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Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus w...

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Autores principales: Wang, Yi, Lan, Changjun, Liao, Xuan, Chen, Di, Song, Wengang, Zhang, Qiuling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626950/
https://www.ncbi.nlm.nih.gov/pubmed/30426692
http://dx.doi.org/10.1111/jdi.12976
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author Wang, Yi
Lan, Changjun
Liao, Xuan
Chen, Di
Song, Wengang
Zhang, Qiuling
author_facet Wang, Yi
Lan, Changjun
Liao, Xuan
Chen, Di
Song, Wengang
Zhang, Qiuling
author_sort Wang, Yi
collection PubMed
description AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus with PSP intervention of high, medium and low doses groups. The difference of retinal vascularization between groups was evaluated by fluorescein isothiocyanate‐dextran perfusion. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was used to assess apoptosis in the retinal ganglion cells; reverse transcriptase polymerase chain reaction and western blotting were utilized to evaluate the expression of Bcl2‐associated X protein, B‐cell lymphoma‐2 factor, epidermal growth factor, p38 mitogen‐activated protein kinases, transforming growth factor‐β and vascular endothelial growth factor at the messenger ribonucleic acid and protein level. RESULTS: Fluorescein isothiocyanate‐dextran perfusion showed retinal vascular anomaly in diabetes mellitus rats, but vascular tortuosity and leakage were relatively alleviated after PSP intervention. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed numerous terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling‐positive retinal cells in the diabetes mellitus group, which then were reduced by PSP treatment. Reverse transcriptase polymerase chain reaction showed that PSP intervention decreased Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β messenger ribonucleic acid expression, but increased B‐cell lymphoma‐2 factor messenger ribonucleic acid expression. Western blot showed that PSP intervention upregulated the expression of B‐cell lymphoma‐2 factor, and downregulated the expression of Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β proteins. CONCLUSIONS: Polygonatum sibiricum polysaccharide shows a protective effect against diabetes‐induced retinal injury in a dose‐dependent manner. The mechanism of action deserves further study and exploration.
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spelling pubmed-66269502019-07-17 Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model Wang, Yi Lan, Changjun Liao, Xuan Chen, Di Song, Wengang Zhang, Qiuling J Diabetes Investig Articles AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus with PSP intervention of high, medium and low doses groups. The difference of retinal vascularization between groups was evaluated by fluorescein isothiocyanate‐dextran perfusion. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was used to assess apoptosis in the retinal ganglion cells; reverse transcriptase polymerase chain reaction and western blotting were utilized to evaluate the expression of Bcl2‐associated X protein, B‐cell lymphoma‐2 factor, epidermal growth factor, p38 mitogen‐activated protein kinases, transforming growth factor‐β and vascular endothelial growth factor at the messenger ribonucleic acid and protein level. RESULTS: Fluorescein isothiocyanate‐dextran perfusion showed retinal vascular anomaly in diabetes mellitus rats, but vascular tortuosity and leakage were relatively alleviated after PSP intervention. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed numerous terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling‐positive retinal cells in the diabetes mellitus group, which then were reduced by PSP treatment. Reverse transcriptase polymerase chain reaction showed that PSP intervention decreased Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β messenger ribonucleic acid expression, but increased B‐cell lymphoma‐2 factor messenger ribonucleic acid expression. Western blot showed that PSP intervention upregulated the expression of B‐cell lymphoma‐2 factor, and downregulated the expression of Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β proteins. CONCLUSIONS: Polygonatum sibiricum polysaccharide shows a protective effect against diabetes‐induced retinal injury in a dose‐dependent manner. The mechanism of action deserves further study and exploration. John Wiley and Sons Inc. 2018-12-16 2019-07 /pmc/articles/PMC6626950/ /pubmed/30426692 http://dx.doi.org/10.1111/jdi.12976 Text en © 2018 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Articles
Wang, Yi
Lan, Changjun
Liao, Xuan
Chen, Di
Song, Wengang
Zhang, Qiuling
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title_full Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title_fullStr Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title_full_unstemmed Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title_short Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
title_sort polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626950/
https://www.ncbi.nlm.nih.gov/pubmed/30426692
http://dx.doi.org/10.1111/jdi.12976
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