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Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride

Vinyl chloride (VC) is a noninfective occupational risk factor. It is found in industrial chemicals, volatile organic compounds, cigarette smoke ingredients, etc. It is a kind of toxic gas that causes many diseases. VC exposure causes an increased risk of liver fibrosis and can result in angiosarcom...

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Autores principales: Hsu, Yung-Ho, Chuang, Hsiao-Chi, Lee, Yu-Hsuan, Lin, Yuh-Feng, Chiu, Yu-Jhe, Wang, Yung-Li, Wu, Mai-Szu, Chiu, Hui-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627785/
https://www.ncbi.nlm.nih.gov/pubmed/31212930
http://dx.doi.org/10.3390/cells8060601
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author Hsu, Yung-Ho
Chuang, Hsiao-Chi
Lee, Yu-Hsuan
Lin, Yuh-Feng
Chiu, Yu-Jhe
Wang, Yung-Li
Wu, Mai-Szu
Chiu, Hui-Wen
author_facet Hsu, Yung-Ho
Chuang, Hsiao-Chi
Lee, Yu-Hsuan
Lin, Yuh-Feng
Chiu, Yu-Jhe
Wang, Yung-Li
Wu, Mai-Szu
Chiu, Hui-Wen
author_sort Hsu, Yung-Ho
collection PubMed
description Vinyl chloride (VC) is a noninfective occupational risk factor. It is found in industrial chemicals, volatile organic compounds, cigarette smoke ingredients, etc. It is a kind of toxic gas that causes many diseases. VC exposure causes an increased risk of liver fibrosis and can result in angiosarcoma of the liver. Previous studies have shown that high-doses of VC exposure in mice resulted in acute death with marked tubular necrosis of the renal cortex. In this study, we assessed the nephrotoxicity of VC in vitro and in vivo. As a result, we demonstrated that VC induced fibrosis-associated protein expression, such as connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI-1) and collagen 1, and autophagy-associated protein expression, such as Beclin 1 and LC3-II, in kidney cells. The beclin1 siRNA experiments found that autophagy inhibited VC-induced fibrosis. Blood urea nitrogen (BUN) and creatinine levels were increased after VC treatment. Furthermore, VC caused glomerulosclerosis and tubular injury in mouse kidney tissues. Kidney tissue sections showed that VC induced fibrosis and autophagy in mouse kidney tissues. In summary, the results of VC-induced fibrosis suggest that autophagy plays an important role in kidney damage. VC may cause nephrotoxicity, and the results illustrate the importance of considering the toxicological hazards of VC in kidney cells.
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spelling pubmed-66277852019-07-23 Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride Hsu, Yung-Ho Chuang, Hsiao-Chi Lee, Yu-Hsuan Lin, Yuh-Feng Chiu, Yu-Jhe Wang, Yung-Li Wu, Mai-Szu Chiu, Hui-Wen Cells Article Vinyl chloride (VC) is a noninfective occupational risk factor. It is found in industrial chemicals, volatile organic compounds, cigarette smoke ingredients, etc. It is a kind of toxic gas that causes many diseases. VC exposure causes an increased risk of liver fibrosis and can result in angiosarcoma of the liver. Previous studies have shown that high-doses of VC exposure in mice resulted in acute death with marked tubular necrosis of the renal cortex. In this study, we assessed the nephrotoxicity of VC in vitro and in vivo. As a result, we demonstrated that VC induced fibrosis-associated protein expression, such as connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI-1) and collagen 1, and autophagy-associated protein expression, such as Beclin 1 and LC3-II, in kidney cells. The beclin1 siRNA experiments found that autophagy inhibited VC-induced fibrosis. Blood urea nitrogen (BUN) and creatinine levels were increased after VC treatment. Furthermore, VC caused glomerulosclerosis and tubular injury in mouse kidney tissues. Kidney tissue sections showed that VC induced fibrosis and autophagy in mouse kidney tissues. In summary, the results of VC-induced fibrosis suggest that autophagy plays an important role in kidney damage. VC may cause nephrotoxicity, and the results illustrate the importance of considering the toxicological hazards of VC in kidney cells. MDPI 2019-06-17 /pmc/articles/PMC6627785/ /pubmed/31212930 http://dx.doi.org/10.3390/cells8060601 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hsu, Yung-Ho
Chuang, Hsiao-Chi
Lee, Yu-Hsuan
Lin, Yuh-Feng
Chiu, Yu-Jhe
Wang, Yung-Li
Wu, Mai-Szu
Chiu, Hui-Wen
Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title_full Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title_fullStr Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title_full_unstemmed Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title_short Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride
title_sort induction of fibrosis and autophagy in kidney cells by vinyl chloride
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627785/
https://www.ncbi.nlm.nih.gov/pubmed/31212930
http://dx.doi.org/10.3390/cells8060601
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