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Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode
This paper describes a simple strategy for the ultratrace level detection of Pb(2+) ion based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO) electrode. First, ERGO was formed on a glassy carbon electrode (GCE) by the reduction of graphene oxide (GO) using cyclic voltammet...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630585/ https://www.ncbi.nlm.nih.gov/pubmed/31151250 http://dx.doi.org/10.3390/nano9060817 |
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author | Yu, Su Hwan Lee, Chang-Seuk Kim, Tae Hyun |
author_facet | Yu, Su Hwan Lee, Chang-Seuk Kim, Tae Hyun |
author_sort | Yu, Su Hwan |
collection | PubMed |
description | This paper describes a simple strategy for the ultratrace level detection of Pb(2+) ion based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO) electrode. First, ERGO was formed on a glassy carbon electrode (GCE) by the reduction of graphene oxide (GO) using cyclic voltammetry. Subsequently, a methylene blue (MB)-tagged, guanine-rich DNA aptamer (Apt) was attached to the surface of ERGO via π-π interaction, leading to the Apt-modified ERGO electrode. The presence of Pb(2+) could generate the folding of Apt to a G-quadruplex structure. The formation of G-quadruplex resulted in detaching the Apt from the ERGO/GCE, leading to a change in redox current of the MB tag. Electrochemical measurements showed the proposed sensor had an exceptional sensitivity for Pb(2+) with a linear range from 10(−15) to 10(−9) M and a detection limit of 0.51 fM. The sensor also exhibited high selectivity for Pb(2+), as well as many other advantages, such as stability, reproducibility, regeneration, as well as simple fabrication and operation processes. |
format | Online Article Text |
id | pubmed-6630585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66305852019-08-19 Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode Yu, Su Hwan Lee, Chang-Seuk Kim, Tae Hyun Nanomaterials (Basel) Article This paper describes a simple strategy for the ultratrace level detection of Pb(2+) ion based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO) electrode. First, ERGO was formed on a glassy carbon electrode (GCE) by the reduction of graphene oxide (GO) using cyclic voltammetry. Subsequently, a methylene blue (MB)-tagged, guanine-rich DNA aptamer (Apt) was attached to the surface of ERGO via π-π interaction, leading to the Apt-modified ERGO electrode. The presence of Pb(2+) could generate the folding of Apt to a G-quadruplex structure. The formation of G-quadruplex resulted in detaching the Apt from the ERGO/GCE, leading to a change in redox current of the MB tag. Electrochemical measurements showed the proposed sensor had an exceptional sensitivity for Pb(2+) with a linear range from 10(−15) to 10(−9) M and a detection limit of 0.51 fM. The sensor also exhibited high selectivity for Pb(2+), as well as many other advantages, such as stability, reproducibility, regeneration, as well as simple fabrication and operation processes. MDPI 2019-05-30 /pmc/articles/PMC6630585/ /pubmed/31151250 http://dx.doi.org/10.3390/nano9060817 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yu, Su Hwan Lee, Chang-Seuk Kim, Tae Hyun Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title | Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title_full | Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title_fullStr | Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title_full_unstemmed | Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title_short | Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode |
title_sort | electrochemical detection of ultratrace lead ion through attaching and detaching dna aptamer from electrochemically reduced graphene oxide electrode |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630585/ https://www.ncbi.nlm.nih.gov/pubmed/31151250 http://dx.doi.org/10.3390/nano9060817 |
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