Cargando…

SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation

BACKGROUND: Sterol-regulatory element binding protein 1 (SREBP1), an intracellular cholesterol sensor located in the endoplasmic reticulum, regulates the intracellular cholesterol by the Insig-Srebp-Scap pathway. Over-expression of SREBP1 can cause dyslipidemia. SREBP1 can regulate the metabolic pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Yuyan, Nan, Xianxiu, Shi, Xinjue, Mu, Xiaoqin, Liu, Binbin, Zhu, Huifen, Yao, Bingqing, Liu, Xinyi, Yang, Tianyue, Hu, Yiting, Liu, Shulin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626379/
https://www.ncbi.nlm.nih.gov/pubmed/31299935
http://dx.doi.org/10.1186/s12885-019-5904-x
_version_ 1783434565115707392
author Gao, Yuyan
Nan, Xianxiu
Shi, Xinjue
Mu, Xiaoqin
Liu, Binbin
Zhu, Huifen
Yao, Bingqing
Liu, Xinyi
Yang, Tianyue
Hu, Yiting
Liu, Shulin
author_facet Gao, Yuyan
Nan, Xianxiu
Shi, Xinjue
Mu, Xiaoqin
Liu, Binbin
Zhu, Huifen
Yao, Bingqing
Liu, Xinyi
Yang, Tianyue
Hu, Yiting
Liu, Shulin
author_sort Gao, Yuyan
collection PubMed
description BACKGROUND: Sterol-regulatory element binding protein 1 (SREBP1), an intracellular cholesterol sensor located in the endoplasmic reticulum, regulates the intracellular cholesterol by the Insig-Srebp-Scap pathway. Over-expression of SREBP1 can cause dyslipidemia. SREBP1 can regulate the metabolic pathway, and then promote the proliferation of tumor cells. However, there is no relevant research of metastasis and invasion in the field of colorectal cancer (CRC). METHODS: Expression of SREBP1 was manipulated in CRC cell lines with low and high level SREBP1 expression by transfectiong with plasmids containing the SREBP1 gene, or by shRNA. The effect of SREBP1 on cell migration was assayed. The expression of SREBP1, p65 and MMP7 were detected by western blot. Human umbilical vein endothelial cell was used for detection of angiogenesis by adding the culture supernatant from HT29 and SW620. The level of reactive oxygen species (ROS) was detected by Dihydroethidium (DHE) staining. NF-κB inhibitor SN50 was used to test the relationship of SREBP1, NF-κB pathway and MMP7. RESULTS: We found that the expression of SREBP1 in colon adenocarcinoma was significantly higher than that in noncancerous tissues, especially in the invasive tumor front including tumor budding. In vitro, SREBP1 over-expressed in colon cancer cell lines HT29 promoted angiogenesis in endothelial cells, increased ROS levels, phosphorylation of NF-κB-p65 and increases MMP7 expression. The effect of SREBP1 on expression of MMP7 was lost following treatment with the NF-κB inhibitor SN50. CONCLUSION: Our results suggest that SREBP1 can promote the invasion and metastasis of CRC cells by means of promoting the expression of MMP7 related to phosphorylation of p65. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-019-5904-x) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6626379
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-66263792019-07-23 SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation Gao, Yuyan Nan, Xianxiu Shi, Xinjue Mu, Xiaoqin Liu, Binbin Zhu, Huifen Yao, Bingqing Liu, Xinyi Yang, Tianyue Hu, Yiting Liu, Shulin BMC Cancer Research Article BACKGROUND: Sterol-regulatory element binding protein 1 (SREBP1), an intracellular cholesterol sensor located in the endoplasmic reticulum, regulates the intracellular cholesterol by the Insig-Srebp-Scap pathway. Over-expression of SREBP1 can cause dyslipidemia. SREBP1 can regulate the metabolic pathway, and then promote the proliferation of tumor cells. However, there is no relevant research of metastasis and invasion in the field of colorectal cancer (CRC). METHODS: Expression of SREBP1 was manipulated in CRC cell lines with low and high level SREBP1 expression by transfectiong with plasmids containing the SREBP1 gene, or by shRNA. The effect of SREBP1 on cell migration was assayed. The expression of SREBP1, p65 and MMP7 were detected by western blot. Human umbilical vein endothelial cell was used for detection of angiogenesis by adding the culture supernatant from HT29 and SW620. The level of reactive oxygen species (ROS) was detected by Dihydroethidium (DHE) staining. NF-κB inhibitor SN50 was used to test the relationship of SREBP1, NF-κB pathway and MMP7. RESULTS: We found that the expression of SREBP1 in colon adenocarcinoma was significantly higher than that in noncancerous tissues, especially in the invasive tumor front including tumor budding. In vitro, SREBP1 over-expressed in colon cancer cell lines HT29 promoted angiogenesis in endothelial cells, increased ROS levels, phosphorylation of NF-κB-p65 and increases MMP7 expression. The effect of SREBP1 on expression of MMP7 was lost following treatment with the NF-κB inhibitor SN50. CONCLUSION: Our results suggest that SREBP1 can promote the invasion and metastasis of CRC cells by means of promoting the expression of MMP7 related to phosphorylation of p65. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-019-5904-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-12 /pmc/articles/PMC6626379/ /pubmed/31299935 http://dx.doi.org/10.1186/s12885-019-5904-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gao, Yuyan
Nan, Xianxiu
Shi, Xinjue
Mu, Xiaoqin
Liu, Binbin
Zhu, Huifen
Yao, Bingqing
Liu, Xinyi
Yang, Tianyue
Hu, Yiting
Liu, Shulin
SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title_full SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title_fullStr SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title_full_unstemmed SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title_short SREBP1 promotes the invasion of colorectal cancer accompanied upregulation of MMP7 expression and NF-κB pathway activation
title_sort srebp1 promotes the invasion of colorectal cancer accompanied upregulation of mmp7 expression and nf-κb pathway activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626379/
https://www.ncbi.nlm.nih.gov/pubmed/31299935
http://dx.doi.org/10.1186/s12885-019-5904-x
work_keys_str_mv AT gaoyuyan srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT nanxianxiu srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT shixinjue srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT muxiaoqin srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT liubinbin srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT zhuhuifen srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT yaobingqing srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT liuxinyi srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT yangtianyue srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT huyiting srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation
AT liushulin srebp1promotestheinvasionofcolorectalcanceraccompaniedupregulationofmmp7expressionandnfkbpathwayactivation