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Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals

Quantifying HIV Envelope (Env)-specific antibodies in HIV(+) plasma is useful for interpreting antibody dependent cellular cytotoxicity assay results. HIV Env, the only viral protein expressed on the surface of infected cells, has a native trimeric closed conformation on cells infected with wild-typ...

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Autores principales: Kant, Sanket, Zhang, Ningyu, Routy, Jean-Pierre, Tremblay, Cécile, Thomas, Réjean, Szabo, Jason, Côté, Pierre, Trottier, Benoit, LeBlanc, Roger, Rouleau, Danielle, Harris, Marianne, Dupuy, Franck P., Bernard, Nicole F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631318/
https://www.ncbi.nlm.nih.gov/pubmed/31141927
http://dx.doi.org/10.3390/v11060487
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author Kant, Sanket
Zhang, Ningyu
Routy, Jean-Pierre
Tremblay, Cécile
Thomas, Réjean
Szabo, Jason
Côté, Pierre
Trottier, Benoit
LeBlanc, Roger
Rouleau, Danielle
Harris, Marianne
Dupuy, Franck P.
Bernard, Nicole F.
author_facet Kant, Sanket
Zhang, Ningyu
Routy, Jean-Pierre
Tremblay, Cécile
Thomas, Réjean
Szabo, Jason
Côté, Pierre
Trottier, Benoit
LeBlanc, Roger
Rouleau, Danielle
Harris, Marianne
Dupuy, Franck P.
Bernard, Nicole F.
author_sort Kant, Sanket
collection PubMed
description Quantifying HIV Envelope (Env)-specific antibodies in HIV(+) plasma is useful for interpreting antibody dependent cellular cytotoxicity assay results. HIV Env, the only viral protein expressed on the surface of infected cells, has a native trimeric closed conformation on cells infected with wild-type HIV. However, CD4(+) uninfected bystander cells in HIV(+) cell cultures bind gp120 shed from HIV(+) cells exposing CD4-induced epitopes normally hidden in native Env. We used flow-cytometry based assays to quantify antibodies in HIV(+) plasma specific for native trimeric Env or gp120/CD4 conjugates using CEM.NKr.CCR5 (CEM) cells infected with HIV (iCEM) or coated with recombinant gp120 (cCEM), as a surrogate for gp120(+) HIV(-) bystander cells. Results from both assays were compared to those of a plate-based ELISA to monomeric gp120. The levels of Env-specific antibodies to cCEM and iCEM, measured by flow cytometry, and to gp120 by ELISA were positively correlated. More antibodies in HIV(+) plasma recognized the gp120 conformation exposed on cCEM than on iCEM. Comparisons of plasma from untreated progressors, treated progressors, and elite controllers revealed that antibodies to Env epitopes were the lowest in treated progressors. Plasma from elite controllers and untreated progressors had similarly high levels of Env-specific antibodies, despite elite controllers having undetectable HIV viral loads, while untreated progressors maintained high viral loads.
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spelling pubmed-66313182019-08-19 Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals Kant, Sanket Zhang, Ningyu Routy, Jean-Pierre Tremblay, Cécile Thomas, Réjean Szabo, Jason Côté, Pierre Trottier, Benoit LeBlanc, Roger Rouleau, Danielle Harris, Marianne Dupuy, Franck P. Bernard, Nicole F. Viruses Article Quantifying HIV Envelope (Env)-specific antibodies in HIV(+) plasma is useful for interpreting antibody dependent cellular cytotoxicity assay results. HIV Env, the only viral protein expressed on the surface of infected cells, has a native trimeric closed conformation on cells infected with wild-type HIV. However, CD4(+) uninfected bystander cells in HIV(+) cell cultures bind gp120 shed from HIV(+) cells exposing CD4-induced epitopes normally hidden in native Env. We used flow-cytometry based assays to quantify antibodies in HIV(+) plasma specific for native trimeric Env or gp120/CD4 conjugates using CEM.NKr.CCR5 (CEM) cells infected with HIV (iCEM) or coated with recombinant gp120 (cCEM), as a surrogate for gp120(+) HIV(-) bystander cells. Results from both assays were compared to those of a plate-based ELISA to monomeric gp120. The levels of Env-specific antibodies to cCEM and iCEM, measured by flow cytometry, and to gp120 by ELISA were positively correlated. More antibodies in HIV(+) plasma recognized the gp120 conformation exposed on cCEM than on iCEM. Comparisons of plasma from untreated progressors, treated progressors, and elite controllers revealed that antibodies to Env epitopes were the lowest in treated progressors. Plasma from elite controllers and untreated progressors had similarly high levels of Env-specific antibodies, despite elite controllers having undetectable HIV viral loads, while untreated progressors maintained high viral loads. MDPI 2019-05-28 /pmc/articles/PMC6631318/ /pubmed/31141927 http://dx.doi.org/10.3390/v11060487 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kant, Sanket
Zhang, Ningyu
Routy, Jean-Pierre
Tremblay, Cécile
Thomas, Réjean
Szabo, Jason
Côté, Pierre
Trottier, Benoit
LeBlanc, Roger
Rouleau, Danielle
Harris, Marianne
Dupuy, Franck P.
Bernard, Nicole F.
Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title_full Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title_fullStr Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title_full_unstemmed Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title_short Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
title_sort quantifying anti-hiv envelope-specific antibodies in plasma from hiv infected individuals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631318/
https://www.ncbi.nlm.nih.gov/pubmed/31141927
http://dx.doi.org/10.3390/v11060487
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