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Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee

Ochratoxin A (OTA) produced by mycotoxigenic fungi (Aspergillus and Penicillium spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted...

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Autores principales: Casas-Junco, Paloma Patricia, Solís-Pacheco, Josué Raymundo, Ragazzo-Sánchez, Juan Arturo, Aguilar-Uscanga, Blanca Rosa, Bautista-Rosales, Pedro Ulises, Calderón-Santoyo, Montserrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628446/
https://www.ncbi.nlm.nih.gov/pubmed/31200476
http://dx.doi.org/10.3390/toxins11060337
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author Casas-Junco, Paloma Patricia
Solís-Pacheco, Josué Raymundo
Ragazzo-Sánchez, Juan Arturo
Aguilar-Uscanga, Blanca Rosa
Bautista-Rosales, Pedro Ulises
Calderón-Santoyo, Montserrat
author_facet Casas-Junco, Paloma Patricia
Solís-Pacheco, Josué Raymundo
Ragazzo-Sánchez, Juan Arturo
Aguilar-Uscanga, Blanca Rosa
Bautista-Rosales, Pedro Ulises
Calderón-Santoyo, Montserrat
author_sort Casas-Junco, Paloma Patricia
collection PubMed
description Ochratoxin A (OTA) produced by mycotoxigenic fungi (Aspergillus and Penicillium spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted coffee samples were artificially inoculated with A. westerdijikiae, A. steynii, A. versicolor, and A. niger, and incubated at 27 °C over 21 days for OTA production. Samples were cold plasma treated at 30 W input power and 850 V output voltage with helium at 1.5 L/min flow. OTA production in coffee was analyzed by high performance liquid chromatography coupled to a mass spectrometer (HPLC-MS). After 6 min of treatment with cold plasma, fungi were completely inhibited (4 log reduction). Cold plasma reduces 50% of OTA content after 30 min of treatment. Toxicity was estimated for extracts of artificially contaminated roasted coffee samples using the brine shrimp (Artemia salina) lethality assay. Toxicity for untreated roasted coffee was shown to be “toxic”, while toxicity for cold plasma treated coffee was reduced to “slightly toxic”. These results suggested that cold plasma may be considered as an alternative method for the degradation and reduction of toxin production by mycotoxigenic fungi in the processing of foods and feedstuffs.
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spelling pubmed-66284462019-07-23 Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee Casas-Junco, Paloma Patricia Solís-Pacheco, Josué Raymundo Ragazzo-Sánchez, Juan Arturo Aguilar-Uscanga, Blanca Rosa Bautista-Rosales, Pedro Ulises Calderón-Santoyo, Montserrat Toxins (Basel) Article Ochratoxin A (OTA) produced by mycotoxigenic fungi (Aspergillus and Penicillium spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted coffee samples were artificially inoculated with A. westerdijikiae, A. steynii, A. versicolor, and A. niger, and incubated at 27 °C over 21 days for OTA production. Samples were cold plasma treated at 30 W input power and 850 V output voltage with helium at 1.5 L/min flow. OTA production in coffee was analyzed by high performance liquid chromatography coupled to a mass spectrometer (HPLC-MS). After 6 min of treatment with cold plasma, fungi were completely inhibited (4 log reduction). Cold plasma reduces 50% of OTA content after 30 min of treatment. Toxicity was estimated for extracts of artificially contaminated roasted coffee samples using the brine shrimp (Artemia salina) lethality assay. Toxicity for untreated roasted coffee was shown to be “toxic”, while toxicity for cold plasma treated coffee was reduced to “slightly toxic”. These results suggested that cold plasma may be considered as an alternative method for the degradation and reduction of toxin production by mycotoxigenic fungi in the processing of foods and feedstuffs. MDPI 2019-06-13 /pmc/articles/PMC6628446/ /pubmed/31200476 http://dx.doi.org/10.3390/toxins11060337 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Casas-Junco, Paloma Patricia
Solís-Pacheco, Josué Raymundo
Ragazzo-Sánchez, Juan Arturo
Aguilar-Uscanga, Blanca Rosa
Bautista-Rosales, Pedro Ulises
Calderón-Santoyo, Montserrat
Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title_full Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title_fullStr Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title_full_unstemmed Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title_short Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
title_sort cold plasma treatment as an alternative for ochratoxin a detoxification and inhibition of mycotoxigenic fungi in roasted coffee
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628446/
https://www.ncbi.nlm.nih.gov/pubmed/31200476
http://dx.doi.org/10.3390/toxins11060337
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