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Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration

During wound healing, bacterial infections may prolong skin regeneration and tissue repair, causing delayed or incomplete healing. The therapeutic strategies currently used include general therapeutic modes, growth factors, skin substitutes, matrices and/or cell therapy. Among recent technologies, w...

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Autores principales: Ceresa, Chiara, Fracchia, Letizia, Marchetti, Alice, Rinaldi, Maurizio, Bosetti, Michela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631215/
https://www.ncbi.nlm.nih.gov/pubmed/31208032
http://dx.doi.org/10.3390/ma12121931
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author Ceresa, Chiara
Fracchia, Letizia
Marchetti, Alice
Rinaldi, Maurizio
Bosetti, Michela
author_facet Ceresa, Chiara
Fracchia, Letizia
Marchetti, Alice
Rinaldi, Maurizio
Bosetti, Michela
author_sort Ceresa, Chiara
collection PubMed
description During wound healing, bacterial infections may prolong skin regeneration and tissue repair, causing delayed or incomplete healing. The therapeutic strategies currently used include general therapeutic modes, growth factors, skin substitutes, matrices and/or cell therapy. Among recent technologies, wound dressing materials comprising silver nitrate or silver sulfadiazine as the antimicrobial agent are widespread, despite their known cytotoxicity. The aim of this work was to develop and evaluate the efficacy of gelatinous injectable biomaterials composed of collagen and alginates, enriched with silver against bacterial pathogens commonly involved in wound infections. To reduce cytotoxicity, silver was used as lactate and saccharinated salts. Results show that silver-enriched beads were effective against both Gram-positive and Gram-negative strains in a concentration-dependent manner. Silver addition was more active against Staphylococcus epidermidis than against Pseudomonas aeruginosa. The antibacterial activity was localized only in the area of contact with the beads at concentrations lower than 0.3 mM, whereas at higher concentrations a larger inhibition halo was observed. No cytotoxic effect on eukaryotic cells was seen both testing the materials’ extracts or the Ag-doped beads in contact tests. These results, although preliminary, suggest that these scaffolds are a promising approach for realizing injectable or spreadable functional biomaterials with antibacterial activity for applications in wound management.
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spelling pubmed-66312152019-08-19 Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration Ceresa, Chiara Fracchia, Letizia Marchetti, Alice Rinaldi, Maurizio Bosetti, Michela Materials (Basel) Article During wound healing, bacterial infections may prolong skin regeneration and tissue repair, causing delayed or incomplete healing. The therapeutic strategies currently used include general therapeutic modes, growth factors, skin substitutes, matrices and/or cell therapy. Among recent technologies, wound dressing materials comprising silver nitrate or silver sulfadiazine as the antimicrobial agent are widespread, despite their known cytotoxicity. The aim of this work was to develop and evaluate the efficacy of gelatinous injectable biomaterials composed of collagen and alginates, enriched with silver against bacterial pathogens commonly involved in wound infections. To reduce cytotoxicity, silver was used as lactate and saccharinated salts. Results show that silver-enriched beads were effective against both Gram-positive and Gram-negative strains in a concentration-dependent manner. Silver addition was more active against Staphylococcus epidermidis than against Pseudomonas aeruginosa. The antibacterial activity was localized only in the area of contact with the beads at concentrations lower than 0.3 mM, whereas at higher concentrations a larger inhibition halo was observed. No cytotoxic effect on eukaryotic cells was seen both testing the materials’ extracts or the Ag-doped beads in contact tests. These results, although preliminary, suggest that these scaffolds are a promising approach for realizing injectable or spreadable functional biomaterials with antibacterial activity for applications in wound management. MDPI 2019-06-15 /pmc/articles/PMC6631215/ /pubmed/31208032 http://dx.doi.org/10.3390/ma12121931 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ceresa, Chiara
Fracchia, Letizia
Marchetti, Alice
Rinaldi, Maurizio
Bosetti, Michela
Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title_full Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title_fullStr Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title_full_unstemmed Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title_short Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration
title_sort injectable scaffolds enriched with silver to inhibit bacterial invasion in tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631215/
https://www.ncbi.nlm.nih.gov/pubmed/31208032
http://dx.doi.org/10.3390/ma12121931
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