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Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines

Background: Curcumin has numerous properties and is used in many preclinical conditions, including cancer. It has low bioavailability, while its derivative EF24 shows enhanced solubility. However, its effects have never been explored in adrenocortical tumor cell models. The efficacy of EF24 alone or...

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Autores principales: Bertazza, Loris, Barollo, Susi, Mari, Maria Elena, Faccio, Irene, Zorzan, Maira, Redaelli, Marco, Rubin, Beatrice, Armanini, Decio, Mian, Caterina, Pezzani, Raffaele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630722/
https://www.ncbi.nlm.nih.gov/pubmed/31212829
http://dx.doi.org/10.3390/molecules24122202
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author Bertazza, Loris
Barollo, Susi
Mari, Maria Elena
Faccio, Irene
Zorzan, Maira
Redaelli, Marco
Rubin, Beatrice
Armanini, Decio
Mian, Caterina
Pezzani, Raffaele
author_facet Bertazza, Loris
Barollo, Susi
Mari, Maria Elena
Faccio, Irene
Zorzan, Maira
Redaelli, Marco
Rubin, Beatrice
Armanini, Decio
Mian, Caterina
Pezzani, Raffaele
author_sort Bertazza, Loris
collection PubMed
description Background: Curcumin has numerous properties and is used in many preclinical conditions, including cancer. It has low bioavailability, while its derivative EF24 shows enhanced solubility. However, its effects have never been explored in adrenocortical tumor cell models. The efficacy of EF24 alone or combined with mitotane (reference drug for adrenocortical cancer) was evaluated in two adrenocortical tumor cell lines, SW13 and H295R. Method and Results: EF24 reduced cell viability with an IC50 (half maximal inhibitory concentration) of 6.5 ± 2.4 μM and 4.9 ± 2.8 μM for SW13 and H295R cells, respectively. Combination index (EF24 associated with mitotane) suggested an additivity effect in both cell lines. Cell cycle analysis revealed an increase in subG0/G1 phase, while motility assay showed a decrease in migratory cell capacity, and similarly, clonogenic assay indicated that EF24 could reduce colony numbers. Furthermore, Wnt/β-catenin, NF-κB, MAPK, and PI3k/Akt pathways were modulated by Western blot analysis when treating cells with EF24 alone or combined with mitotane. In addition, intracellular reactive oxygen species levels increased in both cell lines. Conclusion: This work analyzed EF24 in adrenocortical tumor cell lines for the first time. These results suggest that EF24 could potentially impact on adrenocortical tumors, laying the foundation for further research in animal models.
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spelling pubmed-66307222019-08-19 Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines Bertazza, Loris Barollo, Susi Mari, Maria Elena Faccio, Irene Zorzan, Maira Redaelli, Marco Rubin, Beatrice Armanini, Decio Mian, Caterina Pezzani, Raffaele Molecules Article Background: Curcumin has numerous properties and is used in many preclinical conditions, including cancer. It has low bioavailability, while its derivative EF24 shows enhanced solubility. However, its effects have never been explored in adrenocortical tumor cell models. The efficacy of EF24 alone or combined with mitotane (reference drug for adrenocortical cancer) was evaluated in two adrenocortical tumor cell lines, SW13 and H295R. Method and Results: EF24 reduced cell viability with an IC50 (half maximal inhibitory concentration) of 6.5 ± 2.4 μM and 4.9 ± 2.8 μM for SW13 and H295R cells, respectively. Combination index (EF24 associated with mitotane) suggested an additivity effect in both cell lines. Cell cycle analysis revealed an increase in subG0/G1 phase, while motility assay showed a decrease in migratory cell capacity, and similarly, clonogenic assay indicated that EF24 could reduce colony numbers. Furthermore, Wnt/β-catenin, NF-κB, MAPK, and PI3k/Akt pathways were modulated by Western blot analysis when treating cells with EF24 alone or combined with mitotane. In addition, intracellular reactive oxygen species levels increased in both cell lines. Conclusion: This work analyzed EF24 in adrenocortical tumor cell lines for the first time. These results suggest that EF24 could potentially impact on adrenocortical tumors, laying the foundation for further research in animal models. MDPI 2019-06-12 /pmc/articles/PMC6630722/ /pubmed/31212829 http://dx.doi.org/10.3390/molecules24122202 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bertazza, Loris
Barollo, Susi
Mari, Maria Elena
Faccio, Irene
Zorzan, Maira
Redaelli, Marco
Rubin, Beatrice
Armanini, Decio
Mian, Caterina
Pezzani, Raffaele
Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title_full Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title_fullStr Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title_full_unstemmed Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title_short Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines
title_sort biological effects of ef24, a curcumin derivative, alone or combined with mitotane in adrenocortical tumor cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630722/
https://www.ncbi.nlm.nih.gov/pubmed/31212829
http://dx.doi.org/10.3390/molecules24122202
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