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Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus

Itaconic acid is used as a bio-based, renewable building block in the polymer industry. It is produced by submerged fermentations of the filamentous fungus Aspergillus terreus from molasses or starch, but research over the efficient utilization of non-food, lignocellulosic plant biomass is soaring....

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Autores principales: Kolláth, István S., Molnár, Ákos P., Soós, Áron, Fekete, Erzsébet, Sándor, Erzsébet, Kovács, Béla, Kubicek, Christian P., Karaffa, Levente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629873/
https://www.ncbi.nlm.nih.gov/pubmed/31338087
http://dx.doi.org/10.3389/fmicb.2019.01589
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author Kolláth, István S.
Molnár, Ákos P.
Soós, Áron
Fekete, Erzsébet
Sándor, Erzsébet
Kovács, Béla
Kubicek, Christian P.
Karaffa, Levente
author_facet Kolláth, István S.
Molnár, Ákos P.
Soós, Áron
Fekete, Erzsébet
Sándor, Erzsébet
Kovács, Béla
Kubicek, Christian P.
Karaffa, Levente
author_sort Kolláth, István S.
collection PubMed
description Itaconic acid is used as a bio-based, renewable building block in the polymer industry. It is produced by submerged fermentations of the filamentous fungus Aspergillus terreus from molasses or starch, but research over the efficient utilization of non-food, lignocellulosic plant biomass is soaring. The objective of this study was to test whether the application of two key cultivation parameters for obtaining itaconic acid from D-glucose in high yields – Mn(2+) ion deficiency and high concentration of the carbon source – would also occur on D-xylose, the principal monomer of lignocellulose. To this end, a carbon and energy balance for itaconic acid formation was established, which is 0.83 moles/mole D-xylose. The effect of Mn(2+) ions on itaconic acid formation was indeed similar to that on D-glucose and maximal yields were obtained below 3 μg L(–1) Mn(2+) ions, which were, however, only 0.63 moles of itaconic acid per mole D-xylose. In contrast to the case on D-glucose, increasing D-xylose concentration over 50 g L(–1) did not change the above yield. By-products such as xylitol and α-ketoglutarate were found, but in total they remained below 2% of the concentration of D-xylose. Mass balance of the fermentation with 110 g L(–1) D-xylose revealed that >95% of the carbon from D-xylose was accounted as biomass, itaconic acid, and the carbon dioxide released in the last step of itaconic acid biosynthesis. Our data show that the efficiency of biomass formation is the critical parameter for itaconic acid yield from D-xylose under otherwise optimal conditions.
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spelling pubmed-66298732019-07-23 Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus Kolláth, István S. Molnár, Ákos P. Soós, Áron Fekete, Erzsébet Sándor, Erzsébet Kovács, Béla Kubicek, Christian P. Karaffa, Levente Front Microbiol Microbiology Itaconic acid is used as a bio-based, renewable building block in the polymer industry. It is produced by submerged fermentations of the filamentous fungus Aspergillus terreus from molasses or starch, but research over the efficient utilization of non-food, lignocellulosic plant biomass is soaring. The objective of this study was to test whether the application of two key cultivation parameters for obtaining itaconic acid from D-glucose in high yields – Mn(2+) ion deficiency and high concentration of the carbon source – would also occur on D-xylose, the principal monomer of lignocellulose. To this end, a carbon and energy balance for itaconic acid formation was established, which is 0.83 moles/mole D-xylose. The effect of Mn(2+) ions on itaconic acid formation was indeed similar to that on D-glucose and maximal yields were obtained below 3 μg L(–1) Mn(2+) ions, which were, however, only 0.63 moles of itaconic acid per mole D-xylose. In contrast to the case on D-glucose, increasing D-xylose concentration over 50 g L(–1) did not change the above yield. By-products such as xylitol and α-ketoglutarate were found, but in total they remained below 2% of the concentration of D-xylose. Mass balance of the fermentation with 110 g L(–1) D-xylose revealed that >95% of the carbon from D-xylose was accounted as biomass, itaconic acid, and the carbon dioxide released in the last step of itaconic acid biosynthesis. Our data show that the efficiency of biomass formation is the critical parameter for itaconic acid yield from D-xylose under otherwise optimal conditions. Frontiers Media S.A. 2019-07-09 /pmc/articles/PMC6629873/ /pubmed/31338087 http://dx.doi.org/10.3389/fmicb.2019.01589 Text en Copyright © 2019 Kolláth, Molnár, Soós, Fekete, Sándor, Kovács, Kubicek and Karaffa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kolláth, István S.
Molnár, Ákos P.
Soós, Áron
Fekete, Erzsébet
Sándor, Erzsébet
Kovács, Béla
Kubicek, Christian P.
Karaffa, Levente
Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title_full Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title_fullStr Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title_full_unstemmed Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title_short Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus
title_sort manganese deficiency is required for high itaconic acid production from d-xylose in aspergillus terreus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629873/
https://www.ncbi.nlm.nih.gov/pubmed/31338087
http://dx.doi.org/10.3389/fmicb.2019.01589
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