Cargando…

Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms

[Image: see text] Multidrug resistant bacterial infections threaten to become the number one cause of death by the year 2050. Development of antimicrobial dendritic polymers is considered promising as an alternative infection control strategy. For antimicrobial dendritic polymers to effectively kill...

Descripción completa

Detalles Bibliográficos
Autores principales: Rozenbaum, René T., Andrén, Oliver C. J., van der Mei, Henny C., Woudstra, Willem, Busscher, Henk J., Malkoch, Michael, Sharma, Prashant K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628176/
https://www.ncbi.nlm.nih.gov/pubmed/31142116
http://dx.doi.org/10.1021/acs.nanolett.9b00838
_version_ 1783434904308023296
author Rozenbaum, René T.
Andrén, Oliver C. J.
van der Mei, Henny C.
Woudstra, Willem
Busscher, Henk J.
Malkoch, Michael
Sharma, Prashant K.
author_facet Rozenbaum, René T.
Andrén, Oliver C. J.
van der Mei, Henny C.
Woudstra, Willem
Busscher, Henk J.
Malkoch, Michael
Sharma, Prashant K.
author_sort Rozenbaum, René T.
collection PubMed
description [Image: see text] Multidrug resistant bacterial infections threaten to become the number one cause of death by the year 2050. Development of antimicrobial dendritic polymers is considered promising as an alternative infection control strategy. For antimicrobial dendritic polymers to effectively kill bacteria residing in infectious biofilms, they have to penetrate and accumulate deep into biofilms. Biofilms are often recalcitrant to antimicrobial penetration and accumulation. Therefore, this work aims to determine the role of compact dendrons with different peripheral composition in their penetration into Pseudomonas aeruginosa biofilms. Red fluorescently labeled dendrons with pH-responsive NH(3)(+) peripheral groups initially penetrated faster from a buffer suspension at pH 7.0 into the acidic environment of P. aeruginosa biofilms than dendrons with OH or COO(–) groups at their periphery. In addition, dendrons with NH(3)(+) peripheral groups accumulated near the top of the biofilm due to electrostatic double-layer attraction with negatively charged biofilm components. However, accumulation of dendrons with OH and COO(–) peripheral groups was more evenly distributed across the depth of the biofilms than NH(3)(+) composed dendrons and exceeded accumulation of NH(3)(+) composed dendrons after 10 min of exposure. Unlike dendrons with NH(3)(+) groups at their periphery, dendrons with OH or COO(–) peripheral groups, lacking strong electrostatic double-layer attraction with biofilm components, were largely washed-out during exposure to PBS without dendrons. Thus, penetration and accumulation of dendrons into biofilms is controlled by their peripheral composition through electrostatic double-layer interactions, which is an important finding for the further development of new antimicrobial or antimicrobial-carrying dendritic polymers.
format Online
Article
Text
id pubmed-6628176
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-66281762019-07-16 Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms Rozenbaum, René T. Andrén, Oliver C. J. van der Mei, Henny C. Woudstra, Willem Busscher, Henk J. Malkoch, Michael Sharma, Prashant K. Nano Lett [Image: see text] Multidrug resistant bacterial infections threaten to become the number one cause of death by the year 2050. Development of antimicrobial dendritic polymers is considered promising as an alternative infection control strategy. For antimicrobial dendritic polymers to effectively kill bacteria residing in infectious biofilms, they have to penetrate and accumulate deep into biofilms. Biofilms are often recalcitrant to antimicrobial penetration and accumulation. Therefore, this work aims to determine the role of compact dendrons with different peripheral composition in their penetration into Pseudomonas aeruginosa biofilms. Red fluorescently labeled dendrons with pH-responsive NH(3)(+) peripheral groups initially penetrated faster from a buffer suspension at pH 7.0 into the acidic environment of P. aeruginosa biofilms than dendrons with OH or COO(–) groups at their periphery. In addition, dendrons with NH(3)(+) peripheral groups accumulated near the top of the biofilm due to electrostatic double-layer attraction with negatively charged biofilm components. However, accumulation of dendrons with OH and COO(–) peripheral groups was more evenly distributed across the depth of the biofilms than NH(3)(+) composed dendrons and exceeded accumulation of NH(3)(+) composed dendrons after 10 min of exposure. Unlike dendrons with NH(3)(+) groups at their periphery, dendrons with OH or COO(–) peripheral groups, lacking strong electrostatic double-layer attraction with biofilm components, were largely washed-out during exposure to PBS without dendrons. Thus, penetration and accumulation of dendrons into biofilms is controlled by their peripheral composition through electrostatic double-layer interactions, which is an important finding for the further development of new antimicrobial or antimicrobial-carrying dendritic polymers. American Chemical Society 2019-05-30 2019-07-10 /pmc/articles/PMC6628176/ /pubmed/31142116 http://dx.doi.org/10.1021/acs.nanolett.9b00838 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Rozenbaum, René T.
Andrén, Oliver C. J.
van der Mei, Henny C.
Woudstra, Willem
Busscher, Henk J.
Malkoch, Michael
Sharma, Prashant K.
Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title_full Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title_fullStr Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title_full_unstemmed Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title_short Penetration and Accumulation of Dendrons with Different Peripheral Composition in Pseudomonas aeruginosa Biofilms
title_sort penetration and accumulation of dendrons with different peripheral composition in pseudomonas aeruginosa biofilms
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628176/
https://www.ncbi.nlm.nih.gov/pubmed/31142116
http://dx.doi.org/10.1021/acs.nanolett.9b00838
work_keys_str_mv AT rozenbaumrenet penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT andrenolivercj penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT vandermeihennyc penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT woudstrawillem penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT busscherhenkj penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT malkochmichael penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms
AT sharmaprashantk penetrationandaccumulationofdendronswithdifferentperipheralcompositioninpseudomonasaeruginosabiofilms