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Necroptosis was found in a rat ischemia/reperfusion injury flap model

BACKGROUND: Necroptosis is a new form of cell death that has been identified as a third pathway causing cell death. In this study, necrostatin-1 (Nec-1) was used to determine whether necroptosis exists in a rat ischaemia/reperfusion injury flap model. METHODS: In this study, twenty male Sprague-Dawl...

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Autores principales: Liu, Hao, Zhang, Ming-Zi, Liu, Yi-Fang, Dong, Xin-Hang, Hao, Yan, Wang, You-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629296/
https://www.ncbi.nlm.nih.gov/pubmed/30628958
http://dx.doi.org/10.1097/CM9.0000000000000005
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author Liu, Hao
Zhang, Ming-Zi
Liu, Yi-Fang
Dong, Xin-Hang
Hao, Yan
Wang, You-Bin
author_facet Liu, Hao
Zhang, Ming-Zi
Liu, Yi-Fang
Dong, Xin-Hang
Hao, Yan
Wang, You-Bin
author_sort Liu, Hao
collection PubMed
description BACKGROUND: Necroptosis is a new form of cell death that has been identified as a third pathway causing cell death. In this study, necrostatin-1 (Nec-1) was used to determine whether necroptosis exists in a rat ischaemia/reperfusion injury flap model. METHODS: In this study, twenty male Sprague-Dawley rats were divided randomly into two groups: a control group (CTL group) and a Nec-1 group. Each abdominal skin flap underwent 3 h of ischaemia and then reperfusion. Fifteen minutes before and after reperfusion, phosphate buffer saline (PBS) was administered intraperitoneally to the CTL group, while Nec-1 was administered intraperitoneally to the Nec-1 group. Twenty-four hours after reperfusion, the whole flap was divided equally into 54 sections. Flap blood perfusion was measured. One sample was taken randomly from each row. Morphological changes, apoptosis, receptor-interacting protein-1 (RIP-1) expression and caspase-3 activity were observed and detected. The measurements between the two groups were compared with the independent t-test, and a P value of <0.05 was considered statistically significant. RESULTS: Compared to flaps in the CTL group, flaps in the Nec-1 group showed longer survival rates, better blood perfusion and less inflammatory infiltration. The total flap area considered to have survived was 70.88 ± 10.28% in the CTL group, whereas 80.56 ± 5.40% of the area was found to be living in the Nec-1 group (Nec-1 vs. CTL, t = –2.624, P < 0.05). For some rows, there were significant differences in cell apoptosis between the two groups, the apoptosis index (AI) in rows “9 cm”, “7 cm”, “6 cm” and “5 cm” was significantly lower in the Nec-1 group than that in the CTL group (Nec-1 vs. CTL, P < 0.05). RIP-1 expression was much lower in the Nec-1 group than that in the CTL group in rows “5 cm” to “9 cm” (Nec-1 vs. CTL, P < 0.05). No significant differences in caspase-3 activity were found. CONCLUSION: According to the results, necroptosis was present in a rat abdominal ischaemia/reperfusion injury flap model.
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spelling pubmed-66292962019-07-22 Necroptosis was found in a rat ischemia/reperfusion injury flap model Liu, Hao Zhang, Ming-Zi Liu, Yi-Fang Dong, Xin-Hang Hao, Yan Wang, You-Bin Chin Med J (Engl) Original Articles BACKGROUND: Necroptosis is a new form of cell death that has been identified as a third pathway causing cell death. In this study, necrostatin-1 (Nec-1) was used to determine whether necroptosis exists in a rat ischaemia/reperfusion injury flap model. METHODS: In this study, twenty male Sprague-Dawley rats were divided randomly into two groups: a control group (CTL group) and a Nec-1 group. Each abdominal skin flap underwent 3 h of ischaemia and then reperfusion. Fifteen minutes before and after reperfusion, phosphate buffer saline (PBS) was administered intraperitoneally to the CTL group, while Nec-1 was administered intraperitoneally to the Nec-1 group. Twenty-four hours after reperfusion, the whole flap was divided equally into 54 sections. Flap blood perfusion was measured. One sample was taken randomly from each row. Morphological changes, apoptosis, receptor-interacting protein-1 (RIP-1) expression and caspase-3 activity were observed and detected. The measurements between the two groups were compared with the independent t-test, and a P value of <0.05 was considered statistically significant. RESULTS: Compared to flaps in the CTL group, flaps in the Nec-1 group showed longer survival rates, better blood perfusion and less inflammatory infiltration. The total flap area considered to have survived was 70.88 ± 10.28% in the CTL group, whereas 80.56 ± 5.40% of the area was found to be living in the Nec-1 group (Nec-1 vs. CTL, t = –2.624, P < 0.05). For some rows, there were significant differences in cell apoptosis between the two groups, the apoptosis index (AI) in rows “9 cm”, “7 cm”, “6 cm” and “5 cm” was significantly lower in the Nec-1 group than that in the CTL group (Nec-1 vs. CTL, P < 0.05). RIP-1 expression was much lower in the Nec-1 group than that in the CTL group in rows “5 cm” to “9 cm” (Nec-1 vs. CTL, P < 0.05). No significant differences in caspase-3 activity were found. CONCLUSION: According to the results, necroptosis was present in a rat abdominal ischaemia/reperfusion injury flap model. Wolters Kluwer Health 2019-01-05 2019-01-05 /pmc/articles/PMC6629296/ /pubmed/30628958 http://dx.doi.org/10.1097/CM9.0000000000000005 Text en Copyright © 2018 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0
spellingShingle Original Articles
Liu, Hao
Zhang, Ming-Zi
Liu, Yi-Fang
Dong, Xin-Hang
Hao, Yan
Wang, You-Bin
Necroptosis was found in a rat ischemia/reperfusion injury flap model
title Necroptosis was found in a rat ischemia/reperfusion injury flap model
title_full Necroptosis was found in a rat ischemia/reperfusion injury flap model
title_fullStr Necroptosis was found in a rat ischemia/reperfusion injury flap model
title_full_unstemmed Necroptosis was found in a rat ischemia/reperfusion injury flap model
title_short Necroptosis was found in a rat ischemia/reperfusion injury flap model
title_sort necroptosis was found in a rat ischemia/reperfusion injury flap model
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629296/
https://www.ncbi.nlm.nih.gov/pubmed/30628958
http://dx.doi.org/10.1097/CM9.0000000000000005
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