Increasing the specificity of CRISPR systems with engineered RNA secondary structures

CRISPR systems have been broadly adopted for basic science, biotechnology, and gene and cell therapy. In some cases, these bacterial nucleases have demonstrated off-target activity. This creates a potential hazard for therapeutic applications and could confound results in biological research. Theref...

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Detalles Bibliográficos
Autores principales: Kocak, D. Dewran, Josephs, Eric A., Bhandarkar, Vidit, Adkar, Shaunak S., Kwon, Jennifer B., Gersbach, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626619/
https://www.ncbi.nlm.nih.gov/pubmed/30988504
http://dx.doi.org/10.1038/s41587-019-0095-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626619/
https://www.ncbi.nlm.nih.gov/pubmed/30988504
http://dx.doi.org/10.1038/s41587-019-0095-1

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