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Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions
A biosensitive element in the form of bacterial Photobacterium phosphoreum cells immobilized in poly(vinyl alcohol) cryogel was tested for the determination of different mycotoxins under discrete and flow-through analysis conditions. The immobilized bioluminescent cells made it possible to quantify...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627987/ https://www.ncbi.nlm.nih.gov/pubmed/31137498 http://dx.doi.org/10.3390/bios9020063 |
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author | Senko, Olga Stepanov, Nikolay Maslova, Olga Akhundov, Rashid Ismailov, Anvar Efremenko, Elena |
author_facet | Senko, Olga Stepanov, Nikolay Maslova, Olga Akhundov, Rashid Ismailov, Anvar Efremenko, Elena |
author_sort | Senko, Olga |
collection | PubMed |
description | A biosensitive element in the form of bacterial Photobacterium phosphoreum cells immobilized in poly(vinyl alcohol) cryogel was tested for the determination of different mycotoxins under discrete and flow-through analysis conditions. The immobilized bioluminescent cells made it possible to quantify the presence of Ochratoxin A, Sterigmatocystin, Zearalenone, and Deoxynivalenon in aqueous media in a wide range of their concentrations (0.017–56 mg/L, 0.010–33 mg/L, 0.009–14 mg/L, and 0.026–177 mg/L, respectively) via measuring the quenching of cell luminescence. The flow conditions allowed the analysis sensitivity to be improved by an order of magnitude in terms of detected concentrations. Using the immobilized luminescent bacterial cells, we have shown the possibility of evaluating the efficiency of the mycotoxins’ hydrolysis under the action of enzymes. In this way, a 94 ± 4.5% efficiency of Zearalenone hydrolysis with hexahistidine-containing organophosphorus hydrolase for 1h-long treatment of the mycotoxin solution (100 mg/L) was shown. |
format | Online Article Text |
id | pubmed-6627987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66279872019-07-23 Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions Senko, Olga Stepanov, Nikolay Maslova, Olga Akhundov, Rashid Ismailov, Anvar Efremenko, Elena Biosensors (Basel) Article A biosensitive element in the form of bacterial Photobacterium phosphoreum cells immobilized in poly(vinyl alcohol) cryogel was tested for the determination of different mycotoxins under discrete and flow-through analysis conditions. The immobilized bioluminescent cells made it possible to quantify the presence of Ochratoxin A, Sterigmatocystin, Zearalenone, and Deoxynivalenon in aqueous media in a wide range of their concentrations (0.017–56 mg/L, 0.010–33 mg/L, 0.009–14 mg/L, and 0.026–177 mg/L, respectively) via measuring the quenching of cell luminescence. The flow conditions allowed the analysis sensitivity to be improved by an order of magnitude in terms of detected concentrations. Using the immobilized luminescent bacterial cells, we have shown the possibility of evaluating the efficiency of the mycotoxins’ hydrolysis under the action of enzymes. In this way, a 94 ± 4.5% efficiency of Zearalenone hydrolysis with hexahistidine-containing organophosphorus hydrolase for 1h-long treatment of the mycotoxin solution (100 mg/L) was shown. MDPI 2019-05-20 /pmc/articles/PMC6627987/ /pubmed/31137498 http://dx.doi.org/10.3390/bios9020063 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Senko, Olga Stepanov, Nikolay Maslova, Olga Akhundov, Rashid Ismailov, Anvar Efremenko, Elena Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title | Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title_full | Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title_fullStr | Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title_full_unstemmed | Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title_short | Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions |
title_sort | immobilized luminescent bacteria for the detection of mycotoxins under discrete and flow-through conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627987/ https://www.ncbi.nlm.nih.gov/pubmed/31137498 http://dx.doi.org/10.3390/bios9020063 |
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