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Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents
Leptin is an adipokine that regulates adipose tissue mass through membrane-anchored leptin receptor (Ob-R). Extracellular domain of Ob-R in plasma is called soluble leptin receptor (sOb-R), and is the main leptin-binding protein. Based on a previous DNA microarray analysis that showed induction of h...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629078/ https://www.ncbi.nlm.nih.gov/pubmed/31306448 http://dx.doi.org/10.1371/journal.pone.0219603 |
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author | Yamada, Riho Odamaki, Shizuka Araki, Masaya Watanabe, Tasuku Matsuo, Keigo Uchida, Kaito Kato, Taku Ozaki-Masuzawa, Yori Takenaka, Asako |
author_facet | Yamada, Riho Odamaki, Shizuka Araki, Masaya Watanabe, Tasuku Matsuo, Keigo Uchida, Kaito Kato, Taku Ozaki-Masuzawa, Yori Takenaka, Asako |
author_sort | Yamada, Riho |
collection | PubMed |
description | Leptin is an adipokine that regulates adipose tissue mass through membrane-anchored leptin receptor (Ob-R). Extracellular domain of Ob-R in plasma is called soluble leptin receptor (sOb-R), and is the main leptin-binding protein. Based on a previous DNA microarray analysis that showed induction of hepatic Ob-R mRNA in low-protein diet-fed mice, this study aimed to clarify the effect of dietary protein restriction on hepatic Ob-R mRNA and plasma sOb-R levels. First, the effect of protein restriction on hepatic Ob-R mRNA level was examined together with fasting and food restriction using male rats as common experimental model for nutritional research. Hepatic Ob-R mRNA level was increased by feeding low-protein diet for 7 d, although not significantly influenced by 12-h fasting and sixty percent restriction in food consumption. Then, effect of protein restriction on liver Ob-R and plasma sOb-R was investigated using male mice because specific sOb-R ELISA was more available for mice. Hepatic Ob-R mRNA level was also increased in protein restricted-mice although it did not increase in hypothalamus. Hepatic Ob-R protein was decreased, whereas plasma sOb-R was increased by protein restriction. Because the concentration of sOb-R increased without changing plasma leptin concentration, free leptin in plasma was significantly reduced. The direct effect of amino acid deprivation on Ob-R mRNA level was not observed in rat hepatoma cells H4IIE cultured in amino acid deprived medium. In conclusion, dietary protein restriction increased hepatic Ob-R mRNA, resulting in increased plasma sOb-R concentration, which in turn, reduces plasma free leptin level and may modulate leptin activity. |
format | Online Article Text |
id | pubmed-6629078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66290782019-07-25 Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents Yamada, Riho Odamaki, Shizuka Araki, Masaya Watanabe, Tasuku Matsuo, Keigo Uchida, Kaito Kato, Taku Ozaki-Masuzawa, Yori Takenaka, Asako PLoS One Research Article Leptin is an adipokine that regulates adipose tissue mass through membrane-anchored leptin receptor (Ob-R). Extracellular domain of Ob-R in plasma is called soluble leptin receptor (sOb-R), and is the main leptin-binding protein. Based on a previous DNA microarray analysis that showed induction of hepatic Ob-R mRNA in low-protein diet-fed mice, this study aimed to clarify the effect of dietary protein restriction on hepatic Ob-R mRNA and plasma sOb-R levels. First, the effect of protein restriction on hepatic Ob-R mRNA level was examined together with fasting and food restriction using male rats as common experimental model for nutritional research. Hepatic Ob-R mRNA level was increased by feeding low-protein diet for 7 d, although not significantly influenced by 12-h fasting and sixty percent restriction in food consumption. Then, effect of protein restriction on liver Ob-R and plasma sOb-R was investigated using male mice because specific sOb-R ELISA was more available for mice. Hepatic Ob-R mRNA level was also increased in protein restricted-mice although it did not increase in hypothalamus. Hepatic Ob-R protein was decreased, whereas plasma sOb-R was increased by protein restriction. Because the concentration of sOb-R increased without changing plasma leptin concentration, free leptin in plasma was significantly reduced. The direct effect of amino acid deprivation on Ob-R mRNA level was not observed in rat hepatoma cells H4IIE cultured in amino acid deprived medium. In conclusion, dietary protein restriction increased hepatic Ob-R mRNA, resulting in increased plasma sOb-R concentration, which in turn, reduces plasma free leptin level and may modulate leptin activity. Public Library of Science 2019-07-15 /pmc/articles/PMC6629078/ /pubmed/31306448 http://dx.doi.org/10.1371/journal.pone.0219603 Text en © 2019 Yamada et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yamada, Riho Odamaki, Shizuka Araki, Masaya Watanabe, Tasuku Matsuo, Keigo Uchida, Kaito Kato, Taku Ozaki-Masuzawa, Yori Takenaka, Asako Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title | Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title_full | Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title_fullStr | Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title_full_unstemmed | Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title_short | Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents |
title_sort | dietary protein restriction increases hepatic leptin receptor mrna and plasma soluble leptin receptor in male rodents |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629078/ https://www.ncbi.nlm.nih.gov/pubmed/31306448 http://dx.doi.org/10.1371/journal.pone.0219603 |
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