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Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()()
Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626083/ https://www.ncbi.nlm.nih.gov/pubmed/31302474 http://dx.doi.org/10.1016/j.tranon.2019.06.006 |
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author | Chae, Yeon Ji Kim, Jinil Heo, Hwon Woo, Chul-Woong Kim, Sang-Tae Kim, Min Jung Choi, Jong Rip Kim, Dae Hee Woo, Dong-Cheol Kim, Kyung Won Choi, Yoonseok |
author_facet | Chae, Yeon Ji Kim, Jinil Heo, Hwon Woo, Chul-Woong Kim, Sang-Tae Kim, Min Jung Choi, Jong Rip Kim, Dae Hee Woo, Dong-Cheol Kim, Kyung Won Choi, Yoonseok |
author_sort | Chae, Yeon Ji |
collection | PubMed |
description | Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties in conducting colorectal imaging. The purpose of this present study was to investigate the feasibility of using magnetic resonance colonography (MRC) to evaluate the efficacy of an ICI, an anti-PD-L1 antibody, in an orthotopic colorectal cancer mouse model. The mouse model was generated by the engraftment of colorectal cancer cells into the submucosal layer of the colon. Anti-cancer efficacy was assessed by tumor volume and metastatic tumor number analyses, and these values were significantly lower in the PD-L1 antibody-treated group compared to the controls. Histological analyses using H&E and Ki-67 immunohistochemical staining confirmed a highly efficacious tumor growth inhibition and enhanced infiltration by CD4+ and CD8+ lymphocytes in the PD-L1 antibody-treated group. We conclude that MRC has the potential to be used for ICI efficacy assessments against orthotopic colorectal cancer mouse model. |
format | Online Article Text |
id | pubmed-6626083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66260832019-07-23 Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() Chae, Yeon Ji Kim, Jinil Heo, Hwon Woo, Chul-Woong Kim, Sang-Tae Kim, Min Jung Choi, Jong Rip Kim, Dae Hee Woo, Dong-Cheol Kim, Kyung Won Choi, Yoonseok Transl Oncol Original article Immune checkpoint inhibitors (ICIs) have become an effective therapeutic option for colorectal cancer and studies on these drugs have therefore increased greatly. Efficacy assessments of ICIs in preclinical orthotopic colorectal cancer using MRI have not been reported however due to the difficulties in conducting colorectal imaging. The purpose of this present study was to investigate the feasibility of using magnetic resonance colonography (MRC) to evaluate the efficacy of an ICI, an anti-PD-L1 antibody, in an orthotopic colorectal cancer mouse model. The mouse model was generated by the engraftment of colorectal cancer cells into the submucosal layer of the colon. Anti-cancer efficacy was assessed by tumor volume and metastatic tumor number analyses, and these values were significantly lower in the PD-L1 antibody-treated group compared to the controls. Histological analyses using H&E and Ki-67 immunohistochemical staining confirmed a highly efficacious tumor growth inhibition and enhanced infiltration by CD4+ and CD8+ lymphocytes in the PD-L1 antibody-treated group. We conclude that MRC has the potential to be used for ICI efficacy assessments against orthotopic colorectal cancer mouse model. Neoplasia Press 2019-07-11 /pmc/articles/PMC6626083/ /pubmed/31302474 http://dx.doi.org/10.1016/j.tranon.2019.06.006 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Chae, Yeon Ji Kim, Jinil Heo, Hwon Woo, Chul-Woong Kim, Sang-Tae Kim, Min Jung Choi, Jong Rip Kim, Dae Hee Woo, Dong-Cheol Kim, Kyung Won Choi, Yoonseok Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title | Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title_full | Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title_fullStr | Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title_full_unstemmed | Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title_short | Magnetic Resonance Colonography Enables the Efficacy Assessment of Immune Checkpoint Inhibitors in an Orthotopic Colorectal Cancer Mouse Model()() |
title_sort | magnetic resonance colonography enables the efficacy assessment of immune checkpoint inhibitors in an orthotopic colorectal cancer mouse model()() |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626083/ https://www.ncbi.nlm.nih.gov/pubmed/31302474 http://dx.doi.org/10.1016/j.tranon.2019.06.006 |
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