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RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo
Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jingha...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627390/ https://www.ncbi.nlm.nih.gov/pubmed/31159150 http://dx.doi.org/10.3390/genes10060420 |
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author | Wang, Xiaohui Zou, Wenbin Yu, Hailiang Lin, Yuxin Dai, Guojun Zhang, Tao Zhang, Genxi Xie, Kaizhou Wang, Jinyu Shi, Huiqiang |
author_facet | Wang, Xiaohui Zou, Wenbin Yu, Hailiang Lin, Yuxin Dai, Guojun Zhang, Tao Zhang, Genxi Xie, Kaizhou Wang, Jinyu Shi, Huiqiang |
author_sort | Wang, Xiaohui |
collection | PubMed |
description | Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jinghai yellow chickens infected with E. tenella, the molecular response process, and the immune response mechanism during coccidial infection, RNA-seq was used to analyze the cecal tissues of an E. tenella infection group (JS) and an uninfected group (JC) on the seventh day post-infection. The DEGs were screened by functional and pathway enrichment analyses. The results indicated that there were 5477 DEGs (p-value < 0.05) between the JS and the JC groups, of which 2942 were upregulated, and 2535 were downregulated. GO analysis indicated that the top 30 significantly enriched GO terms mainly involved signal transduction, angiogenesis, inflammatory response, and blood vessel development. KEGG analysis revealed that the top significantly enriched signaling pathways included focal adhesion, extracellular matrix–receptor interaction, and peroxisome proliferator-activated receptor. The key DEGs in these pathways included ANGPTL4, ACSL5, VEGFC, MAPK10, and CD44. These genes play an important role in the infection of E. tenella. This study further enhances our understanding of the molecular mechanism of E. tenella infection in chickens. |
format | Online Article Text |
id | pubmed-6627390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66273902019-07-23 RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo Wang, Xiaohui Zou, Wenbin Yu, Hailiang Lin, Yuxin Dai, Guojun Zhang, Tao Zhang, Genxi Xie, Kaizhou Wang, Jinyu Shi, Huiqiang Genes (Basel) Article Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jinghai yellow chickens infected with E. tenella, the molecular response process, and the immune response mechanism during coccidial infection, RNA-seq was used to analyze the cecal tissues of an E. tenella infection group (JS) and an uninfected group (JC) on the seventh day post-infection. The DEGs were screened by functional and pathway enrichment analyses. The results indicated that there were 5477 DEGs (p-value < 0.05) between the JS and the JC groups, of which 2942 were upregulated, and 2535 were downregulated. GO analysis indicated that the top 30 significantly enriched GO terms mainly involved signal transduction, angiogenesis, inflammatory response, and blood vessel development. KEGG analysis revealed that the top significantly enriched signaling pathways included focal adhesion, extracellular matrix–receptor interaction, and peroxisome proliferator-activated receptor. The key DEGs in these pathways included ANGPTL4, ACSL5, VEGFC, MAPK10, and CD44. These genes play an important role in the infection of E. tenella. This study further enhances our understanding of the molecular mechanism of E. tenella infection in chickens. MDPI 2019-05-31 /pmc/articles/PMC6627390/ /pubmed/31159150 http://dx.doi.org/10.3390/genes10060420 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xiaohui Zou, Wenbin Yu, Hailiang Lin, Yuxin Dai, Guojun Zhang, Tao Zhang, Genxi Xie, Kaizhou Wang, Jinyu Shi, Huiqiang RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title | RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title_full | RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title_fullStr | RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title_full_unstemmed | RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title_short | RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo |
title_sort | rna sequencing analysis of chicken cecum tissues following eimeria tenella infection in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627390/ https://www.ncbi.nlm.nih.gov/pubmed/31159150 http://dx.doi.org/10.3390/genes10060420 |
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