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Cobas 4800 HPV detection in cervical samples of Polish women

INTRODUCTION: Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor – cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for th...

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Autores principales: Smolarz, Beata, Samulak, Dariusz, Szyłło, Krzysztof, Makowska, Marianna, Romanowicz, Hanna, Kojs, Zbigniew, Michalska, Magdalena M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630389/
https://www.ncbi.nlm.nih.gov/pubmed/31316291
http://dx.doi.org/10.5114/wo.2019.85880
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author Smolarz, Beata
Samulak, Dariusz
Szyłło, Krzysztof
Makowska, Marianna
Romanowicz, Hanna
Kojs, Zbigniew
Michalska, Magdalena M.
author_facet Smolarz, Beata
Samulak, Dariusz
Szyłło, Krzysztof
Makowska, Marianna
Romanowicz, Hanna
Kojs, Zbigniew
Michalska, Magdalena M.
author_sort Smolarz, Beata
collection PubMed
description INTRODUCTION: Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor – cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for the presence of HPV has increased the effectiveness of screening programs for the detection of this cancer. This study aimed to analyze the prevalence of high risk HPV DNA (HR HPV) in females from Poland. MATERIAL AND METHODS: The study was performed on 280 cervical smear samples. In this work we used the Roche Cobas 4800 HPV test to detect the HR HPV in cervical smear samples. RESULTS: 56 patients (20%) proved to be positive regarding HPV-16 DNA and 40 patients (14.28%) regarding HPV-18 DNA. In overall assessment, in 94 patients (33.57%) we detected oncogenic HPV subtypes, other than the two mentioned above. In 90 patients (32.14%) no high risk HPV was detected. CONCLUSIONS: The Roche Cobas 4800 HPV test is a viable, effective, easy and quick tool in detecting high risk HPV DNA.
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spelling pubmed-66303892019-07-17 Cobas 4800 HPV detection in cervical samples of Polish women Smolarz, Beata Samulak, Dariusz Szyłło, Krzysztof Makowska, Marianna Romanowicz, Hanna Kojs, Zbigniew Michalska, Magdalena M. Contemp Oncol (Pozn) Original Paper INTRODUCTION: Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor – cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for the presence of HPV has increased the effectiveness of screening programs for the detection of this cancer. This study aimed to analyze the prevalence of high risk HPV DNA (HR HPV) in females from Poland. MATERIAL AND METHODS: The study was performed on 280 cervical smear samples. In this work we used the Roche Cobas 4800 HPV test to detect the HR HPV in cervical smear samples. RESULTS: 56 patients (20%) proved to be positive regarding HPV-16 DNA and 40 patients (14.28%) regarding HPV-18 DNA. In overall assessment, in 94 patients (33.57%) we detected oncogenic HPV subtypes, other than the two mentioned above. In 90 patients (32.14%) no high risk HPV was detected. CONCLUSIONS: The Roche Cobas 4800 HPV test is a viable, effective, easy and quick tool in detecting high risk HPV DNA. Termedia Publishing House 2019-06-13 2019 /pmc/articles/PMC6630389/ /pubmed/31316291 http://dx.doi.org/10.5114/wo.2019.85880 Text en Copyright: © 2019 Termedia Sp. z o. o. http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Smolarz, Beata
Samulak, Dariusz
Szyłło, Krzysztof
Makowska, Marianna
Romanowicz, Hanna
Kojs, Zbigniew
Michalska, Magdalena M.
Cobas 4800 HPV detection in cervical samples of Polish women
title Cobas 4800 HPV detection in cervical samples of Polish women
title_full Cobas 4800 HPV detection in cervical samples of Polish women
title_fullStr Cobas 4800 HPV detection in cervical samples of Polish women
title_full_unstemmed Cobas 4800 HPV detection in cervical samples of Polish women
title_short Cobas 4800 HPV detection in cervical samples of Polish women
title_sort cobas 4800 hpv detection in cervical samples of polish women
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630389/
https://www.ncbi.nlm.nih.gov/pubmed/31316291
http://dx.doi.org/10.5114/wo.2019.85880
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