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Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles

Pancreatic cancer is a concealed and highly malignant tumor, and its early diagnosis plays an increasingly weighty role during the course of cancer treatment. In this study, we developed a polymeric magnetic resonance imaging (MRI) nanoplatform for MRI contrast agents. To improve tumor-targeting del...

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Autores principales: Zhu, Xiuliang, Lu, Nan, Zhou, Ying, Xuan, Shaoyan, Zhang, Jiaojiao, Giampieri, Francesca, Zhang, Yongping, Yang, Fangfang, Yu, Risheng, Battino, Maurizio, Wang, Zuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627612/
https://www.ncbi.nlm.nih.gov/pubmed/31248076
http://dx.doi.org/10.3390/ijms20122988
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author Zhu, Xiuliang
Lu, Nan
Zhou, Ying
Xuan, Shaoyan
Zhang, Jiaojiao
Giampieri, Francesca
Zhang, Yongping
Yang, Fangfang
Yu, Risheng
Battino, Maurizio
Wang, Zuhua
author_facet Zhu, Xiuliang
Lu, Nan
Zhou, Ying
Xuan, Shaoyan
Zhang, Jiaojiao
Giampieri, Francesca
Zhang, Yongping
Yang, Fangfang
Yu, Risheng
Battino, Maurizio
Wang, Zuhua
author_sort Zhu, Xiuliang
collection PubMed
description Pancreatic cancer is a concealed and highly malignant tumor, and its early diagnosis plays an increasingly weighty role during the course of cancer treatment. In this study, we developed a polymeric magnetic resonance imaging (MRI) nanoplatform for MRI contrast agents. To improve tumor-targeting delivery of MRI contrast agents, we employed a pancreatic cancer targeting CKAAKN peptide to prepare a peptide-functionalized amphiphilic hyaluronic acid–vitamin E succinate polymer (CKAAKN–HA–VES) for delivering ultra-small superparamagnetic iron oxide (USPIO), namely, CKAAKN–HA–VES@USPIO. With the modification of the CKAAKN peptide, CKAAKN–HA–VES@USPIO could specifically internalize into CKAAKN-positive BxPC-3 cells. The CKAAKN–HA–VES@USPIO nanoparticles presented a more specific accumulation into pancreatic cancer cells than normal pancreatic cells, and an obvious decrease in signal intensity was observed in CKAAKN-positive BxPC-3 cells, compared with CKAAKN-negative HPDE6-C7 cells and non-targeting HA–VES@USPIO nanoparticles. The results demonstrated that our polymeric MRI nanoplatform could selectively internalize into CKAAKN-positive pancreatic cancer cells by the specific binding of CKAAKN peptide with pancreatic cancer cell membrane receptors, which provided a novel polymeric MRI contrast agent with high specificity for pancreatic cancer diagnosis, and makes it a very promising candidate for magnetic resonance imaging contrast enhancement.
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spelling pubmed-66276122019-07-23 Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles Zhu, Xiuliang Lu, Nan Zhou, Ying Xuan, Shaoyan Zhang, Jiaojiao Giampieri, Francesca Zhang, Yongping Yang, Fangfang Yu, Risheng Battino, Maurizio Wang, Zuhua Int J Mol Sci Article Pancreatic cancer is a concealed and highly malignant tumor, and its early diagnosis plays an increasingly weighty role during the course of cancer treatment. In this study, we developed a polymeric magnetic resonance imaging (MRI) nanoplatform for MRI contrast agents. To improve tumor-targeting delivery of MRI contrast agents, we employed a pancreatic cancer targeting CKAAKN peptide to prepare a peptide-functionalized amphiphilic hyaluronic acid–vitamin E succinate polymer (CKAAKN–HA–VES) for delivering ultra-small superparamagnetic iron oxide (USPIO), namely, CKAAKN–HA–VES@USPIO. With the modification of the CKAAKN peptide, CKAAKN–HA–VES@USPIO could specifically internalize into CKAAKN-positive BxPC-3 cells. The CKAAKN–HA–VES@USPIO nanoparticles presented a more specific accumulation into pancreatic cancer cells than normal pancreatic cells, and an obvious decrease in signal intensity was observed in CKAAKN-positive BxPC-3 cells, compared with CKAAKN-negative HPDE6-C7 cells and non-targeting HA–VES@USPIO nanoparticles. The results demonstrated that our polymeric MRI nanoplatform could selectively internalize into CKAAKN-positive pancreatic cancer cells by the specific binding of CKAAKN peptide with pancreatic cancer cell membrane receptors, which provided a novel polymeric MRI contrast agent with high specificity for pancreatic cancer diagnosis, and makes it a very promising candidate for magnetic resonance imaging contrast enhancement. MDPI 2019-06-19 /pmc/articles/PMC6627612/ /pubmed/31248076 http://dx.doi.org/10.3390/ijms20122988 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Xiuliang
Lu, Nan
Zhou, Ying
Xuan, Shaoyan
Zhang, Jiaojiao
Giampieri, Francesca
Zhang, Yongping
Yang, Fangfang
Yu, Risheng
Battino, Maurizio
Wang, Zuhua
Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title_full Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title_fullStr Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title_full_unstemmed Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title_short Targeting Pancreatic Cancer Cells with Peptide-Functionalized Polymeric Magnetic Nanoparticles
title_sort targeting pancreatic cancer cells with peptide-functionalized polymeric magnetic nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627612/
https://www.ncbi.nlm.nih.gov/pubmed/31248076
http://dx.doi.org/10.3390/ijms20122988
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