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Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes

Maternal metabolic disorders in ewes induced by energy deficiency have a detrimental effect on the maternal health and lambs. However, the dynamic processes of metabolic disorders are unknown. Therefore, this study attempted to explore the dynamic changes of maternal metabolism based on metabolomics...

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Autores principales: Guo, Changzheng, Xue, Yanfeng, Seddik, Hossam-eldin, Yin, Yuyang, Hu, Fan, Mao, Shengyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630903/
https://www.ncbi.nlm.nih.gov/pubmed/31185597
http://dx.doi.org/10.3390/metabo9060112
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author Guo, Changzheng
Xue, Yanfeng
Seddik, Hossam-eldin
Yin, Yuyang
Hu, Fan
Mao, Shengyong
author_facet Guo, Changzheng
Xue, Yanfeng
Seddik, Hossam-eldin
Yin, Yuyang
Hu, Fan
Mao, Shengyong
author_sort Guo, Changzheng
collection PubMed
description Maternal metabolic disorders in ewes induced by energy deficiency have a detrimental effect on the maternal health and lambs. However, the dynamic processes of metabolic disorders are unknown. Therefore, this study attempted to explore the dynamic changes of maternal metabolism based on metabolomics approach during energy deficiency in pregnant ewes. Twenty pregnant Hu sheep were fed a basic diet or a 70% restricted basic diet. The HPLC-MS platform was applied to identify blood metabolites. Principal component analysis of blood samples based on their metabolic profile showed that blood samples of feed restriction group differed after the treatment. In particular, when comparing both groups, there were 120, 129, and 114 differential metabolites at day 5, day 10, and day 114 between the two groups, respectively. Enrichment analysis results showed that four metabolic pathways (glycerophospholipid metabolism, linoleic acid metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis) at day 5, four metabolic pathways (aminoacyl-tRNA biosynthesis, aminoacyl-tRNA biosynthesis, glycerophospholipid metabolism, and citrate cycle) at day 10, and nine metabolic pathways (aminoacyl-tRNA biosynthesis, synthesis and degradation of ketone bodies, glycerophospholipid metabolism, butanoate metabolism, linoleic acid metabolism, citrate cycle, alanine, aspartate and glutamate metabolism, valine, leucine and isoleucine biosynthesis, and arginine and proline metabolism) at day 15 were significantly enriched between the two groups. These findings revealed temporal changes of metabolic disorders in pregnant ewes caused by severe feed restriction, which may provide insights into mitigation measures.
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spelling pubmed-66309032019-08-19 Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes Guo, Changzheng Xue, Yanfeng Seddik, Hossam-eldin Yin, Yuyang Hu, Fan Mao, Shengyong Metabolites Article Maternal metabolic disorders in ewes induced by energy deficiency have a detrimental effect on the maternal health and lambs. However, the dynamic processes of metabolic disorders are unknown. Therefore, this study attempted to explore the dynamic changes of maternal metabolism based on metabolomics approach during energy deficiency in pregnant ewes. Twenty pregnant Hu sheep were fed a basic diet or a 70% restricted basic diet. The HPLC-MS platform was applied to identify blood metabolites. Principal component analysis of blood samples based on their metabolic profile showed that blood samples of feed restriction group differed after the treatment. In particular, when comparing both groups, there were 120, 129, and 114 differential metabolites at day 5, day 10, and day 114 between the two groups, respectively. Enrichment analysis results showed that four metabolic pathways (glycerophospholipid metabolism, linoleic acid metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis) at day 5, four metabolic pathways (aminoacyl-tRNA biosynthesis, aminoacyl-tRNA biosynthesis, glycerophospholipid metabolism, and citrate cycle) at day 10, and nine metabolic pathways (aminoacyl-tRNA biosynthesis, synthesis and degradation of ketone bodies, glycerophospholipid metabolism, butanoate metabolism, linoleic acid metabolism, citrate cycle, alanine, aspartate and glutamate metabolism, valine, leucine and isoleucine biosynthesis, and arginine and proline metabolism) at day 15 were significantly enriched between the two groups. These findings revealed temporal changes of metabolic disorders in pregnant ewes caused by severe feed restriction, which may provide insights into mitigation measures. MDPI 2019-06-10 /pmc/articles/PMC6630903/ /pubmed/31185597 http://dx.doi.org/10.3390/metabo9060112 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Changzheng
Xue, Yanfeng
Seddik, Hossam-eldin
Yin, Yuyang
Hu, Fan
Mao, Shengyong
Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title_full Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title_fullStr Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title_full_unstemmed Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title_short Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes
title_sort dynamic changes of plasma metabolome in response to severe feed restriction in pregnant ewes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630903/
https://www.ncbi.nlm.nih.gov/pubmed/31185597
http://dx.doi.org/10.3390/metabo9060112
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