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Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey

High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overla...

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Autores principales: Çağlayan, Kadriye, Roumi, Vahid, Gazel, Mona, Elçi, Eminur, Acioğlu, Mehtap, Mavric Plesko, Irena, Reynard, Jean-Sebastien, Maclot, Francois, Massart, Sebastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631170/
https://www.ncbi.nlm.nih.gov/pubmed/31035571
http://dx.doi.org/10.3390/pathogens8020057
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author Çağlayan, Kadriye
Roumi, Vahid
Gazel, Mona
Elçi, Eminur
Acioğlu, Mehtap
Mavric Plesko, Irena
Reynard, Jean-Sebastien
Maclot, Francois
Massart, Sebastien
author_facet Çağlayan, Kadriye
Roumi, Vahid
Gazel, Mona
Elçi, Eminur
Acioğlu, Mehtap
Mavric Plesko, Irena
Reynard, Jean-Sebastien
Maclot, Francois
Massart, Sebastien
author_sort Çağlayan, Kadriye
collection PubMed
description High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5′ UTR and 3′ UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.
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spelling pubmed-66311702019-08-19 Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey Çağlayan, Kadriye Roumi, Vahid Gazel, Mona Elçi, Eminur Acioğlu, Mehtap Mavric Plesko, Irena Reynard, Jean-Sebastien Maclot, Francois Massart, Sebastien Pathogens Article High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5′ UTR and 3′ UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies. MDPI 2019-04-27 /pmc/articles/PMC6631170/ /pubmed/31035571 http://dx.doi.org/10.3390/pathogens8020057 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Çağlayan, Kadriye
Roumi, Vahid
Gazel, Mona
Elçi, Eminur
Acioğlu, Mehtap
Mavric Plesko, Irena
Reynard, Jean-Sebastien
Maclot, Francois
Massart, Sebastien
Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title_full Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title_fullStr Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title_full_unstemmed Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title_short Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey
title_sort identification and characterization of a novel robigovirus species from sweet cherry in turkey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631170/
https://www.ncbi.nlm.nih.gov/pubmed/31035571
http://dx.doi.org/10.3390/pathogens8020057
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