li Yu, Hao Li, Haoyu Peng, Yifan Xie, Haiyang Zhou, Lin Zheng, Shusen Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title | Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title_full | Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title_fullStr | Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title_full_unstemmed | Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title_short | Combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL-6/Stat3 signaling |
title_sort | combined kidney-liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of il-6/stat3 signaling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625442/ https://www.ncbi.nlm.nih.gov/pubmed/31257470 http://dx.doi.org/10.3892/mmr.2019.10379 |
work_keys_str_mv | AT lijianhui combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT jiajunjun combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT hening combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT jiangli combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT yuhao combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT lihaoyu combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT pengyifan combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT xiehaiyang combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT zhoulin combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling AT zhengshusen combinedkidneyliverperfusionenhancestheproliferationeffectsofhypothermicperfusiononlivergraftsviaupregulationofil6stat3signaling |