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Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe

The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is an invasive species in North America and Europe that damages many different host plants. Substantial work has been conducted on the genetic diversity and invasion pathways of H. halys in some of the countries where it ha...

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Autores principales: Kapantaidaki, Despoina Ev., Evangelou, Vassiliki I., Morrison, William R., Leskey, Tracy C., Brodeur, Jacques, Milonas, Panagiotis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628459/
https://www.ncbi.nlm.nih.gov/pubmed/31212913
http://dx.doi.org/10.3390/insects10060174
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author Kapantaidaki, Despoina Ev.
Evangelou, Vassiliki I.
Morrison, William R.
Leskey, Tracy C.
Brodeur, Jacques
Milonas, Panagiotis
author_facet Kapantaidaki, Despoina Ev.
Evangelou, Vassiliki I.
Morrison, William R.
Leskey, Tracy C.
Brodeur, Jacques
Milonas, Panagiotis
author_sort Kapantaidaki, Despoina Ev.
collection PubMed
description The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is an invasive species in North America and Europe that damages many different host plants. Substantial work has been conducted on the genetic diversity and invasion pathways of H. halys in some of the countries where it has been found, based on mitochondrial sequences. The main objective of the present study was to further explore the genetic diversity of invasive populations of H. halys exploiting both mitochondrial and nuclear markers. We used two molecular markers: the mitochondrial Cytochrome Oxidase I (COI) gene, an ideal standardized molecular marker for distinguishing closely related species, and the ribosomal Internal Transcribed Spacer 1 (ITS1), because only a few sequences of H. halys exist to this point in global databases. We used specimens from eight populations from Greece, Italy, Canada, and the US. Among the 14 haplotypes retrieved based on the mtCOI gene, two of them (H162–H163) were detected for the first time. These two haplotypes were found in specimens from Canada, Italy, and the US. Concerning the ITS1 region, 24 haplotypes were identified, with 15 being unique for a sampled population. In Greece and the US, 14 and 12 haplotypes were found, respectively, with 7 and 6 of them being unique for Greece and the US, respectively. Our analysis of the nuclear genes of H. halys indicates high genetic diversity of the invading populations in North America and Europe.
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spelling pubmed-66284592019-07-23 Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe Kapantaidaki, Despoina Ev. Evangelou, Vassiliki I. Morrison, William R. Leskey, Tracy C. Brodeur, Jacques Milonas, Panagiotis Insects Article The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is an invasive species in North America and Europe that damages many different host plants. Substantial work has been conducted on the genetic diversity and invasion pathways of H. halys in some of the countries where it has been found, based on mitochondrial sequences. The main objective of the present study was to further explore the genetic diversity of invasive populations of H. halys exploiting both mitochondrial and nuclear markers. We used two molecular markers: the mitochondrial Cytochrome Oxidase I (COI) gene, an ideal standardized molecular marker for distinguishing closely related species, and the ribosomal Internal Transcribed Spacer 1 (ITS1), because only a few sequences of H. halys exist to this point in global databases. We used specimens from eight populations from Greece, Italy, Canada, and the US. Among the 14 haplotypes retrieved based on the mtCOI gene, two of them (H162–H163) were detected for the first time. These two haplotypes were found in specimens from Canada, Italy, and the US. Concerning the ITS1 region, 24 haplotypes were identified, with 15 being unique for a sampled population. In Greece and the US, 14 and 12 haplotypes were found, respectively, with 7 and 6 of them being unique for Greece and the US, respectively. Our analysis of the nuclear genes of H. halys indicates high genetic diversity of the invading populations in North America and Europe. MDPI 2019-06-17 /pmc/articles/PMC6628459/ /pubmed/31212913 http://dx.doi.org/10.3390/insects10060174 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kapantaidaki, Despoina Ev.
Evangelou, Vassiliki I.
Morrison, William R.
Leskey, Tracy C.
Brodeur, Jacques
Milonas, Panagiotis
Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title_full Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title_fullStr Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title_full_unstemmed Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title_short Halyomorpha halys (Hemiptera: Pentatomidae) Genetic Diversity in North America and Europe
title_sort halyomorpha halys (hemiptera: pentatomidae) genetic diversity in north america and europe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628459/
https://www.ncbi.nlm.nih.gov/pubmed/31212913
http://dx.doi.org/10.3390/insects10060174
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