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Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk

Helicobacter pylori (H. pylori) represents an independent risk factor for Gastric Cancer (GC). First Degree Relatives (FDR) of GC subjects and Autoimmune Gastritis (AG) patients are both at increased risk for GC. H. pylori genetic heterogeneity within the gastric niche of FDR and AG individuals has...

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Autores principales: Casarotto, Mariateresa, Pratesi, Chiara, Bidoli, Ettore, Maiero, Stefania, Magris, Raffaella, Steffan, Agostino, Basaglia, Giancarlo, Canzonieri, Vincenzo, De Re, Valli, Cannizzaro, Renato, Zanussi, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630233/
https://www.ncbi.nlm.nih.gov/pubmed/31109082
http://dx.doi.org/10.3390/pathogens8020065
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author Casarotto, Mariateresa
Pratesi, Chiara
Bidoli, Ettore
Maiero, Stefania
Magris, Raffaella
Steffan, Agostino
Basaglia, Giancarlo
Canzonieri, Vincenzo
De Re, Valli
Cannizzaro, Renato
Zanussi, Stefania
author_facet Casarotto, Mariateresa
Pratesi, Chiara
Bidoli, Ettore
Maiero, Stefania
Magris, Raffaella
Steffan, Agostino
Basaglia, Giancarlo
Canzonieri, Vincenzo
De Re, Valli
Cannizzaro, Renato
Zanussi, Stefania
author_sort Casarotto, Mariateresa
collection PubMed
description Helicobacter pylori (H. pylori) represents an independent risk factor for Gastric Cancer (GC). First Degree Relatives (FDR) of GC subjects and Autoimmune Gastritis (AG) patients are both at increased risk for GC. H. pylori genetic heterogeneity within the gastric niche of FDR and AG individuals has been little explored. To understand whether they exploit an increased H. pylori stability and virulence, 14 AG, 25 FDR, 39 GC and 13 dyspeptic patients (D) were investigated by a cultural PCR-based approach characterizing single colonies-forming-units. We chose three loci within the Cytotoxin-associated gene-A Pathogenicity Island (CagPAI) (cagA,cagE,virB11), vacA, homA and homB as markers of virulence with reported association to GC. Inflammatory/precancerous lesions were staged according to Sydney System. When compared to D, FDR, similarly to GC patients, were associated to higher atrophy (OR = 6.29; 95% CI:1.23–31.96 in FDR; OR = 7.50; 95% CI:1.67–33.72 in GC) and a lower frequency of mixed infections (OR = 0.16; 95% CI:0.03–0.81 in FDR; OR = 0.10; 95% CI:0.02–0.48 in GC). FDR presented also an increased neutrophil infiltration (OR = 7.19; 95% CI:1.16–44.65). Both FDR and GC carried a higher proportion of CagPAI(+)vacAs1i1mx(+)homB(+) profiles (OR = 2.71; 95% CI: 1.66–4.41 and OR = 3.43; 95% CI: 2.16–5.44, respectively). Conversely, AG patients presented a lower frequency of subtypes carrying a stable CagPAI and vacAs1i1mx. These results underline different H. pylori plasticity in FDR and AG individuals, and thus, a different host-bacterium interaction capacity that should be considered in the context of eradication therapies.
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spelling pubmed-66302332019-08-19 Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk Casarotto, Mariateresa Pratesi, Chiara Bidoli, Ettore Maiero, Stefania Magris, Raffaella Steffan, Agostino Basaglia, Giancarlo Canzonieri, Vincenzo De Re, Valli Cannizzaro, Renato Zanussi, Stefania Pathogens Article Helicobacter pylori (H. pylori) represents an independent risk factor for Gastric Cancer (GC). First Degree Relatives (FDR) of GC subjects and Autoimmune Gastritis (AG) patients are both at increased risk for GC. H. pylori genetic heterogeneity within the gastric niche of FDR and AG individuals has been little explored. To understand whether they exploit an increased H. pylori stability and virulence, 14 AG, 25 FDR, 39 GC and 13 dyspeptic patients (D) were investigated by a cultural PCR-based approach characterizing single colonies-forming-units. We chose three loci within the Cytotoxin-associated gene-A Pathogenicity Island (CagPAI) (cagA,cagE,virB11), vacA, homA and homB as markers of virulence with reported association to GC. Inflammatory/precancerous lesions were staged according to Sydney System. When compared to D, FDR, similarly to GC patients, were associated to higher atrophy (OR = 6.29; 95% CI:1.23–31.96 in FDR; OR = 7.50; 95% CI:1.67–33.72 in GC) and a lower frequency of mixed infections (OR = 0.16; 95% CI:0.03–0.81 in FDR; OR = 0.10; 95% CI:0.02–0.48 in GC). FDR presented also an increased neutrophil infiltration (OR = 7.19; 95% CI:1.16–44.65). Both FDR and GC carried a higher proportion of CagPAI(+)vacAs1i1mx(+)homB(+) profiles (OR = 2.71; 95% CI: 1.66–4.41 and OR = 3.43; 95% CI: 2.16–5.44, respectively). Conversely, AG patients presented a lower frequency of subtypes carrying a stable CagPAI and vacAs1i1mx. These results underline different H. pylori plasticity in FDR and AG individuals, and thus, a different host-bacterium interaction capacity that should be considered in the context of eradication therapies. MDPI 2019-05-18 /pmc/articles/PMC6630233/ /pubmed/31109082 http://dx.doi.org/10.3390/pathogens8020065 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Casarotto, Mariateresa
Pratesi, Chiara
Bidoli, Ettore
Maiero, Stefania
Magris, Raffaella
Steffan, Agostino
Basaglia, Giancarlo
Canzonieri, Vincenzo
De Re, Valli
Cannizzaro, Renato
Zanussi, Stefania
Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title_full Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title_fullStr Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title_full_unstemmed Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title_short Differential Helicobacter pylori Plasticity in the Gastric Niche of Subjects at Increased Gastric Cancer Risk
title_sort differential helicobacter pylori plasticity in the gastric niche of subjects at increased gastric cancer risk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630233/
https://www.ncbi.nlm.nih.gov/pubmed/31109082
http://dx.doi.org/10.3390/pathogens8020065
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