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Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales

“Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. U...

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Autores principales: Golyshina, Olga V., Bargiela, Rafael, Toshchakov, Stepan V., Chernyh, Nikolay A., Ramayah, Soshila, Korzhenkov, Aleksei A., Kublanov, Ilya V., Golyshin, Peter N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627985/
https://www.ncbi.nlm.nih.gov/pubmed/31208064
http://dx.doi.org/10.3390/genes10060461
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author Golyshina, Olga V.
Bargiela, Rafael
Toshchakov, Stepan V.
Chernyh, Nikolay A.
Ramayah, Soshila
Korzhenkov, Aleksei A.
Kublanov, Ilya V.
Golyshin, Peter N.
author_facet Golyshina, Olga V.
Bargiela, Rafael
Toshchakov, Stepan V.
Chernyh, Nikolay A.
Ramayah, Soshila
Korzhenkov, Aleksei A.
Kublanov, Ilya V.
Golyshin, Peter N.
author_sort Golyshina, Olga V.
collection PubMed
description “Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “Ca. Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “Ca. Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “Ca. Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “Ca. Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “Ca. Micrarchaeota” and certain Thermoplasmatales, such as Cuniculiplasma spp. in situ.
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spelling pubmed-66279852019-07-23 Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales Golyshina, Olga V. Bargiela, Rafael Toshchakov, Stepan V. Chernyh, Nikolay A. Ramayah, Soshila Korzhenkov, Aleksei A. Kublanov, Ilya V. Golyshin, Peter N. Genes (Basel) Article “Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “Ca. Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “Ca. Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “Ca. Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “Ca. Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “Ca. Micrarchaeota” and certain Thermoplasmatales, such as Cuniculiplasma spp. in situ. MDPI 2019-06-15 /pmc/articles/PMC6627985/ /pubmed/31208064 http://dx.doi.org/10.3390/genes10060461 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Golyshina, Olga V.
Bargiela, Rafael
Toshchakov, Stepan V.
Chernyh, Nikolay A.
Ramayah, Soshila
Korzhenkov, Aleksei A.
Kublanov, Ilya V.
Golyshin, Peter N.
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title_full Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title_fullStr Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title_full_unstemmed Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title_short Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
title_sort diversity of “ca. micrarchaeota” in two distinct types of acidic environments and their associations with thermoplasmatales
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627985/
https://www.ncbi.nlm.nih.gov/pubmed/31208064
http://dx.doi.org/10.3390/genes10060461
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