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Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)

BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATE...

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Autores principales: Yi, Jing, Zhou, Ling-yu, Yi, Yun-yun, Zhu, Xin, Su, Xiao-yu, Zhao, Qian, Lin, Jiang, Qian, Jun, Deng, Zhao-qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625577/
https://www.ncbi.nlm.nih.gov/pubmed/31271156
http://dx.doi.org/10.12659/MSM.914352
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author Yi, Jing
Zhou, Ling-yu
Yi, Yun-yun
Zhu, Xin
Su, Xiao-yu
Zhao, Qian
Lin, Jiang
Qian, Jun
Deng, Zhao-qun
author_facet Yi, Jing
Zhou, Ling-yu
Yi, Yun-yun
Zhu, Xin
Su, Xiao-yu
Zhao, Qian
Lin, Jiang
Qian, Jun
Deng, Zhao-qun
author_sort Yi, Jing
collection PubMed
description BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATERIAL/METHODS: The expression of the POU5F1B transcript was evaluated in 175 newly diagnosed AML patients and 39 healthy controls by use of real-time quantitative PCR (RQ-PCR). RESULTS: POU5F1B was underexpressed in AML compared with controls (P<0.001). The receiver operating characteristic (ROC) curve revealed that the POU5F1B transcript level was able to differentiate AML patients from healthy individuals (AUC=0.682). In non-APL AML patients, the POU5F1B(low) group had significantly higher WBC than the POU5F1B(high) group (20.2×10(9) vs. 4.6×10(9) L(−1), P=0.021). Among whole-cohort AML, non-APL AML, and intermediate-risk AML, POU5F1B(high) patients had obviously higher complete remission (CR) rates than POU5F1B(low) patients (P=0.012, P=0.012 and P=0.027). In addition, Kaplan-Meier analysis demonstrated better overall survival (OS, P=0.019, P=0.007 and P=0.046, respectively) in POU5F1B(high) patients compared with POU5F1B(low) patients. Furthermore, in multivariate survival analysis, POU5F1B was independently associated with OS in non-APL AML patients and intermediate-risk AML as a favorable prognostic factor. CONCLUSIONS: POU5F1B was frequently underexpressed in AML, and might contribute to the diagnosis and prognosis of AML.
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spelling pubmed-66255772019-07-30 Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) Yi, Jing Zhou, Ling-yu Yi, Yun-yun Zhu, Xin Su, Xiao-yu Zhao, Qian Lin, Jiang Qian, Jun Deng, Zhao-qun Med Sci Monit Lab/In Vitro Research BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATERIAL/METHODS: The expression of the POU5F1B transcript was evaluated in 175 newly diagnosed AML patients and 39 healthy controls by use of real-time quantitative PCR (RQ-PCR). RESULTS: POU5F1B was underexpressed in AML compared with controls (P<0.001). The receiver operating characteristic (ROC) curve revealed that the POU5F1B transcript level was able to differentiate AML patients from healthy individuals (AUC=0.682). In non-APL AML patients, the POU5F1B(low) group had significantly higher WBC than the POU5F1B(high) group (20.2×10(9) vs. 4.6×10(9) L(−1), P=0.021). Among whole-cohort AML, non-APL AML, and intermediate-risk AML, POU5F1B(high) patients had obviously higher complete remission (CR) rates than POU5F1B(low) patients (P=0.012, P=0.012 and P=0.027). In addition, Kaplan-Meier analysis demonstrated better overall survival (OS, P=0.019, P=0.007 and P=0.046, respectively) in POU5F1B(high) patients compared with POU5F1B(low) patients. Furthermore, in multivariate survival analysis, POU5F1B was independently associated with OS in non-APL AML patients and intermediate-risk AML as a favorable prognostic factor. CONCLUSIONS: POU5F1B was frequently underexpressed in AML, and might contribute to the diagnosis and prognosis of AML. International Scientific Literature, Inc. 2019-07-04 /pmc/articles/PMC6625577/ /pubmed/31271156 http://dx.doi.org/10.12659/MSM.914352 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Yi, Jing
Zhou, Ling-yu
Yi, Yun-yun
Zhu, Xin
Su, Xiao-yu
Zhao, Qian
Lin, Jiang
Qian, Jun
Deng, Zhao-qun
Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title_full Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title_fullStr Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title_full_unstemmed Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title_short Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
title_sort low expression of pseudogene pou5f1b affects diagnosis and prognosis in acute myeloid leukemia (aml)
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625577/
https://www.ncbi.nlm.nih.gov/pubmed/31271156
http://dx.doi.org/10.12659/MSM.914352
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