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Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus

Newcastle disease virus (NDV), an avian paramyxovirus, was shown to prefer to replicate in tumor cells instead of normal cells; however, this mechanism has not been fully elucidated. Exosomes play a crucial role in intercellular communication due to the bioactive substances they carry. Several studi...

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Autores principales: Zhou, Changluan, Tan, Lei, Sun, Yingjie, Qiu, Xusheng, Meng, Chunchun, Liao, Ying, Song, Cuiping, Liu, Weiwei, Nair, Venugopal, Ding, Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631457/
https://www.ncbi.nlm.nih.gov/pubmed/31174402
http://dx.doi.org/10.3390/v11060527
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author Zhou, Changluan
Tan, Lei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Liao, Ying
Song, Cuiping
Liu, Weiwei
Nair, Venugopal
Ding, Chan
author_facet Zhou, Changluan
Tan, Lei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Liao, Ying
Song, Cuiping
Liu, Weiwei
Nair, Venugopal
Ding, Chan
author_sort Zhou, Changluan
collection PubMed
description Newcastle disease virus (NDV), an avian paramyxovirus, was shown to prefer to replicate in tumor cells instead of normal cells; however, this mechanism has not been fully elucidated. Exosomes play a crucial role in intercellular communication due to the bioactive substances they carry. Several studies have shown that exosomes are involved in virus infections. However, the effect that exosomes have on NDV-infected tumor cells is not known. In this study, we focus on the role of exosomes secreted by NDV-infected HeLa cells in promoting NDV replication. Three miRNA candidates (miR-1273f, miR-1184, and miR-198) embraced by exosomes were associated with enhancing NDV-induced cytopathic effects on HeLa cells. Furthermore, luciferase assays, RT-qPCR, and enzyme-linked immunosorbent assay (ELISA) all demonstrated that these miRNAs could suppress interferon (IFN)-β gene expression. Enhanced NDV replication in HeLa cells was identified by Western blot and plaque assays. Based on these results, we speculate that NDV employed exosomes entry into neighboring cells, which carry miRNAs, resulting in inhibition of the IFN pathway and promotion of viral infection. To our knowledge, this is the first report on the involvement of NDV-employed exosomes in tumor cells, and as such, it provides new insights into the development of anti-tumor therapies.
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spelling pubmed-66314572019-08-19 Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus Zhou, Changluan Tan, Lei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Liao, Ying Song, Cuiping Liu, Weiwei Nair, Venugopal Ding, Chan Viruses Article Newcastle disease virus (NDV), an avian paramyxovirus, was shown to prefer to replicate in tumor cells instead of normal cells; however, this mechanism has not been fully elucidated. Exosomes play a crucial role in intercellular communication due to the bioactive substances they carry. Several studies have shown that exosomes are involved in virus infections. However, the effect that exosomes have on NDV-infected tumor cells is not known. In this study, we focus on the role of exosomes secreted by NDV-infected HeLa cells in promoting NDV replication. Three miRNA candidates (miR-1273f, miR-1184, and miR-198) embraced by exosomes were associated with enhancing NDV-induced cytopathic effects on HeLa cells. Furthermore, luciferase assays, RT-qPCR, and enzyme-linked immunosorbent assay (ELISA) all demonstrated that these miRNAs could suppress interferon (IFN)-β gene expression. Enhanced NDV replication in HeLa cells was identified by Western blot and plaque assays. Based on these results, we speculate that NDV employed exosomes entry into neighboring cells, which carry miRNAs, resulting in inhibition of the IFN pathway and promotion of viral infection. To our knowledge, this is the first report on the involvement of NDV-employed exosomes in tumor cells, and as such, it provides new insights into the development of anti-tumor therapies. MDPI 2019-06-06 /pmc/articles/PMC6631457/ /pubmed/31174402 http://dx.doi.org/10.3390/v11060527 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Changluan
Tan, Lei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Liao, Ying
Song, Cuiping
Liu, Weiwei
Nair, Venugopal
Ding, Chan
Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title_full Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title_fullStr Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title_full_unstemmed Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title_short Exosomes Carry microRNAs into Neighboring Cells to Promote Diffusive Infection of Newcastle Disease Virus
title_sort exosomes carry micrornas into neighboring cells to promote diffusive infection of newcastle disease virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631457/
https://www.ncbi.nlm.nih.gov/pubmed/31174402
http://dx.doi.org/10.3390/v11060527
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