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Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury
Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracin...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626851/ https://www.ncbi.nlm.nih.gov/pubmed/31204300 http://dx.doi.org/10.1016/j.stemcr.2019.05.011 |
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author | Lu, Paul Gomes-Leal, Walace Anil, Selin Dobkins, Gabriel Huie, J. Russell Ferguson, Adam R. Graham, Lori Tuszynski, Mark |
author_facet | Lu, Paul Gomes-Leal, Walace Anil, Selin Dobkins, Gabriel Huie, J. Russell Ferguson, Adam R. Graham, Lori Tuszynski, Mark |
author_sort | Lu, Paul |
collection | PubMed |
description | Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracing into floxed-TdTomato-expressing NPC grafts. 7,640 ± 630 grafted neurons extended axons to a single caudal host spinal cord site located 2 mm beyond the lesion, 5 weeks post injury. While caudally projecting axons arose from neurons located in all regions of the graft, the majority of caudally projecting graft neurons (53%) were located within the caudal one-third of the graft. Numerous host corticospinal axons formed monosynaptic projections onto caudally projecting graft neurons; however, we find that the majority of host axonal neuronal projections formed by neural progenitor cell interneuronal “relays” across sites of SCI are likely polysynaptic in nature. |
format | Online Article Text |
id | pubmed-6626851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-66268512019-07-23 Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury Lu, Paul Gomes-Leal, Walace Anil, Selin Dobkins, Gabriel Huie, J. Russell Ferguson, Adam R. Graham, Lori Tuszynski, Mark Stem Cell Reports Article Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracing into floxed-TdTomato-expressing NPC grafts. 7,640 ± 630 grafted neurons extended axons to a single caudal host spinal cord site located 2 mm beyond the lesion, 5 weeks post injury. While caudally projecting axons arose from neurons located in all regions of the graft, the majority of caudally projecting graft neurons (53%) were located within the caudal one-third of the graft. Numerous host corticospinal axons formed monosynaptic projections onto caudally projecting graft neurons; however, we find that the majority of host axonal neuronal projections formed by neural progenitor cell interneuronal “relays” across sites of SCI are likely polysynaptic in nature. Elsevier 2019-06-13 /pmc/articles/PMC6626851/ /pubmed/31204300 http://dx.doi.org/10.1016/j.stemcr.2019.05.011 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Lu, Paul Gomes-Leal, Walace Anil, Selin Dobkins, Gabriel Huie, J. Russell Ferguson, Adam R. Graham, Lori Tuszynski, Mark Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title | Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title_full | Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title_fullStr | Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title_full_unstemmed | Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title_short | Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury |
title_sort | origins of neural progenitor cell-derived axons projecting caudally after spinal cord injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626851/ https://www.ncbi.nlm.nih.gov/pubmed/31204300 http://dx.doi.org/10.1016/j.stemcr.2019.05.011 |
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