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Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages

Non-thermal atmospheric pressure plasma sources operated in ambient environments are known to generate a variety of reactive oxygen and nitrogen species which could be applied for various biomedical applications. Herein, we fabricate a micro-dielectric barrier discharge plasma device by using screen...

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Autores principales: Kaushik, Nagendra Kumar, Kaushik, Neha, Adhikari, Manish, Ghimire, Bhagirath, Linh, Nguyen Nhat, Mishra, Yogendra Kumar, Lee, Su-Jae, Choi, Eun Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628390/
https://www.ncbi.nlm.nih.gov/pubmed/31216715
http://dx.doi.org/10.3390/cancers11060842
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author Kaushik, Nagendra Kumar
Kaushik, Neha
Adhikari, Manish
Ghimire, Bhagirath
Linh, Nguyen Nhat
Mishra, Yogendra Kumar
Lee, Su-Jae
Choi, Eun Ha
author_facet Kaushik, Nagendra Kumar
Kaushik, Neha
Adhikari, Manish
Ghimire, Bhagirath
Linh, Nguyen Nhat
Mishra, Yogendra Kumar
Lee, Su-Jae
Choi, Eun Ha
author_sort Kaushik, Nagendra Kumar
collection PubMed
description Non-thermal atmospheric pressure plasma sources operated in ambient environments are known to generate a variety of reactive oxygen and nitrogen species which could be applied for various biomedical applications. Herein, we fabricate a micro-dielectric barrier discharge plasma device by using screen-printing technology and apply it for studying immuno-stimulatory effects. We demonstrate a tumor-suppressive role for plasma-stimulated macrophages in metastatic solid cancers that directly elicit proliferation and are responsible for tumor relapse mediated by mesenchymal shift. Using microarray analysis, we observed that cold plasma stimulates and differentiates monocyte cells into macrophages as demonstrated by expression of several cytokine/chemokine markers. Moreover, plasma treatment stimulates the differentiation of pro-inflammatory (M1) macrophages to a greater extent. These stimulated macrophages favor anti-tumorigenic immune responses against metastasis acquisition and cancer stem cell maintenance in solid cancers in vitro. Differentiation of monocytes into anticancer macrophages could improve the efficacy of plasma treatment, especially in modifying pro-tumor inflammatory microenvironment through effecting highly resistant immunosuppressive tumor cells associated with tumor relapse.
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spelling pubmed-66283902019-07-23 Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages Kaushik, Nagendra Kumar Kaushik, Neha Adhikari, Manish Ghimire, Bhagirath Linh, Nguyen Nhat Mishra, Yogendra Kumar Lee, Su-Jae Choi, Eun Ha Cancers (Basel) Article Non-thermal atmospheric pressure plasma sources operated in ambient environments are known to generate a variety of reactive oxygen and nitrogen species which could be applied for various biomedical applications. Herein, we fabricate a micro-dielectric barrier discharge plasma device by using screen-printing technology and apply it for studying immuno-stimulatory effects. We demonstrate a tumor-suppressive role for plasma-stimulated macrophages in metastatic solid cancers that directly elicit proliferation and are responsible for tumor relapse mediated by mesenchymal shift. Using microarray analysis, we observed that cold plasma stimulates and differentiates monocyte cells into macrophages as demonstrated by expression of several cytokine/chemokine markers. Moreover, plasma treatment stimulates the differentiation of pro-inflammatory (M1) macrophages to a greater extent. These stimulated macrophages favor anti-tumorigenic immune responses against metastasis acquisition and cancer stem cell maintenance in solid cancers in vitro. Differentiation of monocytes into anticancer macrophages could improve the efficacy of plasma treatment, especially in modifying pro-tumor inflammatory microenvironment through effecting highly resistant immunosuppressive tumor cells associated with tumor relapse. MDPI 2019-06-18 /pmc/articles/PMC6628390/ /pubmed/31216715 http://dx.doi.org/10.3390/cancers11060842 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaushik, Nagendra Kumar
Kaushik, Neha
Adhikari, Manish
Ghimire, Bhagirath
Linh, Nguyen Nhat
Mishra, Yogendra Kumar
Lee, Su-Jae
Choi, Eun Ha
Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title_full Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title_fullStr Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title_full_unstemmed Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title_short Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages
title_sort preventing the solid cancer progression via release of anticancer-cytokines in co-culture with cold plasma-stimulated macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628390/
https://www.ncbi.nlm.nih.gov/pubmed/31216715
http://dx.doi.org/10.3390/cancers11060842
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