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Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation

Zearalenone (ZEN) is an estrogenic mycotoxin which can cause loss in animal production. The aim of this study was to screen Bacillus strains for their ZEN detoxification capability and use a fermentation process to validate their potential application in the feed industry. In the high-level ZEN-cont...

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Autores principales: Chen, Shiau-Wei, Wang, Han-Tsung, Shih, Wei-Yuan, Ciou, Yan-An, Chang, Yu-Yi, Ananda, Laurensia, Wang, Shu-Yin, Hsu, Jih-Tay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628455/
https://www.ncbi.nlm.nih.gov/pubmed/31181798
http://dx.doi.org/10.3390/toxins11060330
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author Chen, Shiau-Wei
Wang, Han-Tsung
Shih, Wei-Yuan
Ciou, Yan-An
Chang, Yu-Yi
Ananda, Laurensia
Wang, Shu-Yin
Hsu, Jih-Tay
author_facet Chen, Shiau-Wei
Wang, Han-Tsung
Shih, Wei-Yuan
Ciou, Yan-An
Chang, Yu-Yi
Ananda, Laurensia
Wang, Shu-Yin
Hsu, Jih-Tay
author_sort Chen, Shiau-Wei
collection PubMed
description Zearalenone (ZEN) is an estrogenic mycotoxin which can cause loss in animal production. The aim of this study was to screen Bacillus strains for their ZEN detoxification capability and use a fermentation process to validate their potential application in the feed industry. In the high-level ZEN-contaminated maize (5 mg·kg(−1)) fermentation test, B2 strain exhibited the highest detoxification rate, removing 56% of the ZEN. However, B2 strain was not the strain with the highest ZEN detoxification in the culturing media. When B2 grew in TSB medium with ZEN, it had higher bacterial numbers, lactic acid, acetic acid, total volatile fatty acids, and ammonia nitrogen. The ZEN-contaminated maize fermented by B2 strain had better fermentation characteristics (lactic acid > 110 mmol·L(−1); acetic acid < 20 mmol·L(−1); pH < 4.5) than ZEN-free maize. Furthermore, B2 also had detoxification capabilities toward aflatoxins B1, deoxynivalenol, fumonisin B1, and T2 toxin. Our study demonstrated differences in screening outcome between bacterial culturing conditions and the maize fermentation process. This is important for the feed industry to consider when choosing a proper method to screen candidate isolates for the pretreatment of ZEN-contaminated maize. It appears that using the fermentation process to address the ZEN-contaminated maize problem in animal feed is a reliable choice.
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spelling pubmed-66284552019-07-23 Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation Chen, Shiau-Wei Wang, Han-Tsung Shih, Wei-Yuan Ciou, Yan-An Chang, Yu-Yi Ananda, Laurensia Wang, Shu-Yin Hsu, Jih-Tay Toxins (Basel) Article Zearalenone (ZEN) is an estrogenic mycotoxin which can cause loss in animal production. The aim of this study was to screen Bacillus strains for their ZEN detoxification capability and use a fermentation process to validate their potential application in the feed industry. In the high-level ZEN-contaminated maize (5 mg·kg(−1)) fermentation test, B2 strain exhibited the highest detoxification rate, removing 56% of the ZEN. However, B2 strain was not the strain with the highest ZEN detoxification in the culturing media. When B2 grew in TSB medium with ZEN, it had higher bacterial numbers, lactic acid, acetic acid, total volatile fatty acids, and ammonia nitrogen. The ZEN-contaminated maize fermented by B2 strain had better fermentation characteristics (lactic acid > 110 mmol·L(−1); acetic acid < 20 mmol·L(−1); pH < 4.5) than ZEN-free maize. Furthermore, B2 also had detoxification capabilities toward aflatoxins B1, deoxynivalenol, fumonisin B1, and T2 toxin. Our study demonstrated differences in screening outcome between bacterial culturing conditions and the maize fermentation process. This is important for the feed industry to consider when choosing a proper method to screen candidate isolates for the pretreatment of ZEN-contaminated maize. It appears that using the fermentation process to address the ZEN-contaminated maize problem in animal feed is a reliable choice. MDPI 2019-06-08 /pmc/articles/PMC6628455/ /pubmed/31181798 http://dx.doi.org/10.3390/toxins11060330 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Shiau-Wei
Wang, Han-Tsung
Shih, Wei-Yuan
Ciou, Yan-An
Chang, Yu-Yi
Ananda, Laurensia
Wang, Shu-Yin
Hsu, Jih-Tay
Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title_full Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title_fullStr Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title_full_unstemmed Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title_short Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
title_sort application of zearalenone (zen)-detoxifying bacillus in animal feed decontamination through fermentation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628455/
https://www.ncbi.nlm.nih.gov/pubmed/31181798
http://dx.doi.org/10.3390/toxins11060330
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