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Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium

The integration of microfluidics with advanced biosensor technologies offers tremendous advantages such as smaller sample volume requirement and precise handling of samples and reagents, for developing affordable point-of-care testing methodologies that could be used in hospitals for monitoring pati...

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Autores principales: Surendran, Vikram, Chiulli, Thomas, Manoharan, Swetha, Knisley, Stephen, Packirisamy, Muthukumaran, Chandrasekaran, Arvind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628211/
https://www.ncbi.nlm.nih.gov/pubmed/31141923
http://dx.doi.org/10.3390/bios9020073
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author Surendran, Vikram
Chiulli, Thomas
Manoharan, Swetha
Knisley, Stephen
Packirisamy, Muthukumaran
Chandrasekaran, Arvind
author_facet Surendran, Vikram
Chiulli, Thomas
Manoharan, Swetha
Knisley, Stephen
Packirisamy, Muthukumaran
Chandrasekaran, Arvind
author_sort Surendran, Vikram
collection PubMed
description The integration of microfluidics with advanced biosensor technologies offers tremendous advantages such as smaller sample volume requirement and precise handling of samples and reagents, for developing affordable point-of-care testing methodologies that could be used in hospitals for monitoring patients. However, the success and popularity of point-of-care diagnosis lies with the generation of instantaneous and reliable results through in situ tests conducted in a painless, non-invasive manner. This work presents the development of a simple, hybrid integrated optical microfluidic biosensor for rapid detection of analytes in test samples. The proposed biosensor works on the principle of colorimetric optical absorption, wherein samples mixed with suitable chromogenic substrates induce a color change dependent upon the analyte concentration that could then be detected by the absorbance of light in its path length. This optical detection scheme has been hybrid integrated with an acoustofluidic micromixing unit to enable uniform mixing of fluids within the device. As a proof-of-concept, we have demonstrated the real-time application of our biosensor format for the detection of potassium in whole saliva samples. The results show that our lab-on-a-chip technology could provide a useful strategy in biomedical diagnoses for rapid analyte detection towards clinical point-of-care testing applications.
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spelling pubmed-66282112019-07-23 Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium Surendran, Vikram Chiulli, Thomas Manoharan, Swetha Knisley, Stephen Packirisamy, Muthukumaran Chandrasekaran, Arvind Biosensors (Basel) Article The integration of microfluidics with advanced biosensor technologies offers tremendous advantages such as smaller sample volume requirement and precise handling of samples and reagents, for developing affordable point-of-care testing methodologies that could be used in hospitals for monitoring patients. However, the success and popularity of point-of-care diagnosis lies with the generation of instantaneous and reliable results through in situ tests conducted in a painless, non-invasive manner. This work presents the development of a simple, hybrid integrated optical microfluidic biosensor for rapid detection of analytes in test samples. The proposed biosensor works on the principle of colorimetric optical absorption, wherein samples mixed with suitable chromogenic substrates induce a color change dependent upon the analyte concentration that could then be detected by the absorbance of light in its path length. This optical detection scheme has been hybrid integrated with an acoustofluidic micromixing unit to enable uniform mixing of fluids within the device. As a proof-of-concept, we have demonstrated the real-time application of our biosensor format for the detection of potassium in whole saliva samples. The results show that our lab-on-a-chip technology could provide a useful strategy in biomedical diagnoses for rapid analyte detection towards clinical point-of-care testing applications. MDPI 2019-05-28 /pmc/articles/PMC6628211/ /pubmed/31141923 http://dx.doi.org/10.3390/bios9020073 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Surendran, Vikram
Chiulli, Thomas
Manoharan, Swetha
Knisley, Stephen
Packirisamy, Muthukumaran
Chandrasekaran, Arvind
Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title_full Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title_fullStr Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title_full_unstemmed Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title_short Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium
title_sort acoustofluidic micromixing enabled hybrid integrated colorimetric sensing, for rapid point-of-care measurement of salivary potassium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628211/
https://www.ncbi.nlm.nih.gov/pubmed/31141923
http://dx.doi.org/10.3390/bios9020073
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