Cargando…
First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study
The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein–protein interactions has increased during the last years. As the technique is now becoming routine for...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625963/ https://www.ncbi.nlm.nih.gov/pubmed/31045356 http://dx.doi.org/10.1021/acs.analchem.9b00658 |
_version_ | 1783434473110503424 |
---|---|
author | Iacobucci, Claudio Piotrowski, Christine Aebersold, Ruedi Amaral, Bruno C. Andrews, Philip Bernfur, Katja Borchers, Christoph Brodie, Nicolas I. Bruce, James E. Cao, Yong Chaignepain, Stéphane Chavez, Juan D. Claverol, Stéphane Cox, Jürgen Davis, Trisha Degliesposti, Gianluca Dong, Meng-Qiu Edinger, Nufar Emanuelsson, Cecilia Gay, Marina Götze, Michael Gomes-Neto, Francisco Gozzo, Fabio C. Gutierrez, Craig Haupt, Caroline Heck, Albert J. R. Herzog, Franz Huang, Lan Hoopmann, Michael R. Kalisman, Nir Klykov, Oleg Kukačka, Zdeněk Liu, Fan MacCoss, Michael J. Mechtler, Karl Mesika, Ravit Moritz, Robert L. Nagaraj, Nagarjuna Nesati, Victor Neves-Ferreira, Ana G. C. Ninnis, Robert Novák, Petr O’Reilly, Francis J. Pelzing, Matthias Petrotchenko, Evgeniy Piersimoni, Lolita Plasencia, Manolo Pukala, Tara Rand, Kasper D. Rappsilber, Juri Reichmann, Dana Sailer, Carolin Sarnowski, Chris P. Scheltema, Richard A. Schmidt, Carla Schriemer, David C. Shi, Yi Skehel, J. Mark Slavin, Moriya Sobott, Frank Solis-Mezarino, Victor Stephanowitz, Heike Stengel, Florian Stieger, Christian E. Trabjerg, Esben Trnka, Michael Vilaseca, Marta Viner, Rosa Xiang, Yufei Yilmaz, Sule Zelter, Alex Ziemianowicz, Daniel Leitner, Alexander Sinz, Andrea |
author_facet | Iacobucci, Claudio Piotrowski, Christine Aebersold, Ruedi Amaral, Bruno C. Andrews, Philip Bernfur, Katja Borchers, Christoph Brodie, Nicolas I. Bruce, James E. Cao, Yong Chaignepain, Stéphane Chavez, Juan D. Claverol, Stéphane Cox, Jürgen Davis, Trisha Degliesposti, Gianluca Dong, Meng-Qiu Edinger, Nufar Emanuelsson, Cecilia Gay, Marina Götze, Michael Gomes-Neto, Francisco Gozzo, Fabio C. Gutierrez, Craig Haupt, Caroline Heck, Albert J. R. Herzog, Franz Huang, Lan Hoopmann, Michael R. Kalisman, Nir Klykov, Oleg Kukačka, Zdeněk Liu, Fan MacCoss, Michael J. Mechtler, Karl Mesika, Ravit Moritz, Robert L. Nagaraj, Nagarjuna Nesati, Victor Neves-Ferreira, Ana G. C. Ninnis, Robert Novák, Petr O’Reilly, Francis J. Pelzing, Matthias Petrotchenko, Evgeniy Piersimoni, Lolita Plasencia, Manolo Pukala, Tara Rand, Kasper D. Rappsilber, Juri Reichmann, Dana Sailer, Carolin Sarnowski, Chris P. Scheltema, Richard A. Schmidt, Carla Schriemer, David C. Shi, Yi Skehel, J. Mark Slavin, Moriya Sobott, Frank Solis-Mezarino, Victor Stephanowitz, Heike Stengel, Florian Stieger, Christian E. Trabjerg, Esben Trnka, Michael Vilaseca, Marta Viner, Rosa Xiang, Yufei Yilmaz, Sule Zelter, Alex Ziemianowicz, Daniel Leitner, Alexander Sinz, Andrea |
author_sort | Iacobucci, Claudio |
collection | PubMed |
description | The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein–protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results. |
format | Online Article Text |
id | pubmed-6625963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-66259632019-07-12 First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study Iacobucci, Claudio Piotrowski, Christine Aebersold, Ruedi Amaral, Bruno C. Andrews, Philip Bernfur, Katja Borchers, Christoph Brodie, Nicolas I. Bruce, James E. Cao, Yong Chaignepain, Stéphane Chavez, Juan D. Claverol, Stéphane Cox, Jürgen Davis, Trisha Degliesposti, Gianluca Dong, Meng-Qiu Edinger, Nufar Emanuelsson, Cecilia Gay, Marina Götze, Michael Gomes-Neto, Francisco Gozzo, Fabio C. Gutierrez, Craig Haupt, Caroline Heck, Albert J. R. Herzog, Franz Huang, Lan Hoopmann, Michael R. Kalisman, Nir Klykov, Oleg Kukačka, Zdeněk Liu, Fan MacCoss, Michael J. Mechtler, Karl Mesika, Ravit Moritz, Robert L. Nagaraj, Nagarjuna Nesati, Victor Neves-Ferreira, Ana G. C. Ninnis, Robert Novák, Petr O’Reilly, Francis J. Pelzing, Matthias Petrotchenko, Evgeniy Piersimoni, Lolita Plasencia, Manolo Pukala, Tara Rand, Kasper D. Rappsilber, Juri Reichmann, Dana Sailer, Carolin Sarnowski, Chris P. Scheltema, Richard A. Schmidt, Carla Schriemer, David C. Shi, Yi Skehel, J. Mark Slavin, Moriya Sobott, Frank Solis-Mezarino, Victor Stephanowitz, Heike Stengel, Florian Stieger, Christian E. Trabjerg, Esben Trnka, Michael Vilaseca, Marta Viner, Rosa Xiang, Yufei Yilmaz, Sule Zelter, Alex Ziemianowicz, Daniel Leitner, Alexander Sinz, Andrea Anal Chem Article The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein–protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results. 2019-06-04 2019-05-22 /pmc/articles/PMC6625963/ /pubmed/31045356 http://dx.doi.org/10.1021/acs.analchem.9b00658 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article published under an ACS AuthorChoice License (https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Iacobucci, Claudio Piotrowski, Christine Aebersold, Ruedi Amaral, Bruno C. Andrews, Philip Bernfur, Katja Borchers, Christoph Brodie, Nicolas I. Bruce, James E. Cao, Yong Chaignepain, Stéphane Chavez, Juan D. Claverol, Stéphane Cox, Jürgen Davis, Trisha Degliesposti, Gianluca Dong, Meng-Qiu Edinger, Nufar Emanuelsson, Cecilia Gay, Marina Götze, Michael Gomes-Neto, Francisco Gozzo, Fabio C. Gutierrez, Craig Haupt, Caroline Heck, Albert J. R. Herzog, Franz Huang, Lan Hoopmann, Michael R. Kalisman, Nir Klykov, Oleg Kukačka, Zdeněk Liu, Fan MacCoss, Michael J. Mechtler, Karl Mesika, Ravit Moritz, Robert L. Nagaraj, Nagarjuna Nesati, Victor Neves-Ferreira, Ana G. C. Ninnis, Robert Novák, Petr O’Reilly, Francis J. Pelzing, Matthias Petrotchenko, Evgeniy Piersimoni, Lolita Plasencia, Manolo Pukala, Tara Rand, Kasper D. Rappsilber, Juri Reichmann, Dana Sailer, Carolin Sarnowski, Chris P. Scheltema, Richard A. Schmidt, Carla Schriemer, David C. Shi, Yi Skehel, J. Mark Slavin, Moriya Sobott, Frank Solis-Mezarino, Victor Stephanowitz, Heike Stengel, Florian Stieger, Christian E. Trabjerg, Esben Trnka, Michael Vilaseca, Marta Viner, Rosa Xiang, Yufei Yilmaz, Sule Zelter, Alex Ziemianowicz, Daniel Leitner, Alexander Sinz, Andrea First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study |
title | First Community-Wide, Comparative Cross-Linking Mass Spectrometry
Study |
title_full | First Community-Wide, Comparative Cross-Linking Mass Spectrometry
Study |
title_fullStr | First Community-Wide, Comparative Cross-Linking Mass Spectrometry
Study |
title_full_unstemmed | First Community-Wide, Comparative Cross-Linking Mass Spectrometry
Study |
title_short | First Community-Wide, Comparative Cross-Linking Mass Spectrometry
Study |
title_sort | first community-wide, comparative cross-linking mass spectrometry
study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625963/ https://www.ncbi.nlm.nih.gov/pubmed/31045356 http://dx.doi.org/10.1021/acs.analchem.9b00658 |
work_keys_str_mv | AT iacobucciclaudio firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT piotrowskichristine firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT aebersoldruedi firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT amaralbrunoc firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT andrewsphilip firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT bernfurkatja firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT borcherschristoph firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT brodienicolasi firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT brucejamese firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT caoyong firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT chaignepainstephane firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT chavezjuand firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT claverolstephane firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT coxjurgen firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT davistrisha firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT degliespostigianluca firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT dongmengqiu firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT edingernufar firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT emanuelssoncecilia firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT gaymarina firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT gotzemichael firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT gomesnetofrancisco firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT gozzofabioc firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT gutierrezcraig firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT hauptcaroline firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT heckalbertjr firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT herzogfranz firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT huanglan firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT hoopmannmichaelr firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT kalismannir firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT klykovoleg firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT kukackazdenek firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT liufan firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT maccossmichaelj firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT mechtlerkarl firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT mesikaravit firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT moritzrobertl firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT nagarajnagarjuna firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT nesativictor firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT nevesferreiraanagc firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT ninnisrobert firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT novakpetr firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT oreillyfrancisj firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT pelzingmatthias firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT petrotchenkoevgeniy firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT piersimonilolita firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT plasenciamanolo firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT pukalatara firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT randkasperd firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT rappsilberjuri firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT reichmanndana firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT sailercarolin firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT sarnowskichrisp firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT scheltemaricharda firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT schmidtcarla firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT schriemerdavidc firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT shiyi firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT skeheljmark firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT slavinmoriya firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT sobottfrank firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT solismezarinovictor firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT stephanowitzheike firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT stengelflorian firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT stiegerchristiane firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT trabjergesben firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT trnkamichael firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT vilasecamarta firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT vinerrosa firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT xiangyufei firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT yilmazsule firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT zelteralex firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT ziemianowiczdaniel firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT leitneralexander firstcommunitywidecomparativecrosslinkingmassspectrometrystudy AT sinzandrea firstcommunitywidecomparativecrosslinkingmassspectrometrystudy |