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An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model

Head and neck squamous cell carcinoma is a highly malignant disease and research is needed to find new therapeutic approaches. Faithful experimental models are required for this purpose. Here, we describe the specific cell culture conditions enabling the efficient establishment of primary cell cultu...

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Autores principales: Oppel, Felix, Shao, Senyao, Schürmann, Matthias, Goon, Peter, Albers, Andreas E., Sudhoff, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628367/
https://www.ncbi.nlm.nih.gov/pubmed/31181618
http://dx.doi.org/10.3390/cells8060555
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author Oppel, Felix
Shao, Senyao
Schürmann, Matthias
Goon, Peter
Albers, Andreas E.
Sudhoff, Holger
author_facet Oppel, Felix
Shao, Senyao
Schürmann, Matthias
Goon, Peter
Albers, Andreas E.
Sudhoff, Holger
author_sort Oppel, Felix
collection PubMed
description Head and neck squamous cell carcinoma is a highly malignant disease and research is needed to find new therapeutic approaches. Faithful experimental models are required for this purpose. Here, we describe the specific cell culture conditions enabling the efficient establishment of primary cell culture models. Whereas a classical 10% serum-containing medium resulted in the growth of fibroblast-like cells that outcompeted epithelial cells, we found that the use of specific culture conditions enabled the growth of epithelial tumor cells from HPV+ and HPV− head and neck cancer tissue applicable for research. EpCAM and high Thy-1 positivity on the cell surface were mutually exclusive and distinguished epithelial and fibroblast-like subpopulations in all primary cultures examined and thus can be used to monitor stromal contamination and epithelial cell content. Interestingly, cells of an individual patient developed tumor spheroids in suspension without the use of ultra-low attachment plates, whereas all other samples exclusively formed adherent cell layers. Spheroid cells were highly positive for ALDH1A1 and hence displayed a phenotype reminiscent of tumor stem cells. Altogether, we present a system to establish valuable primary cell culture models from head and neck cancer tissue at high efficiency that might be applicable in other tumor entities as well.
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spelling pubmed-66283672019-07-23 An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model Oppel, Felix Shao, Senyao Schürmann, Matthias Goon, Peter Albers, Andreas E. Sudhoff, Holger Cells Article Head and neck squamous cell carcinoma is a highly malignant disease and research is needed to find new therapeutic approaches. Faithful experimental models are required for this purpose. Here, we describe the specific cell culture conditions enabling the efficient establishment of primary cell culture models. Whereas a classical 10% serum-containing medium resulted in the growth of fibroblast-like cells that outcompeted epithelial cells, we found that the use of specific culture conditions enabled the growth of epithelial tumor cells from HPV+ and HPV− head and neck cancer tissue applicable for research. EpCAM and high Thy-1 positivity on the cell surface were mutually exclusive and distinguished epithelial and fibroblast-like subpopulations in all primary cultures examined and thus can be used to monitor stromal contamination and epithelial cell content. Interestingly, cells of an individual patient developed tumor spheroids in suspension without the use of ultra-low attachment plates, whereas all other samples exclusively formed adherent cell layers. Spheroid cells were highly positive for ALDH1A1 and hence displayed a phenotype reminiscent of tumor stem cells. Altogether, we present a system to establish valuable primary cell culture models from head and neck cancer tissue at high efficiency that might be applicable in other tumor entities as well. MDPI 2019-06-07 /pmc/articles/PMC6628367/ /pubmed/31181618 http://dx.doi.org/10.3390/cells8060555 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oppel, Felix
Shao, Senyao
Schürmann, Matthias
Goon, Peter
Albers, Andreas E.
Sudhoff, Holger
An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title_full An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title_fullStr An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title_full_unstemmed An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title_short An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model
title_sort effective primary head and neck squamous cell carcinoma in vitro model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628367/
https://www.ncbi.nlm.nih.gov/pubmed/31181618
http://dx.doi.org/10.3390/cells8060555
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