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Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals
Formulation of multifunctional biopolymer-based scaffolds is one of the major focuses in modern tissue engineering and regenerative medicine. Besides proper mechanical/chemical properties, an ideal scaffold should: (i) possess a well-tuned porous internal structure for cell seeding/growth and (ii) h...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630714/ https://www.ncbi.nlm.nih.gov/pubmed/31146472 http://dx.doi.org/10.3390/mi10060357 |
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author | Sergeeva, Alena Vikulina, Anna S. Volodkin, Dmitry |
author_facet | Sergeeva, Alena Vikulina, Anna S. Volodkin, Dmitry |
author_sort | Sergeeva, Alena |
collection | PubMed |
description | Formulation of multifunctional biopolymer-based scaffolds is one of the major focuses in modern tissue engineering and regenerative medicine. Besides proper mechanical/chemical properties, an ideal scaffold should: (i) possess a well-tuned porous internal structure for cell seeding/growth and (ii) host bioactive molecules to be protected against biodegradation and presented to cells when required. Alginate hydrogels were extensively developed to serve as scaffolds, and recent advances in the hydrogel formulation demonstrate their applicability as “ideal” soft scaffolds. This review focuses on advanced porous alginate scaffolds (PAS) fabricated using hard templating on vaterite CaCO(3) crystals. These novel tailor-made soft structures can be prepared at physiologically relevant conditions offering a high level of control over their internal structure and high performance for loading/release of bioactive macromolecules. The novel approach to assemble PAS is compared with traditional methods used for fabrication of porous alginate hydrogels. Finally, future perspectives and applications of PAS for advanced cell culture, tissue engineering, and drug testing are discussed. |
format | Online Article Text |
id | pubmed-6630714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66307142019-08-19 Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals Sergeeva, Alena Vikulina, Anna S. Volodkin, Dmitry Micromachines (Basel) Review Formulation of multifunctional biopolymer-based scaffolds is one of the major focuses in modern tissue engineering and regenerative medicine. Besides proper mechanical/chemical properties, an ideal scaffold should: (i) possess a well-tuned porous internal structure for cell seeding/growth and (ii) host bioactive molecules to be protected against biodegradation and presented to cells when required. Alginate hydrogels were extensively developed to serve as scaffolds, and recent advances in the hydrogel formulation demonstrate their applicability as “ideal” soft scaffolds. This review focuses on advanced porous alginate scaffolds (PAS) fabricated using hard templating on vaterite CaCO(3) crystals. These novel tailor-made soft structures can be prepared at physiologically relevant conditions offering a high level of control over their internal structure and high performance for loading/release of bioactive macromolecules. The novel approach to assemble PAS is compared with traditional methods used for fabrication of porous alginate hydrogels. Finally, future perspectives and applications of PAS for advanced cell culture, tissue engineering, and drug testing are discussed. MDPI 2019-05-29 /pmc/articles/PMC6630714/ /pubmed/31146472 http://dx.doi.org/10.3390/mi10060357 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Sergeeva, Alena Vikulina, Anna S. Volodkin, Dmitry Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title | Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title_full | Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title_fullStr | Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title_full_unstemmed | Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title_short | Porous Alginate Scaffolds Assembled Using Vaterite CaCO(3) Crystals |
title_sort | porous alginate scaffolds assembled using vaterite caco(3) crystals |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630714/ https://www.ncbi.nlm.nih.gov/pubmed/31146472 http://dx.doi.org/10.3390/mi10060357 |
work_keys_str_mv | AT sergeevaalena porousalginatescaffoldsassembledusingvateritecaco3crystals AT vikulinaannas porousalginatescaffoldsassembledusingvateritecaco3crystals AT volodkindmitry porousalginatescaffoldsassembledusingvateritecaco3crystals |