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Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML)
BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATE...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625577/ https://www.ncbi.nlm.nih.gov/pubmed/31271156 http://dx.doi.org/10.12659/MSM.914352 |
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author | Yi, Jing Zhou, Ling-yu Yi, Yun-yun Zhu, Xin Su, Xiao-yu Zhao, Qian Lin, Jiang Qian, Jun Deng, Zhao-qun |
author_facet | Yi, Jing Zhou, Ling-yu Yi, Yun-yun Zhu, Xin Su, Xiao-yu Zhao, Qian Lin, Jiang Qian, Jun Deng, Zhao-qun |
author_sort | Yi, Jing |
collection | PubMed |
description | BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATERIAL/METHODS: The expression of the POU5F1B transcript was evaluated in 175 newly diagnosed AML patients and 39 healthy controls by use of real-time quantitative PCR (RQ-PCR). RESULTS: POU5F1B was underexpressed in AML compared with controls (P<0.001). The receiver operating characteristic (ROC) curve revealed that the POU5F1B transcript level was able to differentiate AML patients from healthy individuals (AUC=0.682). In non-APL AML patients, the POU5F1B(low) group had significantly higher WBC than the POU5F1B(high) group (20.2×10(9) vs. 4.6×10(9) L(−1), P=0.021). Among whole-cohort AML, non-APL AML, and intermediate-risk AML, POU5F1B(high) patients had obviously higher complete remission (CR) rates than POU5F1B(low) patients (P=0.012, P=0.012 and P=0.027). In addition, Kaplan-Meier analysis demonstrated better overall survival (OS, P=0.019, P=0.007 and P=0.046, respectively) in POU5F1B(high) patients compared with POU5F1B(low) patients. Furthermore, in multivariate survival analysis, POU5F1B was independently associated with OS in non-APL AML patients and intermediate-risk AML as a favorable prognostic factor. CONCLUSIONS: POU5F1B was frequently underexpressed in AML, and might contribute to the diagnosis and prognosis of AML. |
format | Online Article Text |
id | pubmed-6625577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66255772019-07-30 Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) Yi, Jing Zhou, Ling-yu Yi, Yun-yun Zhu, Xin Su, Xiao-yu Zhao, Qian Lin, Jiang Qian, Jun Deng, Zhao-qun Med Sci Monit Lab/In Vitro Research BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML. MATERIAL/METHODS: The expression of the POU5F1B transcript was evaluated in 175 newly diagnosed AML patients and 39 healthy controls by use of real-time quantitative PCR (RQ-PCR). RESULTS: POU5F1B was underexpressed in AML compared with controls (P<0.001). The receiver operating characteristic (ROC) curve revealed that the POU5F1B transcript level was able to differentiate AML patients from healthy individuals (AUC=0.682). In non-APL AML patients, the POU5F1B(low) group had significantly higher WBC than the POU5F1B(high) group (20.2×10(9) vs. 4.6×10(9) L(−1), P=0.021). Among whole-cohort AML, non-APL AML, and intermediate-risk AML, POU5F1B(high) patients had obviously higher complete remission (CR) rates than POU5F1B(low) patients (P=0.012, P=0.012 and P=0.027). In addition, Kaplan-Meier analysis demonstrated better overall survival (OS, P=0.019, P=0.007 and P=0.046, respectively) in POU5F1B(high) patients compared with POU5F1B(low) patients. Furthermore, in multivariate survival analysis, POU5F1B was independently associated with OS in non-APL AML patients and intermediate-risk AML as a favorable prognostic factor. CONCLUSIONS: POU5F1B was frequently underexpressed in AML, and might contribute to the diagnosis and prognosis of AML. International Scientific Literature, Inc. 2019-07-04 /pmc/articles/PMC6625577/ /pubmed/31271156 http://dx.doi.org/10.12659/MSM.914352 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Lab/In Vitro Research Yi, Jing Zhou, Ling-yu Yi, Yun-yun Zhu, Xin Su, Xiao-yu Zhao, Qian Lin, Jiang Qian, Jun Deng, Zhao-qun Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title | Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title_full | Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title_fullStr | Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title_full_unstemmed | Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title_short | Low Expression of Pseudogene POU5F1B Affects Diagnosis and Prognosis in Acute Myeloid Leukemia (AML) |
title_sort | low expression of pseudogene pou5f1b affects diagnosis and prognosis in acute myeloid leukemia (aml) |
topic | Lab/In Vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625577/ https://www.ncbi.nlm.nih.gov/pubmed/31271156 http://dx.doi.org/10.12659/MSM.914352 |
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