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Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics
Kit-based assays, such as AbsoluteIDQ(TM) p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hamper...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631474/ https://www.ncbi.nlm.nih.gov/pubmed/31181753 http://dx.doi.org/10.3390/metabo9060109 |
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author | Quell, Jan D. Römisch-Margl, Werner Haid, Mark Krumsiek, Jan Skurk, Thomas Halama, Anna Stephan, Nisha Adamski, Jerzy Hauner, Hans Mook-Kanamori, Dennis Mohney, Robert P. Daniel, Hannelore Suhre, Karsten Kastenmüller, Gabi |
author_facet | Quell, Jan D. Römisch-Margl, Werner Haid, Mark Krumsiek, Jan Skurk, Thomas Halama, Anna Stephan, Nisha Adamski, Jerzy Hauner, Hans Mook-Kanamori, Dennis Mohney, Robert P. Daniel, Hannelore Suhre, Karsten Kastenmüller, Gabi |
author_sort | Quell, Jan D. |
collection | PubMed |
description | Kit-based assays, such as AbsoluteIDQ(TM) p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hampered by lack of functionally-relevant information on the detailed fatty acid side-chain compositions as only the total number of carbon atoms and double bonds is identified by the kit. To enable more substantiated interpretations, we characterized these PC sums using the side-chain resolving Lipidyzer(TM) platform, analyzing 223 samples in parallel to the AbsoluteIDQ(TM). Combining these datasets, we estimated the quantitative composition of PC sums and subsequently tested their replication in an independent cohort. We identified major constituents of 28 PC sums, revealing also various unexpected compositions. As an example, PC 16:0_22:5 accounted for more than 50% of the PC sum with in total 38 carbon atoms and 5 double bonds (PC aa 38:5). For 13 PC sums, we found relatively high abundances of odd-chain fatty acids. In conclusion, our study provides insights in PC compositions in human plasma, facilitating interpretation of existing epidemiological data sets and potentially enabling imputation of PC compositions for future meta-analyses of lipidomics data. |
format | Online Article Text |
id | pubmed-6631474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66314742019-08-19 Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics Quell, Jan D. Römisch-Margl, Werner Haid, Mark Krumsiek, Jan Skurk, Thomas Halama, Anna Stephan, Nisha Adamski, Jerzy Hauner, Hans Mook-Kanamori, Dennis Mohney, Robert P. Daniel, Hannelore Suhre, Karsten Kastenmüller, Gabi Metabolites Article Kit-based assays, such as AbsoluteIDQ(TM) p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hampered by lack of functionally-relevant information on the detailed fatty acid side-chain compositions as only the total number of carbon atoms and double bonds is identified by the kit. To enable more substantiated interpretations, we characterized these PC sums using the side-chain resolving Lipidyzer(TM) platform, analyzing 223 samples in parallel to the AbsoluteIDQ(TM). Combining these datasets, we estimated the quantitative composition of PC sums and subsequently tested their replication in an independent cohort. We identified major constituents of 28 PC sums, revealing also various unexpected compositions. As an example, PC 16:0_22:5 accounted for more than 50% of the PC sum with in total 38 carbon atoms and 5 double bonds (PC aa 38:5). For 13 PC sums, we found relatively high abundances of odd-chain fatty acids. In conclusion, our study provides insights in PC compositions in human plasma, facilitating interpretation of existing epidemiological data sets and potentially enabling imputation of PC compositions for future meta-analyses of lipidomics data. MDPI 2019-06-08 /pmc/articles/PMC6631474/ /pubmed/31181753 http://dx.doi.org/10.3390/metabo9060109 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Quell, Jan D. Römisch-Margl, Werner Haid, Mark Krumsiek, Jan Skurk, Thomas Halama, Anna Stephan, Nisha Adamski, Jerzy Hauner, Hans Mook-Kanamori, Dennis Mohney, Robert P. Daniel, Hannelore Suhre, Karsten Kastenmüller, Gabi Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title_full | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title_fullStr | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title_full_unstemmed | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title_short | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
title_sort | characterization of bulk phosphatidylcholine compositions in human plasma using side-chain resolving lipidomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631474/ https://www.ncbi.nlm.nih.gov/pubmed/31181753 http://dx.doi.org/10.3390/metabo9060109 |
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