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Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling

Freezing damages in forages represents a major economic loss to agriculture. This study was conducted to investigate the effects of freeze–thaw (FT) event on microbial community dynamics of red clover silage. Results showed that the FT-treated material displayed higher proportions of Weissella and a...

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Autores principales: Dong, Zhihao, Li, Junfeng, Chen, Lei, Wang, Siran, Shao, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629895/
https://www.ncbi.nlm.nih.gov/pubmed/31338085
http://dx.doi.org/10.3389/fmicb.2019.01559
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author Dong, Zhihao
Li, Junfeng
Chen, Lei
Wang, Siran
Shao, Tao
author_facet Dong, Zhihao
Li, Junfeng
Chen, Lei
Wang, Siran
Shao, Tao
author_sort Dong, Zhihao
collection PubMed
description Freezing damages in forages represents a major economic loss to agriculture. This study was conducted to investigate the effects of freeze–thaw (FT) event on microbial community dynamics of red clover silage. Results showed that the FT-treated material displayed higher proportions of Weissella and aerobic bacteria, while lower Pantoea and Enterobacter compared with the control material. The FT event promoted the development of Lactobacillus in silage microflora, inducing more intense lactic fermentation after an initial short lag. The aerobic bacteria were suppressed immediately after the onset of ensiling. Microbiomes of the two silages tended to be almost similar after 2 days of ensiling. However, a small number of aerobic bacteria tended to revitalize in the FT silage with prolonged ensiling time, indicated by apparent abundances of Acinetobacter and Pseudomonas at the end of ensiling. The results obtained here suggest that the FT event could promote the development of Lactobacillus during ensiling and the control of aerobe revitalization need to be concerned with silages made from the freeze-damaged forages.
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spelling pubmed-66298952019-07-23 Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling Dong, Zhihao Li, Junfeng Chen, Lei Wang, Siran Shao, Tao Front Microbiol Microbiology Freezing damages in forages represents a major economic loss to agriculture. This study was conducted to investigate the effects of freeze–thaw (FT) event on microbial community dynamics of red clover silage. Results showed that the FT-treated material displayed higher proportions of Weissella and aerobic bacteria, while lower Pantoea and Enterobacter compared with the control material. The FT event promoted the development of Lactobacillus in silage microflora, inducing more intense lactic fermentation after an initial short lag. The aerobic bacteria were suppressed immediately after the onset of ensiling. Microbiomes of the two silages tended to be almost similar after 2 days of ensiling. However, a small number of aerobic bacteria tended to revitalize in the FT silage with prolonged ensiling time, indicated by apparent abundances of Acinetobacter and Pseudomonas at the end of ensiling. The results obtained here suggest that the FT event could promote the development of Lactobacillus during ensiling and the control of aerobe revitalization need to be concerned with silages made from the freeze-damaged forages. Frontiers Media S.A. 2019-07-09 /pmc/articles/PMC6629895/ /pubmed/31338085 http://dx.doi.org/10.3389/fmicb.2019.01559 Text en Copyright © 2019 Dong, Li, Chen, Wang and Shao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dong, Zhihao
Li, Junfeng
Chen, Lei
Wang, Siran
Shao, Tao
Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title_full Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title_fullStr Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title_full_unstemmed Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title_short Effects of Freeze–Thaw Event on Microbial Community Dynamics During Red Clover Ensiling
title_sort effects of freeze–thaw event on microbial community dynamics during red clover ensiling
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629895/
https://www.ncbi.nlm.nih.gov/pubmed/31338085
http://dx.doi.org/10.3389/fmicb.2019.01559
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