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Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile

Variability in plasma carotenoids may be attributable to several factors including genetic variants and lipid profile. Until now, the impact of DNA methylation on this variability has not been widely studied. Weighted gene correlation network analysis (WGCNA) is a systems biology method used for fin...

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Autores principales: Tremblay, Bénédicte L., Guénard, Frédéric, Lamarche, Benoît, Pérusse, Louis, Vohl, Marie-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628241/
https://www.ncbi.nlm.nih.gov/pubmed/31167428
http://dx.doi.org/10.3390/nu11061265
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author Tremblay, Bénédicte L.
Guénard, Frédéric
Lamarche, Benoît
Pérusse, Louis
Vohl, Marie-Claude
author_facet Tremblay, Bénédicte L.
Guénard, Frédéric
Lamarche, Benoît
Pérusse, Louis
Vohl, Marie-Claude
author_sort Tremblay, Bénédicte L.
collection PubMed
description Variability in plasma carotenoids may be attributable to several factors including genetic variants and lipid profile. Until now, the impact of DNA methylation on this variability has not been widely studied. Weighted gene correlation network analysis (WGCNA) is a systems biology method used for finding gene clusters (modules) with highly correlated methylation levels and for relating them to phenotypic traits. The objective of the present study was to examine the role of DNA methylation in the relationship between plasma total carotenoid concentrations and lipid profile using WGCNA in 48 healthy subjects. Genome-wide DNA methylation levels of 20,687 out of 472,245 CpG sites in blood leukocytes were associated with total carotenoid concentrations. Using WGCNA, nine co-methylation modules were identified. A total of 2734 hub genes (17 unique top hub genes) were potentially related to lipid profile. This study provides evidence for the potential implications of gene co-methylation in the relationship between plasma carotenoids and lipid profile. Further studies and validation of the hub genes are needed.
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spelling pubmed-66282412019-07-23 Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile Tremblay, Bénédicte L. Guénard, Frédéric Lamarche, Benoît Pérusse, Louis Vohl, Marie-Claude Nutrients Article Variability in plasma carotenoids may be attributable to several factors including genetic variants and lipid profile. Until now, the impact of DNA methylation on this variability has not been widely studied. Weighted gene correlation network analysis (WGCNA) is a systems biology method used for finding gene clusters (modules) with highly correlated methylation levels and for relating them to phenotypic traits. The objective of the present study was to examine the role of DNA methylation in the relationship between plasma total carotenoid concentrations and lipid profile using WGCNA in 48 healthy subjects. Genome-wide DNA methylation levels of 20,687 out of 472,245 CpG sites in blood leukocytes were associated with total carotenoid concentrations. Using WGCNA, nine co-methylation modules were identified. A total of 2734 hub genes (17 unique top hub genes) were potentially related to lipid profile. This study provides evidence for the potential implications of gene co-methylation in the relationship between plasma carotenoids and lipid profile. Further studies and validation of the hub genes are needed. MDPI 2019-06-04 /pmc/articles/PMC6628241/ /pubmed/31167428 http://dx.doi.org/10.3390/nu11061265 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tremblay, Bénédicte L.
Guénard, Frédéric
Lamarche, Benoît
Pérusse, Louis
Vohl, Marie-Claude
Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title_full Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title_fullStr Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title_full_unstemmed Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title_short Network Analysis of the Potential Role of DNA Methylation in the Relationship between Plasma Carotenoids and Lipid Profile
title_sort network analysis of the potential role of dna methylation in the relationship between plasma carotenoids and lipid profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628241/
https://www.ncbi.nlm.nih.gov/pubmed/31167428
http://dx.doi.org/10.3390/nu11061265
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