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Bridged Nucleic Acids Reloaded
Oligonucleotides are key compounds widely used for research, diagnostics, and therapeutics. The rapid increase in oligonucleotide-based applications, together with the progress in nucleic acids research, has led to the design of nucleotide analogs that, when part of these oligomers, enhance their ef...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630285/ https://www.ncbi.nlm.nih.gov/pubmed/31234313 http://dx.doi.org/10.3390/molecules24122297 |
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author | Soler-Bistué, Alfonso Zorreguieta, Angeles Tolmasky, Marcelo E. |
author_facet | Soler-Bistué, Alfonso Zorreguieta, Angeles Tolmasky, Marcelo E. |
author_sort | Soler-Bistué, Alfonso |
collection | PubMed |
description | Oligonucleotides are key compounds widely used for research, diagnostics, and therapeutics. The rapid increase in oligonucleotide-based applications, together with the progress in nucleic acids research, has led to the design of nucleotide analogs that, when part of these oligomers, enhance their efficiency, bioavailability, or stability. One of the most useful nucleotide analogs is the first-generation bridged nucleic acids (BNA), also known as locked nucleic acids (LNA), which were used in combination with ribonucleotides, deoxyribonucleotides, or other analogs to construct oligomers with diverse applications. However, there is still room to improve their efficiency, bioavailability, stability, and, importantly, toxicity. A second-generation BNA, BNA(NC) (2′-O,4′-aminoethylene bridged nucleic acid), has been recently made available. Oligomers containing these analogs not only showed less toxicity when compared to LNA-containing compounds but, in some cases, also exhibited higher specificity. Although there are still few applications where BNA(NC)-containing compounds have been researched, the promising results warrant more effort in incorporating these analogs for other applications. Furthermore, newer BNA compounds will be introduced in the near future, offering great hope to oligonucleotide-based fields of research and applications. |
format | Online Article Text |
id | pubmed-6630285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66302852019-08-19 Bridged Nucleic Acids Reloaded Soler-Bistué, Alfonso Zorreguieta, Angeles Tolmasky, Marcelo E. Molecules Review Oligonucleotides are key compounds widely used for research, diagnostics, and therapeutics. The rapid increase in oligonucleotide-based applications, together with the progress in nucleic acids research, has led to the design of nucleotide analogs that, when part of these oligomers, enhance their efficiency, bioavailability, or stability. One of the most useful nucleotide analogs is the first-generation bridged nucleic acids (BNA), also known as locked nucleic acids (LNA), which were used in combination with ribonucleotides, deoxyribonucleotides, or other analogs to construct oligomers with diverse applications. However, there is still room to improve their efficiency, bioavailability, stability, and, importantly, toxicity. A second-generation BNA, BNA(NC) (2′-O,4′-aminoethylene bridged nucleic acid), has been recently made available. Oligomers containing these analogs not only showed less toxicity when compared to LNA-containing compounds but, in some cases, also exhibited higher specificity. Although there are still few applications where BNA(NC)-containing compounds have been researched, the promising results warrant more effort in incorporating these analogs for other applications. Furthermore, newer BNA compounds will be introduced in the near future, offering great hope to oligonucleotide-based fields of research and applications. MDPI 2019-06-21 /pmc/articles/PMC6630285/ /pubmed/31234313 http://dx.doi.org/10.3390/molecules24122297 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Soler-Bistué, Alfonso Zorreguieta, Angeles Tolmasky, Marcelo E. Bridged Nucleic Acids Reloaded |
title | Bridged Nucleic Acids Reloaded |
title_full | Bridged Nucleic Acids Reloaded |
title_fullStr | Bridged Nucleic Acids Reloaded |
title_full_unstemmed | Bridged Nucleic Acids Reloaded |
title_short | Bridged Nucleic Acids Reloaded |
title_sort | bridged nucleic acids reloaded |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630285/ https://www.ncbi.nlm.nih.gov/pubmed/31234313 http://dx.doi.org/10.3390/molecules24122297 |
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