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Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC

Ruthenium organometallic compounds represent an attractive avenue in developing alternatives to platinum-based chemotherapeutic agents. While evidence has been presented indicating ruthenium-based compounds interact with isolated DNA in vitro, it is unclear what effect these compounds exert in cells...

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Autores principales: Fast, Olivia G., Gentry, Brittany, Strouth, Liah, Niece, Madison B., Beckford, Floyd A., Shell, Steven M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629949/
https://www.ncbi.nlm.nih.gov/pubmed/31227614
http://dx.doi.org/10.1042/BSR20190378
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author Fast, Olivia G.
Gentry, Brittany
Strouth, Liah
Niece, Madison B.
Beckford, Floyd A.
Shell, Steven M.
author_facet Fast, Olivia G.
Gentry, Brittany
Strouth, Liah
Niece, Madison B.
Beckford, Floyd A.
Shell, Steven M.
author_sort Fast, Olivia G.
collection PubMed
description Ruthenium organometallic compounds represent an attractive avenue in developing alternatives to platinum-based chemotherapeutic agents. While evidence has been presented indicating ruthenium-based compounds interact with isolated DNA in vitro, it is unclear what effect these compounds exert in cells. Moreover, the antibiotic efficacy of polynuclear ruthenium organometallic compounds remains uncertain. In the present study, we report that exposure to polynuclear ruthenium organometallic compounds induces recruitment of damaged DNA sensing protein Xeroderma pigmentosum Group C into chromatin-immobilized foci. Additionally, we observed one of the tested polynuclear ruthenium organometallic compounds displayed increased cytotoxicity against human cells deficient in nucleotide excision repair (NER). Taken together, these results suggest that polynuclear ruthenium organometallic compounds induce DNA damage in cells, and that cellular resistance to these compounds may be influenced by the NER DNA repair phenotype of the cells.
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spelling pubmed-66299492019-07-24 Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC Fast, Olivia G. Gentry, Brittany Strouth, Liah Niece, Madison B. Beckford, Floyd A. Shell, Steven M. Biosci Rep Research Articles Ruthenium organometallic compounds represent an attractive avenue in developing alternatives to platinum-based chemotherapeutic agents. While evidence has been presented indicating ruthenium-based compounds interact with isolated DNA in vitro, it is unclear what effect these compounds exert in cells. Moreover, the antibiotic efficacy of polynuclear ruthenium organometallic compounds remains uncertain. In the present study, we report that exposure to polynuclear ruthenium organometallic compounds induces recruitment of damaged DNA sensing protein Xeroderma pigmentosum Group C into chromatin-immobilized foci. Additionally, we observed one of the tested polynuclear ruthenium organometallic compounds displayed increased cytotoxicity against human cells deficient in nucleotide excision repair (NER). Taken together, these results suggest that polynuclear ruthenium organometallic compounds induce DNA damage in cells, and that cellular resistance to these compounds may be influenced by the NER DNA repair phenotype of the cells. Portland Press Ltd. 2019-07-16 /pmc/articles/PMC6629949/ /pubmed/31227614 http://dx.doi.org/10.1042/BSR20190378 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Fast, Olivia G.
Gentry, Brittany
Strouth, Liah
Niece, Madison B.
Beckford, Floyd A.
Shell, Steven M.
Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title_full Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title_fullStr Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title_full_unstemmed Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title_short Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC
title_sort polynuclear ruthenium organometallic compounds induce dna damage in human cells identified by the nucleotide excision repair factor xpc
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629949/
https://www.ncbi.nlm.nih.gov/pubmed/31227614
http://dx.doi.org/10.1042/BSR20190378
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