Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...

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Autores principales: Marsian, Johanna, Hurdiss, Daniel L., Ranson, Neil A., Ritala, Anneli, Paley, Richard, Cano, Irene, Lomonossoff, George P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/
https://www.ncbi.nlm.nih.gov/pubmed/31354759
http://dx.doi.org/10.3389/fpls.2019.00880
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author Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
author_facet Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
author_sort Marsian, Johanna
collection PubMed
description Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
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spelling pubmed-66299392019-07-26 Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. Front Plant Sci Plant Science Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. Frontiers Media S.A. 2019-07-09 /pmc/articles/PMC6629939/ /pubmed/31354759 http://dx.doi.org/10.3389/fpls.2019.00880 Text en Copyright © 2019 Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossoff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_fullStr Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full_unstemmed Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_short Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_sort plant-made nervous necrosis virus-like particles protect fish against disease
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/
https://www.ncbi.nlm.nih.gov/pubmed/31354759
http://dx.doi.org/10.3389/fpls.2019.00880
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