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Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model
AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626950/ https://www.ncbi.nlm.nih.gov/pubmed/30426692 http://dx.doi.org/10.1111/jdi.12976 |
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author | Wang, Yi Lan, Changjun Liao, Xuan Chen, Di Song, Wengang Zhang, Qiuling |
author_facet | Wang, Yi Lan, Changjun Liao, Xuan Chen, Di Song, Wengang Zhang, Qiuling |
author_sort | Wang, Yi |
collection | PubMed |
description | AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus with PSP intervention of high, medium and low doses groups. The difference of retinal vascularization between groups was evaluated by fluorescein isothiocyanate‐dextran perfusion. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was used to assess apoptosis in the retinal ganglion cells; reverse transcriptase polymerase chain reaction and western blotting were utilized to evaluate the expression of Bcl2‐associated X protein, B‐cell lymphoma‐2 factor, epidermal growth factor, p38 mitogen‐activated protein kinases, transforming growth factor‐β and vascular endothelial growth factor at the messenger ribonucleic acid and protein level. RESULTS: Fluorescein isothiocyanate‐dextran perfusion showed retinal vascular anomaly in diabetes mellitus rats, but vascular tortuosity and leakage were relatively alleviated after PSP intervention. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed numerous terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling‐positive retinal cells in the diabetes mellitus group, which then were reduced by PSP treatment. Reverse transcriptase polymerase chain reaction showed that PSP intervention decreased Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β messenger ribonucleic acid expression, but increased B‐cell lymphoma‐2 factor messenger ribonucleic acid expression. Western blot showed that PSP intervention upregulated the expression of B‐cell lymphoma‐2 factor, and downregulated the expression of Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β proteins. CONCLUSIONS: Polygonatum sibiricum polysaccharide shows a protective effect against diabetes‐induced retinal injury in a dose‐dependent manner. The mechanism of action deserves further study and exploration. |
format | Online Article Text |
id | pubmed-6626950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66269502019-07-17 Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model Wang, Yi Lan, Changjun Liao, Xuan Chen, Di Song, Wengang Zhang, Qiuling J Diabetes Investig Articles AIMS/INTRODUCTION: To investigate the protective effect of Polygonatum sibiricum polysaccharide (PSP) on the retina in diabetic rats. MATERIALS AND METHODS: A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus with PSP intervention of high, medium and low doses groups. The difference of retinal vascularization between groups was evaluated by fluorescein isothiocyanate‐dextran perfusion. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was used to assess apoptosis in the retinal ganglion cells; reverse transcriptase polymerase chain reaction and western blotting were utilized to evaluate the expression of Bcl2‐associated X protein, B‐cell lymphoma‐2 factor, epidermal growth factor, p38 mitogen‐activated protein kinases, transforming growth factor‐β and vascular endothelial growth factor at the messenger ribonucleic acid and protein level. RESULTS: Fluorescein isothiocyanate‐dextran perfusion showed retinal vascular anomaly in diabetes mellitus rats, but vascular tortuosity and leakage were relatively alleviated after PSP intervention. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed numerous terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling‐positive retinal cells in the diabetes mellitus group, which then were reduced by PSP treatment. Reverse transcriptase polymerase chain reaction showed that PSP intervention decreased Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β messenger ribonucleic acid expression, but increased B‐cell lymphoma‐2 factor messenger ribonucleic acid expression. Western blot showed that PSP intervention upregulated the expression of B‐cell lymphoma‐2 factor, and downregulated the expression of Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β proteins. CONCLUSIONS: Polygonatum sibiricum polysaccharide shows a protective effect against diabetes‐induced retinal injury in a dose‐dependent manner. The mechanism of action deserves further study and exploration. John Wiley and Sons Inc. 2018-12-16 2019-07 /pmc/articles/PMC6626950/ /pubmed/30426692 http://dx.doi.org/10.1111/jdi.12976 Text en © 2018 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Articles Wang, Yi Lan, Changjun Liao, Xuan Chen, Di Song, Wengang Zhang, Qiuling Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title |
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title_full |
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title_fullStr |
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title_full_unstemmed |
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title_short |
Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
title_sort | polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626950/ https://www.ncbi.nlm.nih.gov/pubmed/30426692 http://dx.doi.org/10.1111/jdi.12976 |
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