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Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues

Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remai...

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Autores principales: Iimaa, Tuyajargal, Hirayama, Takaaki, Shirakigawa, Nana, Imai, Daisuke, Yamao, Takanobu, Yamashita, Yo-ichi, Baba, Hideo, Ijima, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630779/
https://www.ncbi.nlm.nih.gov/pubmed/31212711
http://dx.doi.org/10.3390/gels5020032
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author Iimaa, Tuyajargal
Hirayama, Takaaki
Shirakigawa, Nana
Imai, Daisuke
Yamao, Takanobu
Yamashita, Yo-ichi
Baba, Hideo
Ijima, Hiroyuki
author_facet Iimaa, Tuyajargal
Hirayama, Takaaki
Shirakigawa, Nana
Imai, Daisuke
Yamao, Takanobu
Yamashita, Yo-ichi
Baba, Hideo
Ijima, Hiroyuki
author_sort Iimaa, Tuyajargal
collection PubMed
description Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.
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spelling pubmed-66307792019-08-19 Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues Iimaa, Tuyajargal Hirayama, Takaaki Shirakigawa, Nana Imai, Daisuke Yamao, Takanobu Yamashita, Yo-ichi Baba, Hideo Ijima, Hiroyuki Gels Article Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells. MDPI 2019-06-11 /pmc/articles/PMC6630779/ /pubmed/31212711 http://dx.doi.org/10.3390/gels5020032 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iimaa, Tuyajargal
Hirayama, Takaaki
Shirakigawa, Nana
Imai, Daisuke
Yamao, Takanobu
Yamashita, Yo-ichi
Baba, Hideo
Ijima, Hiroyuki
Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title_full Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title_fullStr Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title_full_unstemmed Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title_short Antibacterial-Agent-Immobilized Gelatin Hydrogel as a 3D Scaffold for Natural and Bioengineered Tissues
title_sort antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630779/
https://www.ncbi.nlm.nih.gov/pubmed/31212711
http://dx.doi.org/10.3390/gels5020032
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