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Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli
Numerous studies have focused on the bacterial synthesis of palladium nanoparticles (bio-Pd NPs), via uptake of Pd (II) ions and their enzymatically-mediated reduction to Pd (0). Cells of Desulfovibrio desulfuricans (obligate anaerobe) and Escherichia coli (facultative anaerobe, grown anaerobically)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630224/ https://www.ncbi.nlm.nih.gov/pubmed/31195655 http://dx.doi.org/10.3390/nano9060857 |
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author | Gomez-Bolivar, Jaime Mikheenko, Iryna P. Macaskie, Lynne E. Merroun, Mohamed L. |
author_facet | Gomez-Bolivar, Jaime Mikheenko, Iryna P. Macaskie, Lynne E. Merroun, Mohamed L. |
author_sort | Gomez-Bolivar, Jaime |
collection | PubMed |
description | Numerous studies have focused on the bacterial synthesis of palladium nanoparticles (bio-Pd NPs), via uptake of Pd (II) ions and their enzymatically-mediated reduction to Pd (0). Cells of Desulfovibrio desulfuricans (obligate anaerobe) and Escherichia coli (facultative anaerobe, grown anaerobically) were exposed to low-dose radiofrequency (RF) radiation(microwave (MW) energy) and the biosynthesized Pd NPs were compared. Resting cells were exposed to microwave energy before Pd (II)-challenge. MW-injured Pd (II)-treated cells (and non MW-treated controls) were contacted with H(2) to promote Pd(II) reduction. By using scanning transmission electron microscopy (STEM) associated with a high-angle annular dark field (HAADF) detector and energy dispersive X-ray (EDX) spectrometry, the respective Pd NPs were compared with respect to their mean sizes, size distribution, location, composition, and structure. Differences were observed following MWinjury prior to Pd(II) exposure versus uninjured controls. With D. desulfuricans the bio-Pd NPs formed post-injury showed two NP populations with different sizes and morphologies. The first, mainly periplasmically-located, showed polycrystalline Pd nano-branches with different crystal orientations and sizes ranging between 20 and 30 nm. The second NPpopulation, mainly located intracellularly, comprised single crystals with sizes between 1 and 5 nm. Bio-Pd NPs were produced mainly intracellularly by injured cells of E. coli and comprised single crystals with a size distribution between 1 and 3 nm. The polydispersity index was reduced in the bio-Pd made by injured cells of E. coli and D. desulfuricans to 32% and 39%, respectively, of the values of uninjured controls, indicating an increase in NP homogeneity of 30–40% as a result of the prior MWinjury. The observations are discussed with respect to the different locations of Pd(II)-reducing hydrogenases in the two organisms and with respect to potential implications for the catalytic activity of the produced NPs following injury-associated altered NP patterning. |
format | Online Article Text |
id | pubmed-6630224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66302242019-08-19 Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli Gomez-Bolivar, Jaime Mikheenko, Iryna P. Macaskie, Lynne E. Merroun, Mohamed L. Nanomaterials (Basel) Article Numerous studies have focused on the bacterial synthesis of palladium nanoparticles (bio-Pd NPs), via uptake of Pd (II) ions and their enzymatically-mediated reduction to Pd (0). Cells of Desulfovibrio desulfuricans (obligate anaerobe) and Escherichia coli (facultative anaerobe, grown anaerobically) were exposed to low-dose radiofrequency (RF) radiation(microwave (MW) energy) and the biosynthesized Pd NPs were compared. Resting cells were exposed to microwave energy before Pd (II)-challenge. MW-injured Pd (II)-treated cells (and non MW-treated controls) were contacted with H(2) to promote Pd(II) reduction. By using scanning transmission electron microscopy (STEM) associated with a high-angle annular dark field (HAADF) detector and energy dispersive X-ray (EDX) spectrometry, the respective Pd NPs were compared with respect to their mean sizes, size distribution, location, composition, and structure. Differences were observed following MWinjury prior to Pd(II) exposure versus uninjured controls. With D. desulfuricans the bio-Pd NPs formed post-injury showed two NP populations with different sizes and morphologies. The first, mainly periplasmically-located, showed polycrystalline Pd nano-branches with different crystal orientations and sizes ranging between 20 and 30 nm. The second NPpopulation, mainly located intracellularly, comprised single crystals with sizes between 1 and 5 nm. Bio-Pd NPs were produced mainly intracellularly by injured cells of E. coli and comprised single crystals with a size distribution between 1 and 3 nm. The polydispersity index was reduced in the bio-Pd made by injured cells of E. coli and D. desulfuricans to 32% and 39%, respectively, of the values of uninjured controls, indicating an increase in NP homogeneity of 30–40% as a result of the prior MWinjury. The observations are discussed with respect to the different locations of Pd(II)-reducing hydrogenases in the two organisms and with respect to potential implications for the catalytic activity of the produced NPs following injury-associated altered NP patterning. MDPI 2019-06-05 /pmc/articles/PMC6630224/ /pubmed/31195655 http://dx.doi.org/10.3390/nano9060857 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gomez-Bolivar, Jaime Mikheenko, Iryna P. Macaskie, Lynne E. Merroun, Mohamed L. Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title | Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title_full | Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title_fullStr | Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title_full_unstemmed | Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title_short | Characterization of Palladium Nanoparticles Produced by Healthy and Microwave-Injured Cells of Desulfovibrio desulfuricans and Escherichia coli |
title_sort | characterization of palladium nanoparticles produced by healthy and microwave-injured cells of desulfovibrio desulfuricans and escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630224/ https://www.ncbi.nlm.nih.gov/pubmed/31195655 http://dx.doi.org/10.3390/nano9060857 |
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