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A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis
The intracellular accommodation structures formed by plant cells to host arbuscular mycorrhiza fungi and biotrophic hyphal pathogens are cytologically similar. Therefore we investigated whether these interactions build on an overlapping genetic framework. In legumes, the malectin-like domain leucine...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625732/ https://www.ncbi.nlm.nih.gov/pubmed/31299058 http://dx.doi.org/10.1371/journal.ppat.1007747 |
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author | Ried, Martina Katharina Banhara, Aline Hwu, Fang-Yu Binder, Andreas Gust, Andrea A. Höfle, Caroline Hückelhoven, Ralph Nürnberger, Thorsten Parniske, Martin |
author_facet | Ried, Martina Katharina Banhara, Aline Hwu, Fang-Yu Binder, Andreas Gust, Andrea A. Höfle, Caroline Hückelhoven, Ralph Nürnberger, Thorsten Parniske, Martin |
author_sort | Ried, Martina Katharina |
collection | PubMed |
description | The intracellular accommodation structures formed by plant cells to host arbuscular mycorrhiza fungi and biotrophic hyphal pathogens are cytologically similar. Therefore we investigated whether these interactions build on an overlapping genetic framework. In legumes, the malectin-like domain leucine-rich repeat receptor kinase SYMRK, the cation channel POLLUX and members of the nuclear pore NUP107-160 subcomplex are essential for symbiotic signal transduction and arbuscular mycorrhiza development. We identified members of these three groups in Arabidopsis thaliana and explored their impact on the interaction with the oomycete downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa). We report that mutations in the corresponding genes reduced the reproductive success of Hpa as determined by sporangiophore and spore counts. We discovered that a developmental transition of haustorial shape occurred significantly earlier and at higher frequency in the mutants. Analysis of the multiplication of extracellular bacterial pathogens, Hpa-induced cell death or callose accumulation, as well as Hpa- or flg22-induced defence marker gene expression, did not reveal any traces of constitutive or exacerbated defence responses. These findings point towards an overlap between the plant genetic toolboxes involved in the interaction with biotrophic intracellular hyphal symbionts and pathogens in terms of the gene families involved. |
format | Online Article Text |
id | pubmed-6625732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66257322019-07-25 A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis Ried, Martina Katharina Banhara, Aline Hwu, Fang-Yu Binder, Andreas Gust, Andrea A. Höfle, Caroline Hückelhoven, Ralph Nürnberger, Thorsten Parniske, Martin PLoS Pathog Research Article The intracellular accommodation structures formed by plant cells to host arbuscular mycorrhiza fungi and biotrophic hyphal pathogens are cytologically similar. Therefore we investigated whether these interactions build on an overlapping genetic framework. In legumes, the malectin-like domain leucine-rich repeat receptor kinase SYMRK, the cation channel POLLUX and members of the nuclear pore NUP107-160 subcomplex are essential for symbiotic signal transduction and arbuscular mycorrhiza development. We identified members of these three groups in Arabidopsis thaliana and explored their impact on the interaction with the oomycete downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa). We report that mutations in the corresponding genes reduced the reproductive success of Hpa as determined by sporangiophore and spore counts. We discovered that a developmental transition of haustorial shape occurred significantly earlier and at higher frequency in the mutants. Analysis of the multiplication of extracellular bacterial pathogens, Hpa-induced cell death or callose accumulation, as well as Hpa- or flg22-induced defence marker gene expression, did not reveal any traces of constitutive or exacerbated defence responses. These findings point towards an overlap between the plant genetic toolboxes involved in the interaction with biotrophic intracellular hyphal symbionts and pathogens in terms of the gene families involved. Public Library of Science 2019-07-12 /pmc/articles/PMC6625732/ /pubmed/31299058 http://dx.doi.org/10.1371/journal.ppat.1007747 Text en © 2019 Ried et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ried, Martina Katharina Banhara, Aline Hwu, Fang-Yu Binder, Andreas Gust, Andrea A. Höfle, Caroline Hückelhoven, Ralph Nürnberger, Thorsten Parniske, Martin A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title | A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title_full | A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title_fullStr | A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title_full_unstemmed | A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title_short | A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis |
title_sort | set of arabidopsis genes involved in the accommodation of the downy mildew pathogen hyaloperonospora arabidopsidis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625732/ https://www.ncbi.nlm.nih.gov/pubmed/31299058 http://dx.doi.org/10.1371/journal.ppat.1007747 |
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