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A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing

In vitro permeation studies using nail clippings or nail plates are commonly used in the development of transungual formulations. However, there are ethical, safety and cost issues associated with sourcing such tissues. Herein, we describe a preliminary approach is described for the design and manuf...

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Autores principales: Sil, Bruno C., Patel, Avnish, Crowther, Jonathan M., Moore, David J., Hadgraft, Jonathan, Hilton, Stephen T., Lane, Majella E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630397/
https://www.ncbi.nlm.nih.gov/pubmed/31141993
http://dx.doi.org/10.3390/pharmaceutics11060250
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author Sil, Bruno C.
Patel, Avnish
Crowther, Jonathan M.
Moore, David J.
Hadgraft, Jonathan
Hilton, Stephen T.
Lane, Majella E.
author_facet Sil, Bruno C.
Patel, Avnish
Crowther, Jonathan M.
Moore, David J.
Hadgraft, Jonathan
Hilton, Stephen T.
Lane, Majella E.
author_sort Sil, Bruno C.
collection PubMed
description In vitro permeation studies using nail clippings or nail plates are commonly used in the development of transungual formulations. However, there are ethical, safety and cost issues associated with sourcing such tissues. Herein, we describe a preliminary approach is described for the design and manufacture of a human nail model surrogate based on 3D printing. To evaluate these 3D printed constructs, nails were mounted in conventional glass Franz cells and a commercial antifungal lacquer formulation containing ciclopirox olamine was applied daily to the surrogate printed surfaces for a period of 14 days. On days 8 and 14, the surfaces of the 3D printed nails were washed with ethanol to remove excess formulation. Confocal Raman spectroscopy (CRS) was used to profile the drug in the 3D printed nail. At the end of the Franz cell studies, no drug was observed in the receptor phase. CRS studies confirmed penetration of the active into the model nails with reproducible depth profiles. Our ongoing work is focused on synthesising commercial and non-commercial printable resins that can replicate the physical and chemical characteristics of the human nail. This will allow further evaluation of actives for ungual therapy and advance the development of the surrogate nail tissue model.
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spelling pubmed-66303972019-08-19 A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing Sil, Bruno C. Patel, Avnish Crowther, Jonathan M. Moore, David J. Hadgraft, Jonathan Hilton, Stephen T. Lane, Majella E. Pharmaceutics Communication In vitro permeation studies using nail clippings or nail plates are commonly used in the development of transungual formulations. However, there are ethical, safety and cost issues associated with sourcing such tissues. Herein, we describe a preliminary approach is described for the design and manufacture of a human nail model surrogate based on 3D printing. To evaluate these 3D printed constructs, nails were mounted in conventional glass Franz cells and a commercial antifungal lacquer formulation containing ciclopirox olamine was applied daily to the surrogate printed surfaces for a period of 14 days. On days 8 and 14, the surfaces of the 3D printed nails were washed with ethanol to remove excess formulation. Confocal Raman spectroscopy (CRS) was used to profile the drug in the 3D printed nail. At the end of the Franz cell studies, no drug was observed in the receptor phase. CRS studies confirmed penetration of the active into the model nails with reproducible depth profiles. Our ongoing work is focused on synthesising commercial and non-commercial printable resins that can replicate the physical and chemical characteristics of the human nail. This will allow further evaluation of actives for ungual therapy and advance the development of the surrogate nail tissue model. MDPI 2019-05-28 /pmc/articles/PMC6630397/ /pubmed/31141993 http://dx.doi.org/10.3390/pharmaceutics11060250 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Sil, Bruno C.
Patel, Avnish
Crowther, Jonathan M.
Moore, David J.
Hadgraft, Jonathan
Hilton, Stephen T.
Lane, Majella E.
A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title_full A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title_fullStr A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title_full_unstemmed A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title_short A Preliminary Investigation of Additive Manufacture to Fabricate Human Nail Plate Surrogates for Pharmaceutical Testing
title_sort preliminary investigation of additive manufacture to fabricate human nail plate surrogates for pharmaceutical testing
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630397/
https://www.ncbi.nlm.nih.gov/pubmed/31141993
http://dx.doi.org/10.3390/pharmaceutics11060250
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