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Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties
3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630791/ https://www.ncbi.nlm.nih.gov/pubmed/31200501 http://dx.doi.org/10.3390/pharmaceutics11060277 |
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author | Arany, Petra Róka, Eszter Mollet, Laurent Coleman, Anthony W. Perret, Florent Kim, Beomjoon Kovács, Renátó Kazsoki, Adrienn Zelkó, Romána Gesztelyi, Rudolf Ujhelyi, Zoltán Fehér, Pálma Váradi, Judit Fenyvesi, Ferenc Vecsernyés, Miklós Bácskay, Ildikó |
author_facet | Arany, Petra Róka, Eszter Mollet, Laurent Coleman, Anthony W. Perret, Florent Kim, Beomjoon Kovács, Renátó Kazsoki, Adrienn Zelkó, Romána Gesztelyi, Rudolf Ujhelyi, Zoltán Fehér, Pálma Váradi, Judit Fenyvesi, Ferenc Vecsernyés, Miklós Bácskay, Ildikó |
author_sort | Arany, Petra |
collection | PubMed |
description | 3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The polyvalent test plates (PVTPs) are poly-lactic acid cylinders, 14 mm in diameter and 3 mm in height. The polymer ester backbone was surface modified by a series of ramified and linear oligoamines to increase its hydrophilicity and introduce a positive charge. The chemical modification was verified by FT-IR spectroscopy, showing the introduction of amide and amine functions, and contact angle measurements confirmed increased hydrophilicity. Morphology studies (SEM, optical microscopy) indicated that the modification of PVTP possessed a planar morphology with small pits. Positron annihilation lifetime spectroscopy demonstrated that the polymeric free volume decreased on modification. An MTT-based prolonged cytotoxicity test using Caco-2 cells showed that the PVTPs are non-toxic at the cellular level. The presence of surface oligoamines on the PVTPs reduced biofilm formation by Candida albicans SC5314 significantly. The results demonstrate that 3D printed objects may be modified at their surface by a simple amidation reaction, resulting in a reduced propensity for biofilm colonization and cellular toxicity. |
format | Online Article Text |
id | pubmed-6630791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66307912019-08-19 Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties Arany, Petra Róka, Eszter Mollet, Laurent Coleman, Anthony W. Perret, Florent Kim, Beomjoon Kovács, Renátó Kazsoki, Adrienn Zelkó, Romána Gesztelyi, Rudolf Ujhelyi, Zoltán Fehér, Pálma Váradi, Judit Fenyvesi, Ferenc Vecsernyés, Miklós Bácskay, Ildikó Pharmaceutics Article 3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The polyvalent test plates (PVTPs) are poly-lactic acid cylinders, 14 mm in diameter and 3 mm in height. The polymer ester backbone was surface modified by a series of ramified and linear oligoamines to increase its hydrophilicity and introduce a positive charge. The chemical modification was verified by FT-IR spectroscopy, showing the introduction of amide and amine functions, and contact angle measurements confirmed increased hydrophilicity. Morphology studies (SEM, optical microscopy) indicated that the modification of PVTP possessed a planar morphology with small pits. Positron annihilation lifetime spectroscopy demonstrated that the polymeric free volume decreased on modification. An MTT-based prolonged cytotoxicity test using Caco-2 cells showed that the PVTPs are non-toxic at the cellular level. The presence of surface oligoamines on the PVTPs reduced biofilm formation by Candida albicans SC5314 significantly. The results demonstrate that 3D printed objects may be modified at their surface by a simple amidation reaction, resulting in a reduced propensity for biofilm colonization and cellular toxicity. MDPI 2019-06-13 /pmc/articles/PMC6630791/ /pubmed/31200501 http://dx.doi.org/10.3390/pharmaceutics11060277 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arany, Petra Róka, Eszter Mollet, Laurent Coleman, Anthony W. Perret, Florent Kim, Beomjoon Kovács, Renátó Kazsoki, Adrienn Zelkó, Romána Gesztelyi, Rudolf Ujhelyi, Zoltán Fehér, Pálma Váradi, Judit Fenyvesi, Ferenc Vecsernyés, Miklós Bácskay, Ildikó Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title | Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title_full | Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title_fullStr | Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title_full_unstemmed | Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title_short | Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties |
title_sort | fused deposition modeling 3d printing: test platforms for evaluating post-fabrication chemical modifications and in-vitro biological properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630791/ https://www.ncbi.nlm.nih.gov/pubmed/31200501 http://dx.doi.org/10.3390/pharmaceutics11060277 |
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