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Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B
Introduction: In our previous study, we constructed a Lung Cancer Explorer (LCE) database housing lung cancer-specific expression data and clinical data from over 6700 patients in 56 studies. Methods: Using this dataset of the largest collection of lung cancer gene expression along with our meta-ana...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627508/ https://www.ncbi.nlm.nih.gov/pubmed/31242643 http://dx.doi.org/10.3390/cancers11060886 |
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author | Cai, Ling Luo, Danni Yao, Bo Yang, Donghan M. Lin, ShinYi Girard, Luc DeBerardinis, Ralph J. Minna, John D. Xie, Yang Xiao, Guanghua |
author_facet | Cai, Ling Luo, Danni Yao, Bo Yang, Donghan M. Lin, ShinYi Girard, Luc DeBerardinis, Ralph J. Minna, John D. Xie, Yang Xiao, Guanghua |
author_sort | Cai, Ling |
collection | PubMed |
description | Introduction: In our previous study, we constructed a Lung Cancer Explorer (LCE) database housing lung cancer-specific expression data and clinical data from over 6700 patients in 56 studies. Methods: Using this dataset of the largest collection of lung cancer gene expression along with our meta-analysis method, we systematically interrogated the association between gene expression and overall survival as well as the expression difference between tumor and normal (adjacent non-malignant tissue) samples in lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SQCC). A case study for FAM83A and FAM83B was performed as a demonstration for hypothesis testing with our database. Results: We showed that the reproducibility of results across studies varied by histological subtype and analysis type. Genes and pathways unique or common to the two histological subtypes were identified and the results were integrated into LCE to facilitate user exploration. In our case study, we verified the findings from a previous study on FAM83A and FAM83B in non-small cell lung cancer. Conclusions: This study used gene expression data from a large cohort of patients to explore the molecular differences between lung ADC and SQCC. |
format | Online Article Text |
id | pubmed-6627508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66275082019-07-23 Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B Cai, Ling Luo, Danni Yao, Bo Yang, Donghan M. Lin, ShinYi Girard, Luc DeBerardinis, Ralph J. Minna, John D. Xie, Yang Xiao, Guanghua Cancers (Basel) Article Introduction: In our previous study, we constructed a Lung Cancer Explorer (LCE) database housing lung cancer-specific expression data and clinical data from over 6700 patients in 56 studies. Methods: Using this dataset of the largest collection of lung cancer gene expression along with our meta-analysis method, we systematically interrogated the association between gene expression and overall survival as well as the expression difference between tumor and normal (adjacent non-malignant tissue) samples in lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SQCC). A case study for FAM83A and FAM83B was performed as a demonstration for hypothesis testing with our database. Results: We showed that the reproducibility of results across studies varied by histological subtype and analysis type. Genes and pathways unique or common to the two histological subtypes were identified and the results were integrated into LCE to facilitate user exploration. In our case study, we verified the findings from a previous study on FAM83A and FAM83B in non-small cell lung cancer. Conclusions: This study used gene expression data from a large cohort of patients to explore the molecular differences between lung ADC and SQCC. MDPI 2019-06-25 /pmc/articles/PMC6627508/ /pubmed/31242643 http://dx.doi.org/10.3390/cancers11060886 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Ling Luo, Danni Yao, Bo Yang, Donghan M. Lin, ShinYi Girard, Luc DeBerardinis, Ralph J. Minna, John D. Xie, Yang Xiao, Guanghua Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title | Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title_full | Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title_fullStr | Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title_full_unstemmed | Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title_short | Systematic Analysis of Gene Expression in Lung Adenocarcinoma and Squamous Cell Carcinoma with a Case Study of FAM83A and FAM83B |
title_sort | systematic analysis of gene expression in lung adenocarcinoma and squamous cell carcinoma with a case study of fam83a and fam83b |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627508/ https://www.ncbi.nlm.nih.gov/pubmed/31242643 http://dx.doi.org/10.3390/cancers11060886 |
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