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Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()

Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties...

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Autores principales: Chae, Yeon Ji, Kim, Jinil, Heo, Hwon, Woo, Chul-Woong, Kim, Sang-Tae, Kim, Min Jung, Choi, Jong Rip, Kim, Dae Hee, Woo, Dong-Cheol, Kim, Kyung Won, Choi, Yoonseok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626083/
https://www.ncbi.nlm.nih.gov/pubmed/31302474
http://dx.doi.org/10.1016/j.tranon.2019.06.006
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author Chae, Yeon Ji
Kim, Jinil
Heo, Hwon
Woo, Chul-Woong
Kim, Sang-Tae
Kim, Min Jung
Choi, Jong Rip
Kim, Dae Hee
Woo, Dong-Cheol
Kim, Kyung Won
Choi, Yoonseok
author_facet Chae, Yeon Ji
Kim, Jinil
Heo, Hwon
Woo, Chul-Woong
Kim, Sang-Tae
Kim, Min Jung
Choi, Jong Rip
Kim, Dae Hee
Woo, Dong-Cheol
Kim, Kyung Won
Choi, Yoonseok
author_sort Chae, Yeon Ji
collection PubMed
description Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties in conducting colorectal imaging. The purpose of this present study was to investigate the feasibility of using magnetic resonance colonography (MRC) to evaluate the efficacy of an ICI, an anti-PD-L1 antibody, in an orthotopic colorectal cancer mouse model. The mouse model was generated by the engraftment of colorectal cancer cells into the submucosal layer of the colon. Anti-cancer efficacy was assessed by tumor volume and metastatic tumor number analyses, and these values were significantly lower in the PD-L1 antibody-treated group compared to the controls. Histological analyses using H&E and Ki-67 immunohistochemical staining confirmed a highly efficacious tumor growth inhibition and enhanced infiltration by CD4+ and CD8+ lymphocytes in the PD-L1 antibody-treated group. We conclude that MRC has the potential to be used for ICI efficacy assessments against orthotopic colorectal cancer mouse model.
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spelling pubmed-66260832019-07-23 Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() Chae, Yeon Ji Kim, Jinil Heo, Hwon Woo, Chul-Woong Kim, Sang-Tae Kim, Min Jung Choi, Jong Rip Kim, Dae Hee Woo, Dong-Cheol Kim, Kyung Won Choi, Yoonseok Transl Oncol Original article Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties in conducting colorectal imaging. The purpose of this present study was to investigate the feasibility of using magnetic resonance colonography (MRC) to evaluate the efficacy of an ICI, an anti-PD-L1 antibody, in an orthotopic colorectal cancer mouse model. The mouse model was generated by the engraftment of colorectal cancer cells into the submucosal layer of the colon. Anti-cancer efficacy was assessed by tumor volume and metastatic tumor number analyses, and these values were significantly lower in the PD-L1 antibody-treated group compared to the controls. Histological analyses using H&E and Ki-67 immunohistochemical staining confirmed a highly efficacious tumor growth inhibition and enhanced infiltration by CD4+ and CD8+ lymphocytes in the PD-L1 antibody-treated group. We conclude that MRC has the potential to be used for ICI efficacy assessments against orthotopic colorectal cancer mouse model. Neoplasia Press 2019-07-11 /pmc/articles/PMC6626083/ /pubmed/31302474 http://dx.doi.org/10.1016/j.tranon.2019.06.006 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Chae, Yeon Ji
Kim, Jinil
Heo, Hwon
Woo, Chul-Woong
Kim, Sang-Tae
Kim, Min Jung
Choi, Jong Rip
Kim, Dae Hee
Woo, Dong-Cheol
Kim, Kyung Won
Choi, Yoonseok
Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title_full Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title_fullStr Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title_full_unstemmed Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title_short Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
title_sort magnetic resonance colonography enables the efficacy assessment of immune checkpoint inhibitors in an orthotopic colorectal cancer mouse model()()
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626083/
https://www.ncbi.nlm.nih.gov/pubmed/31302474
http://dx.doi.org/10.1016/j.tranon.2019.06.006
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